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The map label for this gene is pspF [H]

Identifier: 117921090

GI number: 117921090

Start: 3162093

End: 3163181

Strand: Direct

Name: pspF [H]

Synonym: Shewana3_2649

Alternate gene names: 117921090

Gene position: 3162093-3163181 (Clockwise)

Preceding gene: 117921081

Following gene: 117921091

Centisome position: 63.6

GC content: 51.06

Gene sequence:

>1089_bases
GTGGATAATAAATTTCAGCAAGACAACCTCATCGGCCAATCGAACGCCCTACTCGAAGTGTTAGAGCATGTGTCACAGAT
AGCGCCGCTTTCTAAACCTGTTCTCATTATTGGTGAACGGGGTACGGGTAAGGAGCTGATTGCTGAACGCTTACATTATC
TGTCTAAACGCTGGGATCAAAGTTTTATTAAGCTAAATTGTTCATCTTTGAGTGAAAATTTGCTAGAAAGTGAATTATTC
GGCCATGAGTCAGGTGCCTTTACTGGCGCCAAAGGCAAACACGAAGGCCGTTTTGAGCGCGCCGACGGTGGCACTCTATT
CCTCGATGAGTTAGCCAACACCTCGGGATTAATCCAAGAAAAACTGCTACGGGTCATCGAATACGGTGAATTTGAACGGG
TCGGCGGCAGTAAAACCATTCAAGCGGATGTGCGCCTTATCTGCGCGGCTAACGAGGATTTACCCTCACTGGCCGACGCC
GGCGAATTTAGGGCCGACTTGCTCGACCGCTTAGCGTTCGATGTGATCACCTTGCCGCCACTGCGTTGCCGCCCCGAAGA
TATCATGACCTTAGCCGAATACTTTGCCGTGGGCATGGCGCGGCAACTTAAGCTGGAACTCTTCAGTGGCTTCAGTGCCA
GCGCAGTCGATCAGCTGATGAGCCATGACTGGCCGGGGAATATCCGTGAACTTAAGAACGTGGTCGAGCGCAGTGTGTAT
CGCAATAGTGGTGAGAACCACCCTATTGAGCAGATTATTCTCGACCCCTTTGCCTCTCCCTATCGCCCCACAACGCGAGT
GCGTACCCGTGAGCGCCAAATCTCCGCACCTGAAGCGCCGCTGAGCGCGCCCGTGATCACGTCGGCCAAAACCGATACCG
ACACGGCCGCCATTGCAGAGTCCATGACCAATCCGTTTAGTTTTCCTTTGGACTTTAAAGAGCAAACCGAACGTTATGAG
ATGGAGCTTATCCAGCAAGCCTTGTCGGCCAGTCAGTACAATCAAAAGAAGACGGCTGAAATCCTTGGCTTAAGCTATCA
CCAGCTGCGCGGCATTCTGAAAAAGTACAATTTGCTCGATAAAGCATAA

Upstream 100 bases:

>100_bases
TCATAAAGATAAACATATTCTTAACTTTTTCTTTATTTTATGGCTCAAGCCACTTATTATCATTTTCACCAAAATTTAGC
GCGAAAGACTAAAAAATATC

Downstream 100 bases:

>100_bases
GCAGATTGCCCACGGGCGAGATGCACTGCTAAAATAGCCGTTCATAGTCGATAAAATAATGATTTATAAATGAGTGTGCT
AATAAGACGCCTGTGCCTAT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTIQADVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLELFSGFSASAVDQLMSHDWPGNIRELKNVVERSVY
RNSGENHPIEQIILDPFASPYRPTTRVRTRERQISAPEAPLSAPVITSAKTDTDTAAIAESMTNPFSFPLDFKEQTERYE
MELIQQALSASQYNQKKTAEILGLSYHQLRGILKKYNLLDKA

Sequences:

>Translated_362_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTIQADVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLELFSGFSASAVDQLMSHDWPGNIRELKNVVERSVY
RNSGENHPIEQIILDPFASPYRPTTRVRTRERQISAPEAPLSAPVITSAKTDTDTAAIAESMTNPFSFPLDFKEQTERYE
MELIQQALSASQYNQKKTAEILGLSYHQLRGILKKYNLLDKA
>Mature_362_residues
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTIQADVRLICAANEDLPSLADA
GEFRADLLDRLAFDVITLPPLRCRPEDIMTLAEYFAVGMARQLKLELFSGFSASAVDQLMSHDWPGNIRELKNVVERSVY
RNSGENHPIEQIILDPFASPYRPTTRVRTRERQISAPEAPLSAPVITSAKTDTDTAAIAESMTNPFSFPLDFKEQTERYE
MELIQQALSASQYNQKKTAEILGLSYHQLRGILKKYNLLDKA

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=352, Percent_Identity=57.9545454545455, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=50, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788550, Length=358, Percent_Identity=38.5474860335196, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1790437, Length=239, Percent_Identity=48.5355648535565, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=48.7704918032787, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=361, Percent_Identity=35.1800554016621, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789233, Length=359, Percent_Identity=37.0473537604457, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790299, Length=352, Percent_Identity=36.6477272727273, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788905, Length=237, Percent_Identity=42.1940928270042, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=41.2, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787583, Length=352, Percent_Identity=32.3863636363636, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=34.3220338983051, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081858, Length=211, Percent_Identity=33.175355450237, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40532; Mature: 40532

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SFIKLNCSSLSENLLESELFGHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTIQADVRLICAANEDLPSLADAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LRCRPEDIMTLAEYFAVGMARQLKLELFSGFSASAVDQLMSHDWPGNIRELKNVVERSVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNSGENHPIEQIILDPFASPYRPTTRVRTRERQISAPEAPLSAPVITSAKTDTDTAAIAE
HCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHH
SMTNPFSFPLDFKEQTERYEMELIQQALSASQYNQKKTAEILGLSYHQLRGILKKYNLLD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHC
KA
CC
>Mature Secondary Structure
MDNKFQQDNLIGQSNALLEVLEHVSQIAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SFIKLNCSSLSENLLESELFGHESGAFTGAKGKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTIQADVRLICAANEDLPSLADAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
LRCRPEDIMTLAEYFAVGMARQLKLELFSGFSASAVDQLMSHDWPGNIRELKNVVERSVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNSGENHPIEQIILDPFASPYRPTTRVRTRERQISAPEAPLSAPVITSAKTDTDTAAIAE
HCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHH
SMTNPFSFPLDFKEQTERYEMELIQQALSASQYNQKKTAEILGLSYHQLRGILKKYNLLD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]