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The map label for this gene is eptA [H]

Identifier: 117920671

GI number: 117920671

Start: 2678067

End: 2679692

Strand: Reverse

Name: eptA [H]

Synonym: Shewana3_2228

Alternate gene names: 117920671

Gene position: 2679692-2678067 (Counterclockwise)

Preceding gene: 117920672

Following gene: 117920670

Centisome position: 53.89

GC content: 46.68

Gene sequence:

>1626_bases
GTGCTGACGAGATTTAAAACTCTAACCGTGAACCGCTTCACCTTTATCACCGCATTATTTTACGTTTGCGTTTTTAATAT
CCCACTCTTTGAAGTCATCAAGAAAGGCATTGAAAAACAGCCCGATGTGGATCCGATTTTTATCGCATCCATGCCGCTGT
TTTTAACCTTTGCACTGAGTTTTGTGTTTTCACTGTTTACGGTGAAATACCTAGTAAAACCCTTTTTCATCATTCTCACG
CTGCTGTCATCGAGCGTGTTTTTTGCGGCGCTCAAATACAATGTCGTGTTCGACTACGGCATGATTGAAAACACCTTTCA
AACCAACAGCGCCGAAGCCTTGATGTATGTGAACTTTGCTTCCATCCTCAACATGCTGCTCACGGGGATTTTACCCGCCT
ATTTGATTTACAAAGCCAATATCCAATACAAGCCATTTTTTAAAGAGTTGCTGCATAAAGTCTTGTTTATGCTGGCGATG
TTGGCAGGGCTTGGGATTATCGCCTTCTTCTATTTCCAAGATTACGCCGCCTTTGGCCGCAATAACGATGAGATCAAGCG
TTATATTGTGCCGACCTATTTTATTGGCGCGGCCGCTAAGTACATCAATGTGCACTATTTGCAAACGCCGATTGAATACC
AACAACTGGGTTTGGATGCCAAAAATGTTACAACCAATGCCAACGGTAAACCTAACCTGTTAGTGCTGGTGGTGGGCGAG
ACTGCTCGTTCAATGAACTATCAATACTATGGTTATGATAAACCGACCAACGCTTACACAGAGGGCCAAGGCTTGGTCGC
CTTTAAAGATACCCATTCCTGCGGCACGGCCACTGCGGTCTCGCTACCTTGCATGTTCTCGCGGATGGGACGTGAGGATT
ACGATTCTCGCCGCGCCTATGCCCAAGACACGGCCATCGATGTGTTAAATCACGGCGGGATTAAAGTGCAGTGGTTTGAT
AATGACTCGGGTTGTAAAGGGGTGTGCGATCAGGTTGAAAACATCACCATTGACTTGAATAGCGATCCAGAACTCTGCTC
GGGACAATATTGTTTCGACCAAGTGCTGCTCAATAAACTCGACGAAGCCTTAGCCAATGCGGAGCAGAAAGACACTGTGA
TAGCCCTGCATGTGATTGGCAGCCATGGGCCGACGTACTTCCTACGCTATCCGCCAGAACACCGTAAATTTACGCCGGAT
TGCCCGCGTAGCGACATTCAAAACTGCAGCGCCGAAGAGCTGATGAACACCTATGACAACACGATTTTGTATACCGATTA
CATTATCAGTGAAGTGGTCAATAAGCTTAAACAGCAACAGACTAAGTTTGATACCGCCATGTTCTATGTGTCGGATCACG
GCGAATCTCTGGGTGAGAAAGGCATGTATTTGCATGGCGCGCCGTACGGTATTGCGCCGATTGAACAGACCAGTATTCCT
ATGCTGGCCTGGGTGTCGGAGGATTTCAGCCAAGACAACCATTTGAATATGGCGTGTTTAGCCAAAGAGGCCGAGAAGGG
CGGCTTCTCCCACGACAATCTGTTCGACAGTCTGCTGGGATTAATGAATGTTCAGACCCAAGTGTATCAATCTAAGTTAG
ATATTTTTGCTCGCTGCCGCGGTTAA

Upstream 100 bases:

>100_bases
CAAGTTCAGTTGCTAAGATGACATTCATTTTTAGGCATAAGATTCCCTTAAGAAAACTAAGCTAGTTTCGGCCCACTATT
TATTTAAGAGAGCATTACCA

Downstream 100 bases:

>100_bases
GTTCAGCGTGCAGGTGAATACATAAAGGAGGATGGCAACATCCTCCTTTATTTTTTTGCGCCTTATCATAATAATTATTG
TGTCCCGTATCCAAATCGAA

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLTRFKTLTVNRFTFITALFYVCVFNIPLFEVIKKGIEKQPDVDPIFIASMPLFLTFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAALKYNVVFDYGMIENTFQTNSAEALMYVNFASILNMLLTGILPAYLIYKANIQYKPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDEIKRYIVPTYFIGAAAKYINVHYLQTPIEYQQLGLDAKNVTTNANGKPNLLVLVVGE
TARSMNYQYYGYDKPTNAYTEGQGLVAFKDTHSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAIDVLNHGGIKVQWFD
NDSGCKGVCDQVENITIDLNSDPELCSGQYCFDQVLLNKLDEALANAEQKDTVIALHVIGSHGPTYFLRYPPEHRKFTPD
CPRSDIQNCSAEELMNTYDNTILYTDYIISEVVNKLKQQQTKFDTAMFYVSDHGESLGEKGMYLHGAPYGIAPIEQTSIP
MLAWVSEDFSQDNHLNMACLAKEAEKGGFSHDNLFDSLLGLMNVQTQVYQSKLDIFARCRG

Sequences:

>Translated_541_residues
MLTRFKTLTVNRFTFITALFYVCVFNIPLFEVIKKGIEKQPDVDPIFIASMPLFLTFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAALKYNVVFDYGMIENTFQTNSAEALMYVNFASILNMLLTGILPAYLIYKANIQYKPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDEIKRYIVPTYFIGAAAKYINVHYLQTPIEYQQLGLDAKNVTTNANGKPNLLVLVVGE
TARSMNYQYYGYDKPTNAYTEGQGLVAFKDTHSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAIDVLNHGGIKVQWFD
NDSGCKGVCDQVENITIDLNSDPELCSGQYCFDQVLLNKLDEALANAEQKDTVIALHVIGSHGPTYFLRYPPEHRKFTPD
CPRSDIQNCSAEELMNTYDNTILYTDYIISEVVNKLKQQQTKFDTAMFYVSDHGESLGEKGMYLHGAPYGIAPIEQTSIP
MLAWVSEDFSQDNHLNMACLAKEAEKGGFSHDNLFDSLLGLMNVQTQVYQSKLDIFARCRG
>Mature_541_residues
MLTRFKTLTVNRFTFITALFYVCVFNIPLFEVIKKGIEKQPDVDPIFIASMPLFLTFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAALKYNVVFDYGMIENTFQTNSAEALMYVNFASILNMLLTGILPAYLIYKANIQYKPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDEIKRYIVPTYFIGAAAKYINVHYLQTPIEYQQLGLDAKNVTTNANGKPNLLVLVVGE
TARSMNYQYYGYDKPTNAYTEGQGLVAFKDTHSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAIDVLNHGGIKVQWFD
NDSGCKGVCDQVENITIDLNSDPELCSGQYCFDQVLLNKLDEALANAEQKDTVIALHVIGSHGPTYFLRYPPEHRKFTPD
CPRSDIQNCSAEELMNTYDNTILYTDYIISEVVNKLKQQQTKFDTAMFYVSDHGESLGEKGMYLHGAPYGIAPIEQTSIP
MLAWVSEDFSQDNHLNMACLAKEAEKGGFSHDNLFDSLLGLMNVQTQVYQSKLDIFARCRG

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=527, Percent_Identity=38.8994307400379, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI87082286, Length=472, Percent_Identity=25.8474576271186, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87082223, Length=444, Percent_Identity=23.6486486486486, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61216; Mature: 61216

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRFKTLTVNRFTFITALFYVCVFNIPLFEVIKKGIEKQPDVDPIFIASMPLFLTFALS
CCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHH
FVFSLFTVKYLVKPFFIILTLLSSSVFFAALKYNVVFDYGMIENTFQTNSAEALMYVNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEHH
SILNMLLTGILPAYLIYKANIQYKPFFKELLHKVLFMLAMLAGLGIIAFFYFQDYAAFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDEIKRYIVPTYFIGAAAKYINVHYLQTPIEYQQLGLDAKNVTTNANGKPNLLVLVVGE
CCHHHHHHCCHHHHHHHHHHHEEEEEEECCHHHHHHCCCHHCCCCCCCCCCCEEEEEECC
TARSMNYQYYGYDKPTNAYTEGQGLVAFKDTHSCGTATAVSLPCMFSRMGREDYDSRRAY
CHHHCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
AQDTAIDVLNHGGIKVQWFDNDSGCKGVCDQVENITIDLNSDPELCSGQYCFDQVLLNKL
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
DEALANAEQKDTVIALHVIGSHGPTYFLRYPPEHRKFTPDCPRSDIQNCSAEELMNTYDN
HHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHCC
TILYTDYIISEVVNKLKQQQTKFDTAMFYVSDHGESLGEKGMYLHGAPYGIAPIEQTSIP
EEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCC
MLAWVSEDFSQDNHLNMACLAKEAEKGGFSHDNLFDSLLGLMNVQTQVYQSKLDIFARCR
EEEEHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MLTRFKTLTVNRFTFITALFYVCVFNIPLFEVIKKGIEKQPDVDPIFIASMPLFLTFALS
CCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHH
FVFSLFTVKYLVKPFFIILTLLSSSVFFAALKYNVVFDYGMIENTFQTNSAEALMYVNFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEHH
SILNMLLTGILPAYLIYKANIQYKPFFKELLHKVLFMLAMLAGLGIIAFFYFQDYAAFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDEIKRYIVPTYFIGAAAKYINVHYLQTPIEYQQLGLDAKNVTTNANGKPNLLVLVVGE
CCHHHHHHCCHHHHHHHHHHHEEEEEEECCHHHHHHCCCHHCCCCCCCCCCCEEEEEECC
TARSMNYQYYGYDKPTNAYTEGQGLVAFKDTHSCGTATAVSLPCMFSRMGREDYDSRRAY
CHHHCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
AQDTAIDVLNHGGIKVQWFDNDSGCKGVCDQVENITIDLNSDPELCSGQYCFDQVLLNKL
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
DEALANAEQKDTVIALHVIGSHGPTYFLRYPPEHRKFTPDCPRSDIQNCSAEELMNTYDN
HHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHCC
TILYTDYIISEVVNKLKQQQTKFDTAMFYVSDHGESLGEKGMYLHGAPYGIAPIEQTSIP
EEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCC
MLAWVSEDFSQDNHLNMACLAKEAEKGGFSHDNLFDSLLGLMNVQTQVYQSKLDIFARCR
EEEEHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]