Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 117920902

GI number: 117920902

Start: 2948078

End: 2949370

Strand: Reverse

Name: mltB [H]

Synonym: Shewana3_2459

Alternate gene names: 117920902

Gene position: 2949370-2948078 (Counterclockwise)

Preceding gene: 117920905

Following gene: 117920901

Centisome position: 59.32

GC content: 52.44

Gene sequence:

>1293_bases
TTGATCAAACTGAAAGGAATGTCCTATCTTGCCGCCAGCATCGCCGCGTGTGTGAGCTTAAGTGCGTGTTCGAGCGCGCC
AACATCGAGCGGTAATAGTCAAACCGCGATCGCCGAGACGCAGGCCGCCGTGCAAGAAATGCCGCTCAGTGACACCATCG
CCGCCCATGCTGCGGCGTTTCCGACCTGTGTGGCTAACCTACAGGAGCGCGCACGTACCGAAGGCTTATCCGAAGCCACT
ATCCAAGACACAGTCGCCAGTTTGCAGTTTGTGCCTAAAGTGATTGAGCTTGACCAAGCCCAGCCCGAGTTTAGCCAAAC
CTTCAACAACTATTTTACCAAGCGGGCGACCGATTGGCGGGTGAATGAAGGCCGCCGTTTACTGAAAAAACACCGCGCGC
TGCTCGATAAATTGGCGCAGGAATACGGTGTACCGCCACAATATATTCTGTCTTTCTGGGGGCTTGAGACTAACTACGGT
TCCTACAAAGGCAAAATGTCCGTGTTAGATTCCCTTGCTACCTTGGCCTGTGAGCCGCGTCGTAGTGGCTATTTTACGAC
TGAACTGATGCAAGCGCTTAAGTTAAAAGAAAAATACGGCTTTGATAAGAGCACTATGGTGGGCTCTTGGGCTGGCGCCA
TGGGGCACACCCAGTTTATGCCCTCGGCCTATGCTAAATATGCCATCGATGGTGATGGCGACGGTAAGGCCGATTTGTGG
AATAGCACCGAAGATGCACTCACCTCGGCGGCGAACTTTTTGCAACACTTAGGCTGGCAACGCAATGAGCGTTGGGGGCG
TGAAGTGATATTGCCGCGCAATTTCAACTATGAAAACCTTGGTGCCAAACAGGCGCAGCCGCTATCCCAATGGGCGGCGC
AATCCGTAGTGCAAAGTAATGGATTGCCTTTACCCGCTATCAATATGAAGGCGGCGCTGTATTTGCCCTCGGGTCATACT
GGCCCCGCGTTTTTAGGCTATGAGAACTTCAATGTGATCATGCGTTGGAACCGCTCTGAGTTTTACGCCATTACCGTCGG
TCATTTGGCCGATAGAATTAATGGCGGCGAACCACTAAAAGTGGCACCGCCCGAGCAGCCACGTTTGAGTCGTGAGCAAA
TCAAACAGTTACAAACCAAGCTGAATGAATCAGGCTTCGATGTCGGAAAACCAGACGGCGTACTTGGGCGTAACTCTTCG
GCGGGGATCCAAGCATTCCAGCGCAGTAAGAATATGATTGCCGACGGTTTCCCAAGCCCTGAAGTCTTTAGCGCTCTGGG
GATTTCACTCTAA

Upstream 100 bases:

>100_bases
TTTATGGCTAAATATCGTATCTCTCTTGGCTTGGGGGGATCCACCTCTTGCCTTGCTCAGCTAAGATACATTCTTAGCGC
TGACTGTAGGAGTAATTGAT

Downstream 100 bases:

>100_bases
AGTCTTTAGGTTGAGTCGTACTGCCTGCGAATATTGCCGCAAGTGGCCTATGGTTTACTCAACCCATGCCACTTCAGGCG
TGGGGCTTGCTAATCCGCAG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIKLKGMSYLAASIAACVSLSACSSAPTSSGNSQTAIAETQAAVQEMPLSDTIAAHAAAFPTCVANLQERARTEGLSEAT
IQDTVASLQFVPKVIELDQAQPEFSQTFNNYFTKRATDWRVNEGRRLLKKHRALLDKLAQEYGVPPQYILSFWGLETNYG
SYKGKMSVLDSLATLACEPRRSGYFTTELMQALKLKEKYGFDKSTMVGSWAGAMGHTQFMPSAYAKYAIDGDGDGKADLW
NSTEDALTSAANFLQHLGWQRNERWGREVILPRNFNYENLGAKQAQPLSQWAAQSVVQSNGLPLPAINMKAALYLPSGHT
GPAFLGYENFNVIMRWNRSEFYAITVGHLADRINGGEPLKVAPPEQPRLSREQIKQLQTKLNESGFDVGKPDGVLGRNSS
AGIQAFQRSKNMIADGFPSPEVFSALGISL

Sequences:

>Translated_430_residues
MIKLKGMSYLAASIAACVSLSACSSAPTSSGNSQTAIAETQAAVQEMPLSDTIAAHAAAFPTCVANLQERARTEGLSEAT
IQDTVASLQFVPKVIELDQAQPEFSQTFNNYFTKRATDWRVNEGRRLLKKHRALLDKLAQEYGVPPQYILSFWGLETNYG
SYKGKMSVLDSLATLACEPRRSGYFTTELMQALKLKEKYGFDKSTMVGSWAGAMGHTQFMPSAYAKYAIDGDGDGKADLW
NSTEDALTSAANFLQHLGWQRNERWGREVILPRNFNYENLGAKQAQPLSQWAAQSVVQSNGLPLPAINMKAALYLPSGHT
GPAFLGYENFNVIMRWNRSEFYAITVGHLADRINGGEPLKVAPPEQPRLSREQIKQLQTKLNESGFDVGKPDGVLGRNSS
AGIQAFQRSKNMIADGFPSPEVFSALGISL
>Mature_430_residues
MIKLKGMSYLAASIAACVSLSACSSAPTSSGNSQTAIAETQAAVQEMPLSDTIAAHAAAFPTCVANLQERARTEGLSEAT
IQDTVASLQFVPKVIELDQAQPEFSQTFNNYFTKRATDWRVNEGRRLLKKHRALLDKLAQEYGVPPQYILSFWGLETNYG
SYKGKMSVLDSLATLACEPRRSGYFTTELMQALKLKEKYGFDKSTMVGSWAGAMGHTQFMPSAYAKYAIDGDGDGKADLW
NSTEDALTSAANFLQHLGWQRNERWGREVILPRNFNYENLGAKQAQPLSQWAAQSVVQSNGLPLPAINMKAALYLPSGHT
GPAFLGYENFNVIMRWNRSEFYAITVGHLADRINGGEPLKVAPPEQPRLSREQIKQLQTKLNESGFDVGKPDGVLGRNSS
AGIQAFQRSKNMIADGFPSPEVFSALGISL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=355, Percent_Identity=30.7042253521127, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47111; Mature: 47111

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLKGMSYLAASIAACVSLSACSSAPTSSGNSQTAIAETQAAVQEMPLSDTIAAHAAAF
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
PTCVANLQERARTEGLSEATIQDTVASLQFVPKVIELDQAQPEFSQTFNNYFTKRATDWR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
VNEGRRLLKKHRALLDKLAQEYGVPPQYILSFWGLETNYGSYKGKMSVLDSLATLACEPR
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
RSGYFTTELMQALKLKEKYGFDKSTMVGSWAGAMGHTQFMPSAYAKYAIDGDGDGKADLW
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHEEEECCCCCCCCCCC
NSTEDALTSAANFLQHLGWQRNERWGREVILPRNFNYENLGAKQAQPLSQWAAQSVVQSN
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHCHHHHHHHHHHHHHHC
GLPLPAINMKAALYLPSGHTGPAFLGYENFNVIMRWNRSEFYAITVGHLADRINGGEPLK
CCCCCCCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCEEEEEHHHHHHHCCCCCCEE
VAPPEQPRLSREQIKQLQTKLNESGFDVGKPDGVLGRNSSAGIQAFQRSKNMIADGFPSP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCH
EVFSALGISL
HHHHHHCCCC
>Mature Secondary Structure
MIKLKGMSYLAASIAACVSLSACSSAPTSSGNSQTAIAETQAAVQEMPLSDTIAAHAAAF
CEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
PTCVANLQERARTEGLSEATIQDTVASLQFVPKVIELDQAQPEFSQTFNNYFTKRATDWR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
VNEGRRLLKKHRALLDKLAQEYGVPPQYILSFWGLETNYGSYKGKMSVLDSLATLACEPR
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
RSGYFTTELMQALKLKEKYGFDKSTMVGSWAGAMGHTQFMPSAYAKYAIDGDGDGKADLW
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHEEEECCCCCCCCCCC
NSTEDALTSAANFLQHLGWQRNERWGREVILPRNFNYENLGAKQAQPLSQWAAQSVVQSN
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHCHHHHHHHHHHHHHHC
GLPLPAINMKAALYLPSGHTGPAFLGYENFNVIMRWNRSEFYAITVGHLADRINGGEPLK
CCCCCCCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCEEEEEHHHHHHHCCCCCCEE
VAPPEQPRLSREQIKQLQTKLNESGFDVGKPDGVLGRNSSAGIQAFQRSKNMIADGFPSP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCH
EVFSALGISL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]