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The map label for this gene is mltD [H]

Identifier: 117920813

GI number: 117920813

Start: 2848212

End: 2849759

Strand: Direct

Name: mltD [H]

Synonym: Shewana3_2370

Alternate gene names: 117920813

Gene position: 2848212-2849759 (Clockwise)

Preceding gene: 117920812

Following gene: 117920817

Centisome position: 57.28

GC content: 48.84

Gene sequence:

>1548_bases
ATGAGAGTATCACTTTATGTTGTAGCAGGGGGATTTGCCCTGCTCACAGGTTGTCAGACTTTAGACAACCATCAGGTGAC
CAAACCTGAAGCTGAAACCGTCACAACTGAAAATACCGTCCCACAGTATTATCTCGCAACCGAACCTGAGTCGGCGCAAA
TTACTGATGTATGGGAGCGAATTCGTCAAGGAATGCAACTCCCCGTTCCTGACAAAAAATTAGTTAACTATTATCGCGAT
TGGTACATCAAGAACCCCAAACACCTCGAAGTGATTTCTGAGCGTGCGGCGCCTTACATGTATTTGATCGTCGAAGAGCT
TGAAAAGCGCCATCTCCCCATTGAGATCGCGTTGCTGCCGATTATCGAAAGCGCCTTCGATCCCAGTGCATCCTCAGCCA
GTGCCGCCTCAGGGCTTTGGCAATTTACAACCCCAATGGCCAGCCATTTTGGGTTAGAGATGAACTGGTGGTACGACGGC
CGTCGCGATGTGCCCGCCTCCACGGTGGCCGCGTTGGACATGCTCGAATACCTGTATGACAAAACCAATAATAATTGGCT
TTATGCGATTGCAGCTTACAACTCGGGCGAGTCTCGCGTCCTCAATGCGATTAAACGCAATGAAGATAAAGGCCTTAAAA
CCGATTTTTGGTCACTGGATCTGCCAAAAGAGACTGAGCGTTATGTGCCACAATTGTTGGCCCTCGCCGAAGTGATTAAA
CATGCCGACGAATACGGCATTACGCTAGCGCCAATTGACAACAGCCCTTCTATTGAAGTGGTTGATATCGATAGCCAAAT
TGACTTGGCCATGGCGGCTGGGCTCGCCAATATGACGACCCATGAGCTATTAAAACTCAATCCTGGATACAACCGCTGGG
CCACTGCACCGACGGGACCCCATACGCTGGTATTGCCGATTGATAAGTCAGAAGAGTTTAAAAAAGCACTGGCCGAAACC
GAGAGTGAAGCCCGTGTGAGTTGGCTCAGATACACCATTAAATCCGGTGACAGTATCGGTGCCATTGCCAAGAAACACCA
TACCACAGTCAATGCGATCCGTGCGGCTAATGGTATGAAAAATAACAATATCGTTGCGGGTAGACACCTAATTATTCCGG
TCTCTGCCGACGATAAACAGCTGTATGCCATGTCAACCAGCCAGAAGTTACCTAAAAAAGCGGCAACTTCAACCTCTGGC
AATAAGTTGACTTACCAAGTGAAATCCGGCGATACCCTATGGCAAATCGCGCAGAATCATAAGGTATCGGTGAAACAGCT
CACCGCTTGGAACCATTTAAACAAAGACAGTAAATTGCAGATGGGCCAAAAACTGACCATCGTTGCGCCGCCAACCCAAG
CGGAAGCGGAGCAGATTCGCACCGTCAGCTACAAGGTGAAATCTGGCGACTCCTTAGCGCGCATTGCCAGCAAGTTTAAA
GTGACGGTTGCCGAGCTGCTCGAGTGGAATAGCTTAACCTCATCGCAATACATTCAACCCGGCCAAGTGTTAAAGCTGGT
GGTGGATGAAAGCAATTTAAATGCGTGA

Upstream 100 bases:

>100_bases
TCTGTGTTGCCACTATTTACATAATAATCAATTTGTTAGCTCAATGAGGCTCGATGAGATTGTGCAGTGGCAATGCACTA
ACAGAGGACTATCGTTTTCC

Downstream 100 bases:

>100_bases
TGTTTAGTACTTAGCGACATAGCTGCTTAGATATTGAGTTAAGCCCATTAATCTGTACAAAAGCAGTGCAGGTTCGGCAA
GCATTAAACACTTAAGCCTT

Product: MltD domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRVSLYVVAGGFALLTGCQTLDNHQVTKPEAETVTTENTVPQYYLATEPESAQITDVWERIRQGMQLPVPDKKLVNYYRD
WYIKNPKHLEVISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDG
RRDVPASTVAALDMLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEDKGLKTDFWSLDLPKETERYVPQLLALAEVIK
HADEYGITLAPIDNSPSIEVVDIDSQIDLAMAAGLANMTTHELLKLNPGYNRWATAPTGPHTLVLPIDKSEEFKKALAET
ESEARVSWLRYTIKSGDSIGAIAKKHHTTVNAIRAANGMKNNNIVAGRHLIIPVSADDKQLYAMSTSQKLPKKAATSTSG
NKLTYQVKSGDTLWQIAQNHKVSVKQLTAWNHLNKDSKLQMGQKLTIVAPPTQAEAEQIRTVSYKVKSGDSLARIASKFK
VTVAELLEWNSLTSSQYIQPGQVLKLVVDESNLNA

Sequences:

>Translated_515_residues
MRVSLYVVAGGFALLTGCQTLDNHQVTKPEAETVTTENTVPQYYLATEPESAQITDVWERIRQGMQLPVPDKKLVNYYRD
WYIKNPKHLEVISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDG
RRDVPASTVAALDMLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEDKGLKTDFWSLDLPKETERYVPQLLALAEVIK
HADEYGITLAPIDNSPSIEVVDIDSQIDLAMAAGLANMTTHELLKLNPGYNRWATAPTGPHTLVLPIDKSEEFKKALAET
ESEARVSWLRYTIKSGDSIGAIAKKHHTTVNAIRAANGMKNNNIVAGRHLIIPVSADDKQLYAMSTSQKLPKKAATSTSG
NKLTYQVKSGDTLWQIAQNHKVSVKQLTAWNHLNKDSKLQMGQKLTIVAPPTQAEAEQIRTVSYKVKSGDSLARIASKFK
VTVAELLEWNSLTSSQYIQPGQVLKLVVDESNLNA
>Mature_515_residues
MRVSLYVVAGGFALLTGCQTLDNHQVTKPEAETVTTENTVPQYYLATEPESAQITDVWERIRQGMQLPVPDKKLVNYYRD
WYIKNPKHLEVISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDG
RRDVPASTVAALDMLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEDKGLKTDFWSLDLPKETERYVPQLLALAEVIK
HADEYGITLAPIDNSPSIEVVDIDSQIDLAMAAGLANMTTHELLKLNPGYNRWATAPTGPHTLVLPIDKSEEFKKALAET
ESEARVSWLRYTIKSGDSIGAIAKKHHTTVNAIRAANGMKNNNIVAGRHLIIPVSADDKQLYAMSTSQKLPKKAATSTSG
NKLTYQVKSGDTLWQIAQNHKVSVKQLTAWNHLNKDSKLQMGQKLTIVAPPTQAEAEQIRTVSYKVKSGDSLARIASKFK
VTVAELLEWNSLTSSQYIQPGQVLKLVVDESNLNA

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=509, Percent_Identity=33.2023575638507, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57414; Mature: 57414

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSLYVVAGGFALLTGCQTLDNHQVTKPEAETVTTENTVPQYYLATEPESAQITDVWER
CEEEEEEEECCCEEHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCHHHHHHHH
IRQGMQLPVPDKKLVNYYRDWYIKNPKHLEVISERAAPYMYLIVEELEKRHLPIEIALLP
HHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEEEEEEH
IIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDGRRDVPASTVAALDMLEYLYD
HHHHHCCCCCCCCHHHCCCEEECCHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KTNNNWLYAIAAYNSGESRVLNAIKRNEDKGLKTDFWSLDLPKETERYVPQLLALAEVIK
CCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HADEYGITLAPIDNSPSIEVVDIDSQIDLAMAAGLANMTTHELLKLNPGYNRWATAPTGP
HHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCHHHCCHHHHEEECCCCCCCCCCCCCC
HTLVLPIDKSEEFKKALAETESEARVSWLRYTIKSGDSIGAIAKKHHTTVNAIRAANGMK
CEEEEECCCCHHHHHHHHCCCHHHHHHHHEEEECCCCCHHHHHHHHCHHHHHHHHHCCCC
NNNIVAGRHLIIPVSADDKQLYAMSTSQKLPKKAATSTSGNKLTYQVKSGDTLWQIAQNH
CCCEEECCEEEEEECCCCCEEEEECCHHHCCHHHCCCCCCCEEEEEECCCCEEEHHHCCC
KVSVKQLTAWNHLNKDSKLQMGQKLTIVAPPTQAEAEQIRTVSYKVKSGDSLARIASKFK
CCCHHHHHHHHHCCCCCHHCCCCEEEEEECCCCHHHHHEEEEEEEECCCCHHHHHHHHHH
VTVAELLEWNSLTSSQYIQPGQVLKLVVDESNLNA
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRVSLYVVAGGFALLTGCQTLDNHQVTKPEAETVTTENTVPQYYLATEPESAQITDVWER
CEEEEEEEECCCEEHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCHHHHHHHH
IRQGMQLPVPDKKLVNYYRDWYIKNPKHLEVISERAAPYMYLIVEELEKRHLPIEIALLP
HHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEEEEEEH
IIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDGRRDVPASTVAALDMLEYLYD
HHHHHCCCCCCCCHHHCCCEEECCHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KTNNNWLYAIAAYNSGESRVLNAIKRNEDKGLKTDFWSLDLPKETERYVPQLLALAEVIK
CCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HADEYGITLAPIDNSPSIEVVDIDSQIDLAMAAGLANMTTHELLKLNPGYNRWATAPTGP
HHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCHHHCCHHHHEEECCCCCCCCCCCCCC
HTLVLPIDKSEEFKKALAETESEARVSWLRYTIKSGDSIGAIAKKHHTTVNAIRAANGMK
CEEEEECCCCHHHHHHHHCCCHHHHHHHHEEEECCCCCHHHHHHHHCHHHHHHHHHCCCC
NNNIVAGRHLIIPVSADDKQLYAMSTSQKLPKKAATSTSGNKLTYQVKSGDTLWQIAQNH
CCCEEECCEEEEEECCCCCEEEEECCHHHCCHHHCCCCCCCEEEEEECCCCEEEHHHCCC
KVSVKQLTAWNHLNKDSKLQMGQKLTIVAPPTQAEAEQIRTVSYKVKSGDSLARIASKFK
CCCHHHHHHHHHCCCCCHHCCCCEEEEEECCCCHHHHHEEEEEEEECCCCHHHHHHHHHH
VTVAELLEWNSLTSSQYIQPGQVLKLVVDESNLNA
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]