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The map label for this gene is rnhA [H]

Identifier: 117920809

GI number: 117920809

Start: 2844810

End: 2845286

Strand: Direct

Name: rnhA [H]

Synonym: Shewana3_2366

Alternate gene names: 117920809

Gene position: 2844810-2845286 (Clockwise)

Preceding gene: 117920806

Following gene: 117920812

Centisome position: 57.21

GC content: 51.15

Gene sequence:

>477_bases
ATGACTGAACTAAAACTGATCCACATCTTCACCGATGGCTCTTGCTTAGGCAACCCTGGCCCCGGTGGCTATGGCATTGT
AATGAATTATAAGGGCCATACAAAGGAGATGTCCGATGGTTTTGCCTTGACGACCAATAATCGCATGGAATTATTAGCCC
CCATCGTCGCCCTCGAGGCGTTAAAAGAGCCTTGCAAGGTCATTTTAACCAGCGATAGCCAATATATGCGCCAAGGGATC
ACCACCTGGATCCATGGCTGGAAGAAAAAAGGCTGGATGACCTCGAATCGTACGCCAGTGAAAAATGTTGATCTCTGGAA
ACGTTTAGACAAAGCCGCCCAACTCCATCAAATTGATTGGCGTTGGGTGAAAGGCCACGCCGGACACCCTGAAAACGAGC
GCTGCGATCAACTCGCCCGCGCCGCCGCTGAAGCGAGCCCTACCCAAGTCGATGAAGGCTATCAGCCTGAGTCCTAA

Upstream 100 bases:

>100_bases
CTGACGGCTTGCGTTTGAGATAATATTCATACTTTTATGCGCTTCTTATGACATTTTTTGGTTATACTCGGCCGGTATTT
ACGCTGCGGATAACCATTTG

Downstream 100 bases:

>100_bases
AACTGGCCCTCATTTCGGCGCTAAACGTTGCGCCGATCACTCCCGAGATAAATGGAAAAAATTACCGCAAAACTGCACAA
AGGCTAGGCCTAATGACGTG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HI [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIVALEALKEPCKVILTSDSQYMRQGI
TTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKAAQLHQIDWRWVKGHAGHPENERCDQLARAAAEASPTQVDEGYQPES

Sequences:

>Translated_158_residues
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIVALEALKEPCKVILTSDSQYMRQGI
TTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKAAQLHQIDWRWVKGHAGHPENERCDQLARAAAEASPTQVDEGYQPES
>Mature_157_residues
TELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIVALEALKEPCKVILTSDSQYMRQGIT
TWIHGWKKKGWMTSNRTPVKNVDLWKRLDKAAQLHQIDWRWVKGHAGHPENERCDQLARAAAEASPTQVDEGYQPES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=146, Percent_Identity=34.9315068493151, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1786408, Length=154, Percent_Identity=70.1298701298701, Blast_Score=232, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=36.1702127659575, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137476, Length=141, Percent_Identity=36.8794326241135, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552509, Length=141, Percent_Identity=36.8794326241135, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17761; Mature: 17630

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIVALEA
CCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCKVILTSDSQYMRQGITTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKAAQLHQIDW
HHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
RWVKGHAGHPENERCDQLARAAAEASPTQVDEGYQPES
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TELKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIVALEA
CCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCKVILTSDSQYMRQGITTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKAAQLHQIDW
HHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCE
RWVKGHAGHPENERCDQLARAAAEASPTQVDEGYQPES
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]