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The map label for this gene is nrfC [H]

Identifier: 117920783

GI number: 117920783

Start: 2814516

End: 2815220

Strand: Reverse

Name: nrfC [H]

Synonym: Shewana3_2340

Alternate gene names: 117920783

Gene position: 2815220-2814516 (Counterclockwise)

Preceding gene: 117920784

Following gene: 117920780

Centisome position: 56.62

GC content: 45.53

Gene sequence:

>705_bases
ATGAGATTAGGAATGGTGATTGACCTACAAAAATGTGTAGGGTGTGGCGGCTGTAGCTTAGCCTGTAAAACAGAAAACAA
CACGAACGACGGTATTCACTGGTCGCATCATATTGCGACCACTGAGGGTACGTTCCCTGACGTTAAATACACATATATAC
CGACCCTGTGTAACCATTGTGATGATGCACCATGTGTTAAAGTGTGTCCTACAGGAGCGATGCACAAAGATAAACGCGGT
TTAACGCTGCAAAACAATGATGAATGCATTGGTTGCAAGAAGTGTATGAATGCTTGCCCATACGGCGTGATTAGTTTTAA
TGCGGCAACACCGCACCGTCGTTGGCAGGATGACTCAGAGGTGGTCGCGAACGGAACTGTATCTCCACTTATGCTACTAA
AGCGTACAGGGGCTACGGCAACACCTAATGAAAACCCAGAGCGGGGTGATACCTATCCGATGATACGGCCAAAGCGTACT
ACAGAGAAATGTACTTTCTGTGATCATAGGCTTGATAAGGGGTTGAATCCTGCCTGTGTTGACGCTTGTCCATCTGAAGC
ACGGGTAATTGGTGATTTAGATGATCCGCAGAGCAAGGTATCCCAGTTGATTAAATTGCATAAACCTATGCAGTTGAAAC
CTGAAGCGGGAACGGGACCAAGGGTATTTTATATTCGCAGTTTTGGTGTTAAAACCGCTTATTAA

Upstream 100 bases:

>100_bases
ATAATGATTTGATTGCAGACAGATACGATCGCTTAAGTGGTGCGTCGGCATTTTATGGTCATATCCGTGTTCGTGTTGAG
AAAGTGTGAGGTAACCCACT

Downstream 100 bases:

>100_bases
TCATAGAGGTTAATTTGTTAGTGAAAGCCACAGATGAGATTCTGTGGCTTTTTACTTTATACGCGCCCTATCAATCACTA
AACATCAGCGAATCTAATAC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHCDDAPCVKVCPTGAMHKDKRG
LTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSEVVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRT
TEKCTFCDHRLDKGLNPACVDACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY

Sequences:

>Translated_234_residues
MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHCDDAPCVKVCPTGAMHKDKRG
LTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSEVVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRT
TEKCTFCDHRLDKGLNPACVDACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY
>Mature_234_residues
MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHCDDAPCVKVCPTGAMHKDKRG
LTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSEVVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRT
TEKCTFCDHRLDKGLNPACVDACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=228, Percent_Identity=33.7719298245614, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI226510944, Length=232, Percent_Identity=28.8793103448276, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=236, Percent_Identity=28.3898305084746, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787122, Length=236, Percent_Identity=27.9661016949153, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789067, Length=208, Percent_Identity=31.7307692307692, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789370, Length=229, Percent_Identity=27.0742358078603, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788811, Length=187, Percent_Identity=28.3422459893048, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082114, Length=197, Percent_Identity=28.9340101522843, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082179, Length=192, Percent_Identity=24.4791666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367245, Length=207, Percent_Identity=26.5700483091787, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=171, Percent_Identity=23.9766081871345, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789079, Length=195, Percent_Identity=28.7179487179487, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082180, Length=104, Percent_Identity=31.7307692307692, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1787478, Length=150, Percent_Identity=26, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25733; Mature: 25733

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHC
CCCCEEEEHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEHHHHHCCC
DDAPCVKVCPTGAMHKDKRGLTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSE
CCCCEEEECCCCCCCCCCCCCEECCCCCEECHHHHHHHCCCCEEEECCCCCCCCCCCCCC
VVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRTTEKCTFCDHRLDKGLNPACV
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
DACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY
HCCCCCCEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHC
CCCCEEEEHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEHHHHHCCC
DDAPCVKVCPTGAMHKDKRGLTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSE
CCCCEEEECCCCCCCCCCCCCEECCCCCEECHHHHHHHCCCCEEEECCCCCCCCCCCCCC
VVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRTTEKCTFCDHRLDKGLNPACV
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
DACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY
HCCCCCCEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]