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The map label for this gene is gloB

Identifier: 117920812

GI number: 117920812

Start: 2847156

End: 2847959

Strand: Direct

Name: gloB

Synonym: Shewana3_2369

Alternate gene names: 117920812

Gene position: 2847156-2847959 (Clockwise)

Preceding gene: 117920809

Following gene: 117920813

Centisome position: 57.26

GC content: 49.5

Gene sequence:

>804_bases
ATGCTCACTATAACAGCGATTAATGCCTTTAATGACAATTATATCTGGGTGTTGCGACAAGACTCCCAGCAAGCCGTGTA
TGTGGTTGACCCAGGTGATGCCAATGTCGTGCTCGACTACTTGCACGCCCAGCGACTCTCGTTAGCGGGTATTTTGATCA
CCCATCACCATAGGGACCACACGGGTGGGATCGCCGCCCTAACGGACCACGTAAAACAGACCACAGGCCATGACTTAGCG
GTTTATGGTCCACAAAGTGAAGATATTCAAGGCATCAATCAGCCAATTGAGCCAACGCTGAGTGATTCACTCACTCTGCC
TTTTATTGACGCGCCAGTGCGTATCCTGTCCGTTCCTGGTCATACGGCTGGGCATATTGCCTATCTGGTTGATGACGCAT
TATTTTGCGGTGATACCCTCTTTAGCGCAGGTTGCGGCCGCTTATTTGAGGGCACACCAAGCCAAATGTGGCAATCCTTA
AGTCTGCTCGCGGCGCTGCCCGATGAAACCCGAGTCTACTGCGCCCATGAATACACTTTATCAAATCTGAAGTTTGCCCA
AACGGTCGATACTGATAATGAAGCGCTTAACGCCTATGTGGAGGAAGCAAGCACCCTTCGTGCCCAAGGCAAAGCCACCA
TTCCCTCCACGATCGGATTGGAGCGAGCCATTAATCCCTTTCTGCGCCCTTTGTCGCCCACCATAGTCAACAGCATAAAA
AATCAGTTTTGTGATCAAGATCTGACTAAAGCAGATGAACTGACCTGTTTTACCCTACTCAGGCAGTGGAAAGATATTTT
TTAG

Upstream 100 bases:

>100_bases
CATGAGCAATTGTCATGGGCAATCGCAACAGTCGCCAAGTGAGCCCCTCTTATGGATAATAACCTTATCTTCAAAACGAA
ATACAGCGATCGGCGGTGCT

Downstream 100 bases:

>100_bases
TAAAAAACGATCTATTTATGGCTGAAATTTATACAGTTTTTCCCACTTTTGATGATGTAAATCAAAAGTGACCCTACAAG
ATGTACGATAATTTAGGGAC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLTITAINAFNDNYIWVLRQDSQQAVYVVDPGDANVVLDYLHAQRLSLAGILITHHHRDHTGGIAALTDHVKQTTGHDLA
VYGPQSEDIQGINQPIEPTLSDSLTLPFIDAPVRILSVPGHTAGHIAYLVDDALFCGDTLFSAGCGRLFEGTPSQMWQSL
SLLAALPDETRVYCAHEYTLSNLKFAQTVDTDNEALNAYVEEASTLRAQGKATIPSTIGLERAINPFLRPLSPTIVNSIK
NQFCDQDLTKADELTCFTLLRQWKDIF

Sequences:

>Translated_267_residues
MLTITAINAFNDNYIWVLRQDSQQAVYVVDPGDANVVLDYLHAQRLSLAGILITHHHRDHTGGIAALTDHVKQTTGHDLA
VYGPQSEDIQGINQPIEPTLSDSLTLPFIDAPVRILSVPGHTAGHIAYLVDDALFCGDTLFSAGCGRLFEGTPSQMWQSL
SLLAALPDETRVYCAHEYTLSNLKFAQTVDTDNEALNAYVEEASTLRAQGKATIPSTIGLERAINPFLRPLSPTIVNSIK
NQFCDQDLTKADELTCFTLLRQWKDIF
>Mature_267_residues
MLTITAINAFNDNYIWVLRQDSQQAVYVVDPGDANVVLDYLHAQRLSLAGILITHHHRDHTGGIAALTDHVKQTTGHDLA
VYGPQSEDIQGINQPIEPTLSDSLTLPFIDAPVRILSVPGHTAGHIAYLVDDALFCGDTLFSAGCGRLFEGTPSQMWQSL
SLLAALPDETRVYCAHEYTLSNLKFAQTVDTDNEALNAYVEEASTLRAQGKATIPSTIGLERAINPFLRPLSPTIVNSIK
NQFCDQDLTKADELTCFTLLRQWKDIF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=274, Percent_Identity=35.036496350365, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI94538322, Length=274, Percent_Identity=35.036496350365, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI14150041, Length=235, Percent_Identity=38.7234042553192, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI116642887, Length=235, Percent_Identity=34.8936170212766, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI21703352, Length=235, Percent_Identity=34.8936170212766, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI46361987, Length=219, Percent_Identity=37.4429223744292, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1786406, Length=266, Percent_Identity=42.8571428571429, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787158, Length=162, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=279, Percent_Identity=31.8996415770609, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320478, Length=240, Percent_Identity=30.8333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=239, Percent_Identity=30.1255230125523, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24667711, Length=274, Percent_Identity=36.8613138686131, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21356335, Length=274, Percent_Identity=36.8613138686131, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24667703, Length=274, Percent_Identity=36.8613138686131, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SHESA (A0KXS9)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_870004.1
- ProteinModelPortal:   A0KXS9
- SMR:   A0KXS9
- STRING:   A0KXS9
- GeneID:   4479604
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_2369
- NMPDR:   fig|94122.5.peg.2419
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   A0KXS9
- ProtClustDB:   CLSK906771
- BioCyc:   SSP94122:SHEWANA3_2369-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 29391; Mature: 29391

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTITAINAFNDNYIWVLRQDSQQAVYVVDPGDANVVLDYLHAQRLSLAGILITHHHRDH
CEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCC
TGGIAALTDHVKQTTGHDLAVYGPQSEDIQGINQPIEPTLSDSLTLPFIDAPVRILSVPG
CCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCCCCCCCCCCEECCEECCCEEEEECCC
HTAGHIAYLVDDALFCGDTLFSAGCGRLFEGTPSQMWQSLSLLAALPDETRVYCAHEYTL
CCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEC
SNLKFAQTVDTDNEALNAYVEEASTLRAQGKATIPSTIGLERAINPFLRPLSPTIVNSIK
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
NQFCDQDLTKADELTCFTLLRQWKDIF
HHHCCCHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTITAINAFNDNYIWVLRQDSQQAVYVVDPGDANVVLDYLHAQRLSLAGILITHHHRDH
CEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCC
TGGIAALTDHVKQTTGHDLAVYGPQSEDIQGINQPIEPTLSDSLTLPFIDAPVRILSVPG
CCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCCCCCCCCCCCEECCEECCCEEEEECCC
HTAGHIAYLVDDALFCGDTLFSAGCGRLFEGTPSQMWQSLSLLAALPDETRVYCAHEYTL
CCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEC
SNLKFAQTVDTDNEALNAYVEEASTLRAQGKATIPSTIGLERAINPFLRPLSPTIVNSIK
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
NQFCDQDLTKADELTCFTLLRQWKDIF
HHHCCCHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA