Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 117921154

GI number: 117921154

Start: 3242653

End: 3244479

Strand: Reverse

Name: lcfB [H]

Synonym: Shewana3_2713

Alternate gene names: 117921154

Gene position: 3244479-3242653 (Counterclockwise)

Preceding gene: 117921155

Following gene: 117921153

Centisome position: 65.25

GC content: 52.55

Gene sequence:

>1827_bases
ATGGCTAGGGTGGAGGATGCGCAGCTCGGCCATGGGACATCTAGCGTCGAGCAAGCAAGCGATGCCAATATTTGTCGGCA
TTTAAAGCAGGCCGCCCATCACATTCCCCATCATCTTGCGGTTGCGGTGCAACAGGGCAAGGGTAAATCCCTTGCCAATC
TGACTTACCAAGAACTCGACTTTATTAGCCTCGATAAACAAAGCGATGCCATCGCCTTCGCGCTCAATGCCCATGGGCTG
ACGCGGGGGATGAAGGCGGTGCTGATGGTCACCCCGAGTCTGGATTTTTTTGCGTTAACTTTCGCCTTGTTCAAGGCGGG
AATTATTCCCGTACTGGTTGACCCGGGCATGGGGATTAAAAACCTTAAGCAATGTTTTATTGAAGCTGCGCCCGATGCCT
TTATCGGGATCCCTAAAGCCCATATCGCCAGACGCTTATTAGGCTGGGGCAAGGGCAGCGTGAAGCACCTTATCAATGTG
GATGGCAATCCAAGCGGACTGGCAGGCACTTTATCGCGCGTACTCACGGGCGCGCCAAGTTTAGCGACGATACTCTTATC
AACTGCTAAGTCGTTTACAACGAAGGCATCTGCGGCAAACTTGCCGGAACAGGGCGAGTACCCAATGGCACTGCTTAGGC
ATGACGAGATGGCGGCGATTCTGTTTACCAGCGGCAGCACGGGGACGCCGAAAGGCGTTGTCTATAGTCACGGCATGTTT
GAGGCGCAAATTCAGGCGCTAAAGCAAGATTATGGCATTGCACATGGTGAGCGTGATTTGGCGACATTCCCGCTATTCTC
CCTCTTTGGTCCTGCGCTCGGTATGGCATCGATTGTACCTGAGATGGATGCCAGCAAACCTATTACGGCCAATCCTGAAT
TTTTATTTGCCGCCATTGAGAAGTACCAATGCAGCAATATCTTTGTTAATCCAGCCTTGCTTGAACGTTTAGGTCGCGCC
GGTGAGCAAACCGAATCGAAAAAGCAGCACAAACTCAGCAGTGTGAAACGCGTGATTTCCGCAGGGGCGCCCGCGACCAT
TGCCTCGATTGCCCGTTTTAGCAAAATGCTCGGTGTCGGCGTGCCTGTACTCAACTCCTACGGCGCCACTGAATCCTTAC
CCATCAGCATGATCGCCAGCGACGAACTATTGACCACGACTCAAACCACGGACAATGGCGGTGGAATTTGTGTCGGCCGC
GCCATCGATGGTGTCAGTATCGAGATTATCGGCATCACTGAAGCTGATATCCCTGAGTGGGATAATGGCTTGCGTCTTAA
CGCGGGGGAAATTGGCGAGATAGTGGTAACAGGCCCTATGGTGAGCCAGTCCTATTATCGCCGCGAAAAGGCAACGACGG
CGGCCAAAATTTGGGACCGCGAGCGGCAAACCTTTAGGCATAGAATGGGTGACCTTGGCTACCTGGACGACAGCGGCCGA
TTGTGGATGTGTGGCCGCAAAGCCCATAGAGTGGATGCCACCCAAGGCGGGCAGTGTATTAAACGTTACTATTCCATTCC
GAGTGAGCGTATTTTTAATACCCATCCGAATGTGAAGCGCTCGGCGTTAGTGGGGATCAAAGTGAAGGGGCAAGGTGGCG
TGTGTGAGATTGAACCACTCATCTGCATCGAGCTTGACCAATCCTTGGTGTGCAATAAGAGCGCGCAGCTCTATCAAGAG
TTGATGGCGATTGCCGAGCAGTATAGCCAAACTCAGGGGATTCGGCGTTTTCTCATTCATCCCGATTTCCCCGTGGATGT
GCGCCACAATGCCAAAATATTTAGAGAAAAATTAGCCGTTTGGGCGCAATCGCAAACCAAAGGCTGA

Upstream 100 bases:

>100_bases
TGGCTGAGGCGCAAACGCCTGAGCCAGAAGTACAGCCCGTTGAAACGGAACAGGTCGCAGAGCCTGAGGCAGAGACTCAA
GCGCCACAGGCCGCACATTA

Downstream 100 bases:

>100_bases
TTTCAATTTAGCTAATTTTTAACTTAAATAGGGTCGAACTTTCGACTGTTTGTTCGTGGTATGTTTTGCCGCGAGAGGTG
CCTTGTTGCACTTAATCACT

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MARVEDAQLGHGTSSVEQASDANICRHLKQAAHHIPHHLAVAVQQGKGKSLANLTYQELDFISLDKQSDAIAFALNAHGL
TRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIKNLKQCFIEAAPDAFIGIPKAHIARRLLGWGKGSVKHLINV
DGNPSGLAGTLSRVLTGAPSLATILLSTAKSFTTKASAANLPEQGEYPMALLRHDEMAAILFTSGSTGTPKGVVYSHGMF
EAQIQALKQDYGIAHGERDLATFPLFSLFGPALGMASIVPEMDASKPITANPEFLFAAIEKYQCSNIFVNPALLERLGRA
GEQTESKKQHKLSSVKRVISAGAPATIASIARFSKMLGVGVPVLNSYGATESLPISMIASDELLTTTQTTDNGGGICVGR
AIDGVSIEIIGITEADIPEWDNGLRLNAGEIGEIVVTGPMVSQSYYRREKATTAAKIWDRERQTFRHRMGDLGYLDDSGR
LWMCGRKAHRVDATQGGQCIKRYYSIPSERIFNTHPNVKRSALVGIKVKGQGGVCEIEPLICIELDQSLVCNKSAQLYQE
LMAIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAVWAQSQTKG

Sequences:

>Translated_608_residues
MARVEDAQLGHGTSSVEQASDANICRHLKQAAHHIPHHLAVAVQQGKGKSLANLTYQELDFISLDKQSDAIAFALNAHGL
TRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIKNLKQCFIEAAPDAFIGIPKAHIARRLLGWGKGSVKHLINV
DGNPSGLAGTLSRVLTGAPSLATILLSTAKSFTTKASAANLPEQGEYPMALLRHDEMAAILFTSGSTGTPKGVVYSHGMF
EAQIQALKQDYGIAHGERDLATFPLFSLFGPALGMASIVPEMDASKPITANPEFLFAAIEKYQCSNIFVNPALLERLGRA
GEQTESKKQHKLSSVKRVISAGAPATIASIARFSKMLGVGVPVLNSYGATESLPISMIASDELLTTTQTTDNGGGICVGR
AIDGVSIEIIGITEADIPEWDNGLRLNAGEIGEIVVTGPMVSQSYYRREKATTAAKIWDRERQTFRHRMGDLGYLDDSGR
LWMCGRKAHRVDATQGGQCIKRYYSIPSERIFNTHPNVKRSALVGIKVKGQGGVCEIEPLICIELDQSLVCNKSAQLYQE
LMAIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAVWAQSQTKG
>Mature_607_residues
ARVEDAQLGHGTSSVEQASDANICRHLKQAAHHIPHHLAVAVQQGKGKSLANLTYQELDFISLDKQSDAIAFALNAHGLT
RGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIKNLKQCFIEAAPDAFIGIPKAHIARRLLGWGKGSVKHLINVD
GNPSGLAGTLSRVLTGAPSLATILLSTAKSFTTKASAANLPEQGEYPMALLRHDEMAAILFTSGSTGTPKGVVYSHGMFE
AQIQALKQDYGIAHGERDLATFPLFSLFGPALGMASIVPEMDASKPITANPEFLFAAIEKYQCSNIFVNPALLERLGRAG
EQTESKKQHKLSSVKRVISAGAPATIASIARFSKMLGVGVPVLNSYGATESLPISMIASDELLTTTQTTDNGGGICVGRA
IDGVSIEIIGITEADIPEWDNGLRLNAGEIGEIVVTGPMVSQSYYRREKATTAAKIWDRERQTFRHRMGDLGYLDDSGRL
WMCGRKAHRVDATQGGQCIKRYYSIPSERIFNTHPNVKRSALVGIKVKGQGGVCEIEPLICIELDQSLVCNKSAQLYQEL
MAIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAVWAQSQTKG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI145693145, Length=517, Percent_Identity=23.21083172147, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=310, Percent_Identity=25.4838709677419, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 65802; Mature: 65670

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVEDAQLGHGTSSVEQASDANICRHLKQAAHHIPHHLAVAVQQGKGKSLANLTYQELD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCHHHCHHHHCC
FISLDKQSDAIAFALNAHGLTRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIK
EEEECCCCCEEEEEEECCHHHHCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHH
NLKQCFIEAAPDAFIGIPKAHIARRLLGWGKGSVKHLINVDGNPSGLAGTLSRVLTGAPS
HHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCH
LATILLSTAKSFTTKASAANLPEQGEYPMALLRHDEMAAILFTSGSTGTPKGVVYSHGMF
HHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCHH
EAQIQALKQDYGIAHGERDLATFPLFSLFGPALGMASIVPEMDASKPITANPEFLFAAIE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KYQCSNIFVNPALLERLGRAGEQTESKKQHKLSSVKRVISAGAPATIASIARFSKMLGVG
HHCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VPVLNSYGATESLPISMIASDELLTTTQTTDNGGGICVGRAIDGVSIEIIGITEADIPEW
CHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEEECCEEEEEEEECCCCCCCC
DNGLRLNAGEIGEIVVTGPMVSQSYYRREKATTAAKIWDRERQTFRHRMGDLGYLDDSGR
CCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
LWMCGRKAHRVDATQGGQCIKRYYSIPSERIFNTHPNVKRSALVGIKVKGQGGVCEIEPL
EEEECCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEECCE
ICIELDQSLVCNKSAQLYQELMAIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAV
EEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WAQSQTKG
HHHCCCCC
>Mature Secondary Structure 
ARVEDAQLGHGTSSVEQASDANICRHLKQAAHHIPHHLAVAVQQGKGKSLANLTYQELD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCHHHCHHHHCC
FISLDKQSDAIAFALNAHGLTRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIK
EEEECCCCCEEEEEEECCHHHHCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHH
NLKQCFIEAAPDAFIGIPKAHIARRLLGWGKGSVKHLINVDGNPSGLAGTLSRVLTGAPS
HHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCH
LATILLSTAKSFTTKASAANLPEQGEYPMALLRHDEMAAILFTSGSTGTPKGVVYSHGMF
HHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCHH
EAQIQALKQDYGIAHGERDLATFPLFSLFGPALGMASIVPEMDASKPITANPEFLFAAIE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
KYQCSNIFVNPALLERLGRAGEQTESKKQHKLSSVKRVISAGAPATIASIARFSKMLGVG
HHCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VPVLNSYGATESLPISMIASDELLTTTQTTDNGGGICVGRAIDGVSIEIIGITEADIPEW
CHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEEEEECCEEEEEEEECCCCCCCC
DNGLRLNAGEIGEIVVTGPMVSQSYYRREKATTAAKIWDRERQTFRHRMGDLGYLDDSGR
CCCEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
LWMCGRKAHRVDATQGGQCIKRYYSIPSERIFNTHPNVKRSALVGIKVKGQGGVCEIEPL
EEEECCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEECCE
ICIELDQSLVCNKSAQLYQELMAIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAV
EEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WAQSQTKG
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]