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The map label for this gene is vat [H]

Identifier: 117921133

GI number: 117921133

Start: 3218902

End: 3219576

Strand: Direct

Name: vat [H]

Synonym: Shewana3_2692

Alternate gene names: 117921133

Gene position: 3218902-3219576 (Clockwise)

Preceding gene: 117921132

Following gene: 117921134

Centisome position: 64.74

GC content: 50.96

Gene sequence:

>675_bases
ATGCCGACGCATTCCCCATCCCAACTGGGACCTAACCCGAATGACAAATCCCCCTTAAAAGGATTCCCTCAGGTGGGTTT
TCTGAAGAACTTTATTAGCCGCGACAACATTATCGTCGGCGATTACACCTATTACGACGACCCAGCAGGGCCAGAGCGTT
TCGAGTCAAACGTGCTTTATCACTTTGACTTTATTGGCGATAAGCTGATTATCGGTAAATTTTGTGCCATTGCACGGGAC
GTTAAATTCATTATGAATGGCGCCAATCACCAAGTATCGGGCTTTTCCACTTATCCCTTTTACATCTTCGGCAATGGCTG
GGAGAAGGCAACACCTAAGCCCGAGGATTTACCCTTTAAGGGCGACACCCGCATTGGCCATGATGTGTGGATAGGTTATA
ACGCCACCATTATGCCCGGCGTCAACGTCGGCCACGGCGCCATTATCGCCAGCCAAGCCGTAGTCACTAAGGATGTGCCA
CCCTATGCCATCGTCGGCGGTAACCCCGCGACTGTGATTAAATTGCGATTTGATCCCGAGGTAATAGACACATTACTCGC
CATTGCGTGGTGGGACTGGCCAATTGAGAAAATCAGCGAGCATTTACCTGCCATTGTGGGCGCCGATCTTAGCCAATTAG
CCCGCTTGGCCGATGGGATACACACGCGGCCCTAA

Upstream 100 bases:

>100_bases
CACCAGCAGGTGTAACTGGGCTTTCCTGTTATTAACTGTGCGCGACTCTTAACGGCGCTACAACCCAATTCTTAGCCATT
GAATAATAAGGGAGGCAAAC

Downstream 100 bases:

>100_bases
GCGTTGGCGGCAGAAAACAACGCACAGAACTTCCCCAAAAACACGGCAAAGCGCAATTTTGTACAAAAGTGTCGAGGCGT
TCTGGGTTACTATCTGGCAA

Product: streptogramin A acetyl transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPTHSPSQLGPNPNDKSPLKGFPQVGFLKNFISRDNIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIARD
VKFIMNGANHQVSGFSTYPFYIFGNGWEKATPKPEDLPFKGDTRIGHDVWIGYNATIMPGVNVGHGAIIASQAVVTKDVP
PYAIVGGNPATVIKLRFDPEVIDTLLAIAWWDWPIEKISEHLPAIVGADLSQLARLADGIHTRP

Sequences:

>Translated_224_residues
MPTHSPSQLGPNPNDKSPLKGFPQVGFLKNFISRDNIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIARD
VKFIMNGANHQVSGFSTYPFYIFGNGWEKATPKPEDLPFKGDTRIGHDVWIGYNATIMPGVNVGHGAIIASQAVVTKDVP
PYAIVGGNPATVIKLRFDPEVIDTLLAIAWWDWPIEKISEHLPAIVGADLSQLARLADGIHTRP
>Mature_223_residues
PTHSPSQLGPNPNDKSPLKGFPQVGFLKNFISRDNIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIARDV
KFIMNGANHQVSGFSTYPFYIFGNGWEKATPKPEDLPFKGDTRIGHDVWIGYNATIMPGVNVGHGAIIASQAVVTKDVPP
YAIVGGNPATVIKLRFDPEVIDTLLAIAWWDWPIEKISEHLPAIVGADLSQLARLADGIHTRP

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=50, Percent_Identity=60, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 24690; Mature: 24559

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTHSPSQLGPNPNDKSPLKGFPQVGFLKNFISRDNIIVGDYTYYDDPAGPERFESNVLY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCEEECCCCCCHHHHHCCEEE
HFDFIGDKLIIGKFCAIARDVKFIMNGANHQVSGFSTYPFYIFGNGWEKATPKPEDLPFK
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCC
GDTRIGHDVWIGYNATIMPGVNVGHGAIIASQAVVTKDVPPYAIVGGNPATVIKLRFDPE
CCCCCCCEEEECCCCEECCCCCCCCCEEEECCEEEECCCCCEEEECCCCEEEEEEEECHH
VIDTLLAIAWWDWPIEKISEHLPAIVGADLSQLARLADGIHTRP
HHHHHHHHHHCCCCHHHHHHHCCCHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PTHSPSQLGPNPNDKSPLKGFPQVGFLKNFISRDNIIVGDYTYYDDPAGPERFESNVLY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCEEECCCCCCHHHHHCCEEE
HFDFIGDKLIIGKFCAIARDVKFIMNGANHQVSGFSTYPFYIFGNGWEKATPKPEDLPFK
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCC
GDTRIGHDVWIGYNATIMPGVNVGHGAIIASQAVVTKDVPPYAIVGGNPATVIKLRFDPE
CCCCCCCEEEECCCCEECCCCCCCCCEEEECCEEEECCCCCEEEECCCCEEEEEEEECHH
VIDTLLAIAWWDWPIEKISEHLPAIVGADLSQLARLADGIHTRP
HHHHHHHHHHCCCCHHHHHHHCCCHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]