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The map label for this gene is ddl [H]

Identifier: 117920750

GI number: 117920750

Start: 2772189

End: 2773199

Strand: Direct

Name: ddl [H]

Synonym: Shewana3_2307

Alternate gene names: 117920750

Gene position: 2772189-2773199 (Clockwise)

Preceding gene: 117920749

Following gene: 117920755

Centisome position: 55.75

GC content: 47.48

Gene sequence:

>1011_bases
ATGTCAAAAATCAATTTACTTTTGCTGTGCGGCGGTGGAAGTGCGGAACACGATATTTCCCTACTGTCGGCTAATTATTT
TGAAACCTCATTAGCAAAATCCGAGCAGTTTAATGTACTGCGCGTGGTACTGGATAAATTTGGCCAATACCAAACCGCCG
CAGGCGATGACTGTGAACTGACCAATAACCGCGAAATTCGCTTCCGTGATGAGACCAAGCCCGCATGGCCTGTGGATTAT
GTCATTCCTTGTATTCACGGCTATCCGGGGGAAACGGGCGACATTCAGTCTTACTTTAATCTTATCCAACTGCCCTACTT
TGGTTGCGAATCCGAAGCCAGCAGCAACTGCTTTAACAAGATCACCGCTAAGATGTGGTTCAGCGCCTTAGGCATTCCTA
ACACACCTTATATCTTCTTAAATCAATACGATGATGACGCGATTGCCCAAACCCAAGCAGCGCTCGAAAACTGGGGGTCG
ATTTTCGTTAAAGCCGCATCCCAAGGCTCATCGGTCGGTTGCTATAAAGTCGATGACAGCAGCAAAGTGGCTGACGTGTT
AAAAGATGCCTTTGGCTACGCGCCCTATGTGATCGTCGAGAAAACCATTAAGGCCCGTGAGCTGGAAGTCGCGGTCTATG
AGTATCAAGGCGAAGTGGTTGCGACCCTGCCGGGCGAAATCATCTGTGATAGCAATACCTTCTACACCTTCGATGAGAAA
TACGCCAAGAGCAGTAAGGCACGCACCGATGTGGTCGCGCAAAATGTGCCAACTGACATTAGTGAGCAGATCCGCGCCTA
TGCGATTAAAGCCTTTAAGGGCATGAAGCTGCGCCATCTGTCACGCATCGACTTTTTCTTAACCCAAGATAATGAAATTC
TACTCAACGAAATCAATACCTTCCCGGGTTCGACGCCGATTTCAATGTTCCCTAAGATGTTGCAAAACCATGGTCATGAT
TTTACCGAATACTTAAGTTTGGTGATTAACGGCCAGTTAGCGGCTAAGTGA

Upstream 100 bases:

>100_bases
CGCAGATAACAGCCCTTGTTGCAGGAAGTGACGAAGTTGTCGTTAGTAAGCGAGTTGCAAAAGGCAACTCAACAGCATTT
TGCTCAATCGAGGAATCATA

Downstream 100 bases:

>100_bases
TAGACCGAGTGATAGGCTAAATAAACTGAAAAGCGCGCCTATATAGCGCGCTTCTTTTTTGAGGTTGGTTTGAACTCATT
ATGAATGGTGATGAGCCGCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSKINLLLLCGGGSAEHDISLLSANYFETSLAKSEQFNVLRVVLDKFGQYQTAAGDDCELTNNREIRFRDETKPAWPVDY
VIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLNQYDDDAIAQTQAALENWGS
IFVKAASQGSSVGCYKVDDSSKVADVLKDAFGYAPYVIVEKTIKARELEVAVYEYQGEVVATLPGEIICDSNTFYTFDEK
YAKSSKARTDVVAQNVPTDISEQIRAYAIKAFKGMKLRHLSRIDFFLTQDNEILLNEINTFPGSTPISMFPKMLQNHGHD
FTEYLSLVINGQLAAK

Sequences:

>Translated_336_residues
MSKINLLLLCGGGSAEHDISLLSANYFETSLAKSEQFNVLRVVLDKFGQYQTAAGDDCELTNNREIRFRDETKPAWPVDY
VIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLNQYDDDAIAQTQAALENWGS
IFVKAASQGSSVGCYKVDDSSKVADVLKDAFGYAPYVIVEKTIKARELEVAVYEYQGEVVATLPGEIICDSNTFYTFDEK
YAKSSKARTDVVAQNVPTDISEQIRAYAIKAFKGMKLRHLSRIDFFLTQDNEILLNEINTFPGSTPISMFPKMLQNHGHD
FTEYLSLVINGQLAAK
>Mature_335_residues
SKINLLLLCGGGSAEHDISLLSANYFETSLAKSEQFNVLRVVLDKFGQYQTAAGDDCELTNNREIRFRDETKPAWPVDYV
IPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLNQYDDDAIAQTQAALENWGSI
FVKAASQGSSVGCYKVDDSSKVADVLKDAFGYAPYVIVEKTIKARELEVAVYEYQGEVVATLPGEIICDSNTFYTFDEKY
AKSSKARTDVVAQNVPTDISEQIRAYAIKAFKGMKLRHLSRIDFFLTQDNEILLNEINTFPGSTPISMFPKMLQNHGHDF
TEYLSLVINGQLAAK

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.3333333333333, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786280, Length=326, Percent_Identity=26.6871165644172, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 37500; Mature: 37368

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKINLLLLCGGGSAEHDISLLSANYFETSLAKSEQFNVLRVVLDKFGQYQTAAGDDCEL
CCCEEEEEEECCCCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEE
TNNREIRFRDETKPAWPVDYVIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNK
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ITAKMWFSALGIPNTPYIFLNQYDDDAIAQTQAALENWGSIFVKAASQGSSVGCYKVDDS
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCC
SKVADVLKDAFGYAPYVIVEKTIKARELEVAVYEYQGEVVATLPGEIICDSNTFYTFDEK
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCEEEECCCEEEECCCCEEECCHH
YAKSSKARTDVVAQNVPTDISEQIRAYAIKAFKGMKLRHLSRIDFFLTQDNEILLNEINT
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHHC
FPGSTPISMFPKMLQNHGHDFTEYLSLVINGQLAAK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKINLLLLCGGGSAEHDISLLSANYFETSLAKSEQFNVLRVVLDKFGQYQTAAGDDCEL
CCEEEEEEECCCCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEE
TNNREIRFRDETKPAWPVDYVIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNK
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ITAKMWFSALGIPNTPYIFLNQYDDDAIAQTQAALENWGSIFVKAASQGSSVGCYKVDDS
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCC
SKVADVLKDAFGYAPYVIVEKTIKARELEVAVYEYQGEVVATLPGEIICDSNTFYTFDEK
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCCEEEECCCEEEECCCCEEECCHH
YAKSSKARTDVVAQNVPTDISEQIRAYAIKAFKGMKLRHLSRIDFFLTQDNEILLNEINT
HHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHHC
FPGSTPISMFPKMLQNHGHDFTEYLSLVINGQLAAK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA