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The map label for this gene is ahpF [H]

Identifier: 117920978

GI number: 117920978

Start: 3034462

End: 3035361

Strand: Reverse

Name: ahpF [H]

Synonym: Shewana3_2537

Alternate gene names: 117920978

Gene position: 3035361-3034462 (Counterclockwise)

Preceding gene: 117920980

Following gene: 117920977

Centisome position: 61.05

GC content: 48.0

Gene sequence:

>900_bases
ATGCAAGTCGAAATTGTCATTATTGGTGGTAGTTTTGCTGGGTTAGCAGCCGCTTTGCAATTAGCAAGGGCGCAGCGAAA
GGTTGTGGTCATTGATGCTAATCAACCACGGAATAGGTTTGCCCATGCCTCACATGGGGTGTTTGGCCTAGATGGTAAAA
CGCCAGCGGAAATAAGAACGACTGTCCTTGCACAGTTGGAGCAGTATCCAAGTTTTTCGTTGCAGGAAACACAAGCCATT
GGTGTCGAACAAACACAAACGGGTTATGCGGTCTTAACGGCTGACGGTATGAATATTCGCTGTCAGAGGTTGATTTTAGC
TATGGGTATCCAAGATACATTGCCAAATATTGAGGGAGTAGCGACATATTGGGGGAAAGCCGTTGCTCACTGTCCTTATT
GCCATGGTTATGAATTGCGGGGTAAATCCTTGGGGGTTCTGGCAAGTGGTGAATTAGCCATGCATCAAGCCCTTATGCTG
CCTGATTGGGGGCCGACGACATTATTTACGCAACAAGCCTTTGTTCCCAACGATGGCGAACGACATGCCCTTGCCAAACG
GCAAGTACAAATTGAAACCACCCCAATAGTTTCAGTGCATGGTGATGGTGAATTGCTCACCCACGTGGTACTCGCAGATG
GGCGCACGGTTGCATTACAAGGTTTATTTGTTGCGCCGCAAATCCAGCTGCCAACGCCTCTGATTGAGATGTTAGGTTTA
GCGCTTGAAGCGGGCCCACTTGGTAATATTGTTAAAGTGGATGCGCAAAAGGAAAGTTCTTTGTTGGGGGTGTTTGTTGC
AGGTGATCTATCCAACCAGATGCAAAGTGCAACACTTGCCATCGCCTCGGGAACTATGGCGGGAATAGCGGCTCACCGTA
GCCTTATTTTTACTTCCTAA

Upstream 100 bases:

>100_bases
TTTCGTCGAAGCATATCATGAAACTTTTGAAGTTACATGTGTTGATTCTCACAATATTATCATGTAACAATAAAAGTTAC
ACAAATAGAAGGGGTGATTT

Downstream 100 bases:

>100_bases
CTGACAAGTGCTCATTTTGAACAAGTGCTATAGACGCACGTTAATCCCGATTGTTTATAACGTCTCACAGTGGATTACTA
TTTGTCATGGTTTGCTGTAG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQVEIVIIGGSFAGLAAALQLARAQRKVVVIDANQPRNRFAHASHGVFGLDGKTPAEIRTTVLAQLEQYPSFSLQETQAI
GVEQTQTGYAVLTADGMNIRCQRLILAMGIQDTLPNIEGVATYWGKAVAHCPYCHGYELRGKSLGVLASGELAMHQALML
PDWGPTTLFTQQAFVPNDGERHALAKRQVQIETTPIVSVHGDGELLTHVVLADGRTVALQGLFVAPQIQLPTPLIEMLGL
ALEAGPLGNIVKVDAQKESSLLGVFVAGDLSNQMQSATLAIASGTMAGIAAHRSLIFTS

Sequences:

>Translated_299_residues
MQVEIVIIGGSFAGLAAALQLARAQRKVVVIDANQPRNRFAHASHGVFGLDGKTPAEIRTTVLAQLEQYPSFSLQETQAI
GVEQTQTGYAVLTADGMNIRCQRLILAMGIQDTLPNIEGVATYWGKAVAHCPYCHGYELRGKSLGVLASGELAMHQALML
PDWGPTTLFTQQAFVPNDGERHALAKRQVQIETTPIVSVHGDGELLTHVVLADGRTVALQGLFVAPQIQLPTPLIEMLGL
ALEAGPLGNIVKVDAQKESSLLGVFVAGDLSNQMQSATLAIASGTMAGIAAHRSLIFTS
>Mature_299_residues
MQVEIVIIGGSFAGLAAALQLARAQRKVVVIDANQPRNRFAHASHGVFGLDGKTPAEIRTTVLAQLEQYPSFSLQETQAI
GVEQTQTGYAVLTADGMNIRCQRLILAMGIQDTLPNIEGVATYWGKAVAHCPYCHGYELRGKSLGVLASGELAMHQALML
PDWGPTTLFTQQAFVPNDGERHALAKRQVQIETTPIVSVHGDGELLTHVVLADGRTVALQGLFVAPQIQLPTPLIEMLGL
ALEAGPLGNIVKVDAQKESSLLGVFVAGDLSNQMQSATLAIASGTMAGIAAHRSLIFTS

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 31765; Mature: 31765

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVEIVIIGGSFAGLAAALQLARAQRKVVVIDANQPRNRFAHASHGVFGLDGKTPAEIRT
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEECCCCCHHHHHH
TVLAQLEQYPSFSLQETQAIGVEQTQTGYAVLTADGMNIRCQRLILAMGIQDTLPNIEGV
HHHHHHHHCCCCCCCHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHCCHHHCCCCCHH
ATYWGKAVAHCPYCHGYELRGKSLGVLASGELAMHQALMLPDWGPTTLFTQQAFVPNDGE
HHHHHHHHHCCCCCCCEEECCCEEEEEECCCHHHHHEEECCCCCCCCEEEEECCCCCCCC
RHALAKRQVQIETTPIVSVHGDGELLTHVVLADGRTVALQGLFVAPQIQLPTPLIEMLGL
HHHHHHEEEEEEECEEEEEECCCCEEEEEEEECCCEEEEEEEEECCCEECCCHHHHHHHH
ALEAGPLGNIVKVDAQKESSLLGVFVAGDLSNQMQSATLAIASGTMAGIAAHRSLIFTS
EECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHEEEEEECCCHHHHHHHCEEEECC
>Mature Secondary Structure
MQVEIVIIGGSFAGLAAALQLARAQRKVVVIDANQPRNRFAHASHGVFGLDGKTPAEIRT
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEECCCCCHHHHHH
TVLAQLEQYPSFSLQETQAIGVEQTQTGYAVLTADGMNIRCQRLILAMGIQDTLPNIEGV
HHHHHHHHCCCCCCCHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHCCHHHCCCCCHH
ATYWGKAVAHCPYCHGYELRGKSLGVLASGELAMHQALMLPDWGPTTLFTQQAFVPNDGE
HHHHHHHHHCCCCCCCEEECCCEEEEEECCCHHHHHEEECCCCCCCCEEEEECCCCCCCC
RHALAKRQVQIETTPIVSVHGDGELLTHVVLADGRTVALQGLFVAPQIQLPTPLIEMLGL
HHHHHHEEEEEEECEEEEEECCCCEEEEEEEECCCEEEEEEEEECCCEECCCHHHHHHHH
ALEAGPLGNIVKVDAQKESSLLGVFVAGDLSNQMQSATLAIASGTMAGIAAHRSLIFTS
EECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHEEEEEECCCHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]