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The map label for this gene is folD [H]

Identifier: 117921104

GI number: 117921104

Start: 3179719

End: 3180573

Strand: Direct

Name: folD [H]

Synonym: Shewana3_2663

Alternate gene names: 117921104

Gene position: 3179719-3180573 (Clockwise)

Preceding gene: 117921097

Following gene: 117921106

Centisome position: 63.95

GC content: 48.89

Gene sequence:

>855_bases
ATGACAGCCCAAATAATCGACGGCAAGGCGATTGCTCAATCCATCCGCACACAACTTCGCGAAAAAGTAACCGCCCGCAA
AGAGGCTGGACAACGCGTACCAGGTTTAGCCGTCATTCTCGTGGGAGCCGATCCCGCTTCTCAGGTTTATGTTGGTAGTA
AACGTAAAGCCTGTGAAGAAGTGGGATTTATTTCACGCTCTTACGATCTCGATACCAGTTATACCGAAGAAGCATTATTA
GCCCTTATCGACGAACTCAATGATGATCCGACGATCGACGGTATCTTAGTACAGCTTCCTCTGCCTGCGCATATCGAAGA
CTCTAAAGTGATCGAGCGTATTCGCCCCGATAAAGATGTCGACGGTTTCCACCCCTATAACGTTGGCCGTTTAGCCCAGC
GCATTCCCGTATTACGTTCATGCACCCCAATGGGGATCATGACCTTAATCAAATCAACGGGTGTAGATACCTATGGATTA
GATGCCGTTGTAGTAGGCGCCTCAAACATTGTCGGTCGACCAATGACACTGGAGCTATTGCTTGCGGGCTGCACCACAAC
CACATGCCATCGTTTTACAAAAAATCTCGAACAAAAAATCCGTCAGGCAGATTTAGTGGTCGTTGCCGTGGGTAAACCCG
GCTTTATCCCTGGTGAATGGATTAAACCCGGCGCTATTGTTATCGATGTGGGCATCAACCGCTTAGAAAACGGCACCTTA
GTGGGCGATGTGCAATACGATGTTGCTGCCCAAAACGCCAGCTTTATCACCCCAGTACCCGGTGGTGTCGGTCCTATGAC
GATTGCCAGCTTACTTGAAAATACCCTGTATGCTGCAGAGCAATATCACGACTAA

Upstream 100 bases:

>100_bases
CATGACACACCCGCAACGCCCTGTTAAAATAGCCTCGATTGCCACTCTCTGTGTTAACGACAGTTTTCACCATCGCCCAT
ATAAAGGATATCCTACACCC

Downstream 100 bases:

>100_bases
TCAGCTTCGCCATCGCGTAGATAATGACTTAAATGATGGCGTAAGTTATGGCGTAAATCGCAGCATTTAAACCTGCTATT
TAGCCATCTAAGCTCCAGTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQIIDGKAIAQSIRTQLREKVTARKEAGQRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDTSYTEEALL
ALIDELNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRQADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLENGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Sequences:

>Translated_284_residues
MTAQIIDGKAIAQSIRTQLREKVTARKEAGQRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDTSYTEEALL
ALIDELNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRQADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLENGTL
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
>Mature_283_residues
TAQIIDGKAIAQSIRTQLREKVTARKEAGQRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDTSYTEEALLA
LIDELNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLD
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEQKIRQADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLENGTLV
GDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=46.3087248322148, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.5374149659864, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.1768707482993, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI36796743, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=70.7746478873239, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=39.1156462585034, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.6107382550336, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.5087108013937, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=315, Percent_Identity=26.3492063492064, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=48.4848484848485, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=48.4848484848485, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=45.1724137931035, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=45.1724137931035, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=45.1724137931035, Blast_Score=230, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=45.1724137931035, Blast_Score=230, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30576; Mature: 30444

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIIDGKAIAQSIRTQLREKVTARKEAGQRVPGLAVILVGADPASQVYVGSKRKACEE
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLDTSYTEEALLALIDELNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRQADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLENGTL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQIIDGKAIAQSIRTQLREKVTARKEAGQRVPGLAVILVGADPASQVYVGSKRKACEE
CCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLDTSYTEEALLALIDELNDDPTIDGILVQLPLPAHIEDSKVIERIRPDKDV
HCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIKSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEQKIRQADLVVVAVGKPGFIPGEWIKPGAIVIDVGINRLENGTL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVQYDVAAQNASFITPVPGGVGPMTIASLLENTLYAAEQYHD
EEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA