Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 117921132

GI number: 117921132

Start: 3217902

End: 3218795

Strand: Direct

Name: ada [H]

Synonym: Shewana3_2691

Alternate gene names: 117921132

Gene position: 3217902-3218795 (Clockwise)

Preceding gene: 117921131

Following gene: 117921133

Centisome position: 64.72

GC content: 51.79

Gene sequence:

>894_bases
ATGACCCAAGATTACGAGCGAATTGCGCGTGCCATTGACTTTATTCGTCAGCATGTGGGTCATCAACCCTCACTGGCCGA
GATTGCGGCGCACGTCCATTTAAGTGAATACCATTTTCAGCGGCTCTTTAGTCGCTGGGCGGGGATCACTCCCAAACGTT
ATTTGCAGGCCTTGACTCTTGAGCGGGCTAAACAGTTATTGCAGCAGCCGTCCCACTCGACCTTGAGTGCCAGTTATGAG
TTAGGCCTGAGCAGTGGCTCGCGCTTATACGATCATTTTGTACAACTCGAGGCGGTCACCCCCTTTGAATATCGCCAGCA
GGGTTTAGGGATCAGCATTGCCTATGGCTATCATTCCACGCCCTTCGGTTTGGCCTTTATCGCCCAAACGGCACGGGGCA
TTTGCCAGTTTGATTTTGTCGATATGCAGCAAATTCCATCGCCATTGGAACGTTTAAAGCAAAGCTGGAGTCAGGCCACC
ATTTATGAGGACACGGCGCAAACGGCAAGCTTAATTGATCACCTCTTTAGCCAGTACTTTGCGGGTTCGAGTGCCGCAGC
GCTGAATTTAACAGCACCTAATACTCAGGCAATTCAGCAAGGCGAGCCTGCCCCGTTATCACTCTGGGTAAAAGGCACTA
ACTTTCAACTTAATGTGTGGCGCGCTCTGCTGAATTTACCTAAGGGAGAGGTCGCCTCCTACGGTGATATTGCCAAGGCC
ATTGGTAAACCTAAGGCCTCACGCGCCGTCGGTACGGCGATTGGTGCCAATCCGGTGGCATTACTTATCCCCTGCCATAG
AGTGCTACGCCAAAGTGGTGAACTTGGCGGTTACCGCTGGGGTGAAACCCGTAAACATGCCATATTGGCAAGGGAAGCGG
CCTATTGTGAGTAA

Upstream 100 bases:

>100_bases
GCGCTATATCTTGCCTCCGATGCGGCCAGTTTTACCACAGGGATAGCCTTATTGGTCGATGGTGGTGTGTCCATCTGTAA
AACCTAGGGAATGGATAACG

Downstream 100 bases:

>100_bases
AAGATCCACCAGCAGGTGTAACTGGGCTTTCCTGTTATTAACTGTGCGCGACTCTTAACGGCGCTACAACCCAATTCTTA
GCCATTGAATAATAAGGGAG

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase / transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTQDYERIARAIDFIRQHVGHQPSLAEIAAHVHLSEYHFQRLFSRWAGITPKRYLQALTLERAKQLLQQPSHSTLSASYE
LGLSSGSRLYDHFVQLEAVTPFEYRQQGLGISIAYGYHSTPFGLAFIAQTARGICQFDFVDMQQIPSPLERLKQSWSQAT
IYEDTAQTASLIDHLFSQYFAGSSAAALNLTAPNTQAIQQGEPAPLSLWVKGTNFQLNVWRALLNLPKGEVASYGDIAKA
IGKPKASRAVGTAIGANPVALLIPCHRVLRQSGELGGYRWGETRKHAILAREAAYCE

Sequences:

>Translated_297_residues
MTQDYERIARAIDFIRQHVGHQPSLAEIAAHVHLSEYHFQRLFSRWAGITPKRYLQALTLERAKQLLQQPSHSTLSASYE
LGLSSGSRLYDHFVQLEAVTPFEYRQQGLGISIAYGYHSTPFGLAFIAQTARGICQFDFVDMQQIPSPLERLKQSWSQAT
IYEDTAQTASLIDHLFSQYFAGSSAAALNLTAPNTQAIQQGEPAPLSLWVKGTNFQLNVWRALLNLPKGEVASYGDIAKA
IGKPKASRAVGTAIGANPVALLIPCHRVLRQSGELGGYRWGETRKHAILAREAAYCE
>Mature_296_residues
TQDYERIARAIDFIRQHVGHQPSLAEIAAHVHLSEYHFQRLFSRWAGITPKRYLQALTLERAKQLLQQPSHSTLSASYEL
GLSSGSRLYDHFVQLEAVTPFEYRQQGLGISIAYGYHSTPFGLAFIAQTARGICQFDFVDMQQIPSPLERLKQSWSQATI
YEDTAQTASLIDHLFSQYFAGSSAAALNLTAPNTQAIQQGEPAPLSLWVKGTNFQLNVWRALLNLPKGEVASYGDIAKAI
GKPKASRAVGTAIGANPVALLIPCHRVLRQSGELGGYRWGETRKHAILAREAAYCE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=75, Percent_Identity=48, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788542, Length=270, Percent_Identity=34.8148148148148, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787596, Length=181, Percent_Identity=32.5966850828729, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=47.5609756097561, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=47.5609756097561, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137554, Length=194, Percent_Identity=30.9278350515464, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32955; Mature: 32824

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDYERIARAIDFIRQHVGHQPSLAEIAAHVHLSEYHFQRLFSRWAGITPKRYLQALTL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERAKQLLQQPSHSTLSASYELGLSSGSRLYDHFVQLEAVTPFEYRQQGLGISIAYGYHST
HHHHHHHHCCCCCCCHHHHEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCC
PFGLAFIAQTARGICQFDFVDMQQIPSPLERLKQSWSQATIYEDTAQTASLIDHLFSQYF
CHHHHHHHHHHCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGSSAAALNLTAPNTQAIQQGEPAPLSLWVKGTNFQLNVWRALLNLPKGEVASYGDIAKA
CCCCCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHH
IGKPKASRAVGTAIGANPVALLIPCHRVLRQSGELGGYRWGETRKHAILAREAAYCE
HCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQDYERIARAIDFIRQHVGHQPSLAEIAAHVHLSEYHFQRLFSRWAGITPKRYLQALTL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERAKQLLQQPSHSTLSASYELGLSSGSRLYDHFVQLEAVTPFEYRQQGLGISIAYGYHST
HHHHHHHHCCCCCCCHHHHEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCC
PFGLAFIAQTARGICQFDFVDMQQIPSPLERLKQSWSQATIYEDTAQTASLIDHLFSQYF
CHHHHHHHHHHCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGSSAAALNLTAPNTQAIQQGEPAPLSLWVKGTNFQLNVWRALLNLPKGEVASYGDIAKA
CCCCCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHH
IGKPKASRAVGTAIGANPVALLIPCHRVLRQSGELGGYRWGETRKHAILAREAAYCE
HCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]