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The map label for this gene is ptrB [C]

Identifier: 117920672

GI number: 117920672

Start: 2679951

End: 2682044

Strand: Reverse

Name: ptrB [C]

Synonym: Shewana3_2229

Alternate gene names: 117920672

Gene position: 2682044-2679951 (Counterclockwise)

Preceding gene: 117920673

Following gene: 117920671

Centisome position: 53.94

GC content: 49.04

Gene sequence:

>2094_bases
ATGAATCGTAAATTACTGCCTTGGTGCATTGCAGGGGTACTCACTATGAGTGGTCAACTCCACGCCGAAGAAGACAAATA
TATTTGGCTCGAAGAGGTTGAAGGCGCAAAGCCGATGGAGTGGGTTAAGGCCCAAAATGCTACTTCTGCCGCCGAAATAA
AAGCCTTCAAAGGGTTCGATACTTTAGTTGCCAACAGCCTCGCTATCCTCAATGACAAAGAGCGTATTCCTTACGCCACC
CATATTGGCGATAAGCTGTATAACTTTTGGAAGGACGATACCCATGTGCGCGGGATTTACCGTCGCACCACCATGGAAGA
ATACGCCAAGGCGGATCCAAAGTGGGAAACCGTGCTCGATGTCGATGCCTTAGGTAAAACCGAAGCGGTCAACTGGGTGT
TCAAAGGCATTGATTGCCAGTATCCACAGAATCAGCGCTGTTTTGTGTCCTTATCCCGTGGCGGCGCCGATGCGGTCGAA
GTGCGTGAATTTGATTTAAGCACCAAAGACTTTGTGCCCGCGAAAGATAAACCTTTCTTCTTAAAAGAAGCGAAATCTAG
CCTTAGTTGGATTGATACCGATCAGGCCTTTGTCGGTACCGATTTTGGCGATGACCAAAGCATGACGGACTCGGGTTATC
CCCGCGTAGTGAAGTTATGGCAACGTGGTACGCCGCTTGAGCAAGCGAAAACCATTTTCAGTGGCGACAAAACTTCGGTC
GCGGTATCGGGTTGGGTGATGTTTGACGACAAAACTCCGCTGAGCCTAGTTACTGAGGCGCATACCTTTTACACCGCCAC
TCAATATGTTTACCAAGACGGCAAACTGACTAAGCTGCCGCTCCCGCAAGATGCCGAGATCAAAGGCTATTTCCAAGGCA
AATTGTTTATTGAGCTCAAGAGTGAGTTAGCAACCCCTGCGACGACATTCAGCCAAGGCGCCGTGGTGTATGCCAATGTG
GCGGATTTAATCGCCCAAAAAGCATCCTTTACTGAATTTGTCAGCCCAACGCCGACCGCATCAATCGCCCAGTTAAGTTT
CAGTAAGAGCGCGATTTTTGTAAATTGGCTCGATAACGTGAAGAGTAAACTGGTTCGCTACGAGCAAGACGCGAAGGGCG
CATGGCAAAGCACGCCCGTCCCCTTTGAGGCCAATGGCGCGCTAACCGTGATGGATATGGAGCGCGACAGTGATGATTTC
TTTGTTAATTACACGAGCTTTCTTGAGCCATCGACCCTGTATACCGTCAATGCTAAGGCGCTAAAACCGCAAAAAATTAA
AGGTATGCCTCAGCAATTTGCAGCGGATAAATTTAAAACTGAGCAGTATTTTGCAACCTCAAAGGATGGCACCAAAGTGC
CGTATTTTGTGGTGATGGCAAAGGATCTTAAGCTCGATGGCAGCAATCCCACTCTGCTCTATGGTTATGGTGGTTTTGAA
GTGTCACTGCGCCCAGCCTATTCGGCAACCATTGGTAAAAACTGGTTAGAGCAGGGCGGCGTGTATGTGCTGGCCAATAT
CCGTGGTGGCGGCGAGTACGGCCCTGCGTGGCATCAAGCGGCACTTAAGCAGAATCGTCATAAGGCCTATGAGGACTTTG
AGGCTATCGCCGAGGATTTAATCGCCCGTAAAATTACCTCGAGCAAGCATTTAGGCATTCAAGGTGGCAGTAATGGTGGT
TTGCTGATGGGCGCCGCTTTTACCCGTCGTCCCGATCTCTACAATGCAGTGGTCTGCCAAGTGCCTTTACTCGACATGTA
CCGCTTCAATAAGTTACTGGCCGGCGCAAGTTGGATGGGGGAATACGGTAATCCCGATGTTCCTGAAGAATGGGCCTACA
TTAAAACCTATTCGCCATACCATAATCTGCACAAGGACACCCACTATCCTAAGGTGTTCTTCACTACCTCAACCCGTGAT
GACAGGGTACACCCAGGCCATGCGCGTAAGATGGTAGCCAAGATGAAGGACATGGGCATCGATGTGCTTTACTATGAAAA
TATCGAAGGTGGACACGCAGGCGCTGCAGATAATAATCAAGCTGCGGAACTAAATTCGATGGCGTTTGCCTACTTATTAC
AGCAGTTAAAATAA

Upstream 100 bases:

>100_bases
AAAGGTTGAACTGGAGGTCAATTAAGGTAATGTAGGGCAGAAATCAAGATGGACTCAATTTAAGGGCTCTGTTCAAGGAC
TCTGTCTAAAAAGGAATAAC

Downstream 100 bases:

>100_bases
TCGTGATGCGTGACAGAAAATATTAACAATTCGCGATTAATACCGAGCAAGTCCCTATAGGTTGGGGAGCAAAAAACGGT
CGAATGCCTTGAGGTTCGGC

Product: peptidase S9 prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNRKLLPWCIAGVLTMSGQLHAEEDKYIWLEEVEGAKPMEWVKAQNATSAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKADPKWETVLDVDALGKTEAVNWVFKGIDCQYPQNQRCFVSLSRGGADAVE
VREFDLSTKDFVPAKDKPFFLKEAKSSLSWIDTDQAFVGTDFGDDQSMTDSGYPRVVKLWQRGTPLEQAKTIFSGDKTSV
AVSGWVMFDDKTPLSLVTEAHTFYTATQYVYQDGKLTKLPLPQDAEIKGYFQGKLFIELKSELATPATTFSQGAVVYANV
ADLIAQKASFTEFVSPTPTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDAKGAWQSTPVPFEANGALTVMDMERDSDDF
FVNYTSFLEPSTLYTVNAKALKPQKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGSNPTLLYGYGGFE
VSLRPAYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMYRFNKLLAGASWMGEYGNPDVPEEWAYIKTYSPYHNLHKDTHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNSMAFAYLLQQLK

Sequences:

>Translated_697_residues
MNRKLLPWCIAGVLTMSGQLHAEEDKYIWLEEVEGAKPMEWVKAQNATSAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKADPKWETVLDVDALGKTEAVNWVFKGIDCQYPQNQRCFVSLSRGGADAVE
VREFDLSTKDFVPAKDKPFFLKEAKSSLSWIDTDQAFVGTDFGDDQSMTDSGYPRVVKLWQRGTPLEQAKTIFSGDKTSV
AVSGWVMFDDKTPLSLVTEAHTFYTATQYVYQDGKLTKLPLPQDAEIKGYFQGKLFIELKSELATPATTFSQGAVVYANV
ADLIAQKASFTEFVSPTPTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDAKGAWQSTPVPFEANGALTVMDMERDSDDF
FVNYTSFLEPSTLYTVNAKALKPQKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGSNPTLLYGYGGFE
VSLRPAYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMYRFNKLLAGASWMGEYGNPDVPEEWAYIKTYSPYHNLHKDTHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNSMAFAYLLQQLK
>Mature_697_residues
MNRKLLPWCIAGVLTMSGQLHAEEDKYIWLEEVEGAKPMEWVKAQNATSAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKADPKWETVLDVDALGKTEAVNWVFKGIDCQYPQNQRCFVSLSRGGADAVE
VREFDLSTKDFVPAKDKPFFLKEAKSSLSWIDTDQAFVGTDFGDDQSMTDSGYPRVVKLWQRGTPLEQAKTIFSGDKTSV
AVSGWVMFDDKTPLSLVTEAHTFYTATQYVYQDGKLTKLPLPQDAEIKGYFQGKLFIELKSELATPATTFSQGAVVYANV
ADLIAQKASFTEFVSPTPTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDAKGAWQSTPVPFEANGALTVMDMERDSDDF
FVNYTSFLEPSTLYTVNAKALKPQKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGSNPTLLYGYGGFE
VSLRPAYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMYRFNKLLAGASWMGEYGNPDVPEEWAYIKTYSPYHNLHKDTHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNSMAFAYLLQQLK

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=359, Percent_Identity=33.983286908078, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI284172438, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI284172431, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI108860686, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI284172420, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI284172413, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI70778815, Length=209, Percent_Identity=29.6650717703349, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI108860692, Length=258, Percent_Identity=27.1317829457364, Blast_Score=87, Evalue=8e-17,
Organism=Escherichia coli, GI1788150, Length=357, Percent_Identity=26.890756302521, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24583414, Length=334, Percent_Identity=37.125748502994, Blast_Score=213, Evalue=5e-55,
Organism=Drosophila melanogaster, GI221510989, Length=281, Percent_Identity=36.2989323843416, Blast_Score=192, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 77986; Mature: 77986

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKLLPWCIAGVLTMSGQLHAEEDKYIWLEEVEGAKPMEWVKAQNATSAAEIKAFKGFD
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHCCHH
TLVANSLAILNDKERIPYATHIGDKLYNFWKDDTHVRGIYRRTTMEEYAKADPKWETVLD
HHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
VDALGKTEAVNWVFKGIDCQYPQNQRCFVSLSRGGADAVEVREFDLSTKDFVPAKDKPFF
HHHCCCCHHEEHEEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCHH
LKEAKSSLSWIDTDQAFVGTDFGDDQSMTDSGYPRVVKLWQRGTPLEQAKTIFSGDKTSV
HHHHHCCCCEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEE
AVSGWVMFDDKTPLSLVTEAHTFYTATQYVYQDGKLTKLPLPQDAEIKGYFQGKLFIELK
EEEEEEEECCCCCHHHHHHHHHHEEEEEEEEECCCEEECCCCCCCCEEEEEEEEEEEEEH
SELATPATTFSQGAVVYANVADLIAQKASFTEFVSPTPTASIAQLSFSKSAIFVNWLDNV
HHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCEEEEEHHHHH
KSKLVRYEQDAKGAWQSTPVPFEANGALTVMDMERDSDDFFVNYTSFLEPSTLYTVNAKA
HHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCCCEEEEEHHHCCCCEEEEEECCC
LKPQKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGSNPTLLYGYGGFE
CCCHHHCCCHHHHHHHHHCCHHEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEE
VSLRPAYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDL
EEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IARKITSSKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMYRFNKLLAGASWMG
HHHHHCCCCCEEEECCCCCCEEEEEHHCCCCCHHCEEEEECCHHHHHHHHHHHHCCHHCC
EYGNPDVPEEWAYIKTYSPYHNLHKDTHYPKVFFTTSTRDDRVHPGHARKMVAKMKDMGI
CCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
DVLYYENIEGGHAGAADNNQAAELNSMAFAYLLQQLK
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRKLLPWCIAGVLTMSGQLHAEEDKYIWLEEVEGAKPMEWVKAQNATSAAEIKAFKGFD
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHCCHH
TLVANSLAILNDKERIPYATHIGDKLYNFWKDDTHVRGIYRRTTMEEYAKADPKWETVLD
HHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
VDALGKTEAVNWVFKGIDCQYPQNQRCFVSLSRGGADAVEVREFDLSTKDFVPAKDKPFF
HHHCCCCHHEEHEEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCHH
LKEAKSSLSWIDTDQAFVGTDFGDDQSMTDSGYPRVVKLWQRGTPLEQAKTIFSGDKTSV
HHHHHCCCCEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEE
AVSGWVMFDDKTPLSLVTEAHTFYTATQYVYQDGKLTKLPLPQDAEIKGYFQGKLFIELK
EEEEEEEECCCCCHHHHHHHHHHEEEEEEEEECCCEEECCCCCCCCEEEEEEEEEEEEEH
SELATPATTFSQGAVVYANVADLIAQKASFTEFVSPTPTASIAQLSFSKSAIFVNWLDNV
HHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCEEEEEHHHHH
KSKLVRYEQDAKGAWQSTPVPFEANGALTVMDMERDSDDFFVNYTSFLEPSTLYTVNAKA
HHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCCCEEEEEHHHCCCCEEEEEECCC
LKPQKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGSNPTLLYGYGGFE
CCCHHHCCCHHHHHHHHHCCHHEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEE
VSLRPAYSATIGKNWLEQGGVYVLANIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDL
EEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IARKITSSKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMYRFNKLLAGASWMG
HHHHHCCCCCEEEECCCCCCEEEEEHHCCCCCHHCEEEEECCHHHHHHHHHHHHCCHHCC
EYGNPDVPEEWAYIKTYSPYHNLHKDTHYPKVFFTTSTRDDRVHPGHARKMVAKMKDMGI
CCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
DVLYYENIEGGHAGAADNNQAAELNSMAFAYLLQQLK
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]