Click here to switch to the map view.

The map label for this gene is estA [H]

Identifier: 117920898

GI number: 117920898

Start: 2943859

End: 2944524

Strand: Reverse

Name: estA [H]

Synonym: Shewana3_2455

Alternate gene names: 117920898

Gene position: 2944524-2943859 (Counterclockwise)

Preceding gene: 117920899

Following gene: 117920897

Centisome position: 59.22

GC content: 49.85

Gene sequence:

>666_bases
ATGACACTTGAACGTATTGTGATTGAGCCTCAAGTTGAGGCGACGGCGGCGGTGATTTGGTTACACGGTTTAGGGGACTC
GGGCGCAGGGTTTGCACCTGTGGTTCCGGCGCTAGGATTGCCTGCGGGGCATAGCATTCGCTTTATCTTCCCCCATGCGC
CAGAACAAGCGGTGACCATCAACGGCGGCTACATTATGCGCGCTTGGTATGACATCAAAAGCATGGATTTGCACGATAGA
GCCGATATGCAGGGTGTGATGGCCTCGGAGCTGAGTGTGCAATCCTTGATTGATGAACAAATCGCGGCTGGTATTCCGAG
TGAACGCATCGTCTTAGCCGGATTTAGCCAAGGCGGGGTGATGAGCCTATTTACGGGATTACGTTATCCTCAAAAACTCG
CTGGCATTATGGCACTTTCCTGTTATTTACCGACGGGTGATGTATTACCCAGCCAATTAAGTGTCGCCAATGCGAAAACG
CCTATTTTACATCAGCACGGTGAACAGGATGATGTGGTGCCATTATCCGCAGGTTTATTAGCGAAGAATGCGCTAGAGGC
GGGTGGATATTCGGTGCAATGGCAAACCTATCCTATGCCACATTCGGTCATTCCGGTGCAATTAAAAGCAATAAGCACTT
GGTTACAAGAGTGTTTTGAAATGTAG

Upstream 100 bases:

>100_bases
AACCAGCTCGACGCTTTGCAAGCTCTCCTCGGCATTGATTATCTGTAAAGCGCTCGCGGGGCGAGAATAATAACTTTTGA
TTTATAAGGGATTTGTCAGT

Downstream 100 bases:

>100_bases
AAAAATCTAAATGCTGATAAAAACATCATGCGGTAAAGTGTGTTTTTACCGTTTGATGTTTTTTATTCAGAACAATAAGA
TGTTTTGTCATTAATTGCTA

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTLERIVIEPQVEATAAVIWLHGLGDSGAGFAPVVPALGLPAGHSIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDLHDR
ADMQGVMASELSVQSLIDEQIAAGIPSERIVLAGFSQGGVMSLFTGLRYPQKLAGIMALSCYLPTGDVLPSQLSVANAKT
PILHQHGEQDDVVPLSAGLLAKNALEAGGYSVQWQTYPMPHSVIPVQLKAISTWLQECFEM

Sequences:

>Translated_221_residues
MTLERIVIEPQVEATAAVIWLHGLGDSGAGFAPVVPALGLPAGHSIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDLHDR
ADMQGVMASELSVQSLIDEQIAAGIPSERIVLAGFSQGGVMSLFTGLRYPQKLAGIMALSCYLPTGDVLPSQLSVANAKT
PILHQHGEQDDVVPLSAGLLAKNALEAGGYSVQWQTYPMPHSVIPVQLKAISTWLQECFEM
>Mature_220_residues
TLERIVIEPQVEATAAVIWLHGLGDSGAGFAPVVPALGLPAGHSIRFIFPHAPEQAVTINGGYIMRAWYDIKSMDLHDRA
DMQGVMASELSVQSLIDEQIAAGIPSERIVLAGFSQGGVMSLFTGLRYPQKLAGIMALSCYLPTGDVLPSQLSVANAKTP
ILHQHGEQDDVVPLSAGLLAKNALEAGGYSVQWQTYPMPHSVIPVQLKAISTWLQECFEM

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=206, Percent_Identity=38.8349514563107, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI5453722, Length=209, Percent_Identity=39.7129186602871, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI20270341, Length=220, Percent_Identity=30.4545454545455, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17508057, Length=211, Percent_Identity=38.3886255924171, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323147, Length=213, Percent_Identity=36.150234741784, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45553055, Length=192, Percent_Identity=39.5833333333333, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI45553057, Length=192, Percent_Identity=39.5833333333333, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI21357257, Length=192, Percent_Identity=39.5833333333333, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI161078179, Length=213, Percent_Identity=33.8028169014084, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 23747; Mature: 23616

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLERIVIEPQVEATAAVIWLHGLGDSGAGFAPVVPALGLPAGHSIRFIFPHAPEQAVTI
CCCCEEEECCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEE
NGGYIMRAWYDIKSMDLHDRADMQGVMASELSVQSLIDEQIAAGIPSERIVLAGFSQGGV
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
MSLFTGLRYPQKLAGIMALSCYLPTGDVLPSQLSVANAKTPILHQHGEQDDVVPLSAGLL
HHHHHHCCCHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCHHHCCCCCCCEEEEHHHHH
AKNALEAGGYSVQWQTYPMPHSVIPVQLKAISTWLQECFEM
HHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLERIVIEPQVEATAAVIWLHGLGDSGAGFAPVVPALGLPAGHSIRFIFPHAPEQAVTI
CCCEEEECCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEE
NGGYIMRAWYDIKSMDLHDRADMQGVMASELSVQSLIDEQIAAGIPSERIVLAGFSQGGV
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
MSLFTGLRYPQKLAGIMALSCYLPTGDVLPSQLSVANAKTPILHQHGEQDDVVPLSAGLL
HHHHHHCCCHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCHHHCCCCCCCEEEEHHHHH
AKNALEAGGYSVQWQTYPMPHSVIPVQLKAISTWLQECFEM
HHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA