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The map label for this gene is pleD [H]

Identifier: 117920655

GI number: 117920655

Start: 2657947

End: 2659578

Strand: Direct

Name: pleD [H]

Synonym: Shewana3_2212

Alternate gene names: 117920655

Gene position: 2657947-2659578 (Clockwise)

Preceding gene: 117920652

Following gene: 117920666

Centisome position: 53.46

GC content: 47.06

Gene sequence:

>1632_bases
ATGAGTCTTGAAGAATCCTTAGAACAATTAAAACGTCAGTACTTGCATGCGCTACCAGATAAAAAGAAGCAAATCATTAC
CTTATGGATATCACTACGGAAAAACTGGCAAAGCCATATGCTGTCGGCCGTATACCGTGAGGTGCATAATTTAAAGGGCT
CCTGTGAAACTTTCGGCTTAACCGAAACCAAAGATATCGTCGATAGACTCGAATTACAGCTAAAAAACCTACTGGACGCT
CCTGCGCCAGAACTGCCCGTCATCAAGGGGTTAGACACCCTATTCCATCAACTGCTGCAAAATAATCTACGTAGTGAACC
CGCAGCACCCGTGGTGGAAGCGCAAATGCAGCAAAGCCCCTATAAGCCCACCAAAGCACGGCATGAGTATCGAATTGCGA
TTGTGGAGGATGACAGTAATGTCGGGGCGATGATCACTAAACAGCTGCATGAGTTTGGTTTTAATGTGCAGCATTTTTTA
AATTTCACCGATTTTCTGGGGATCCAAAATACCTCGCCCTTCGATTTAGTCTTACTCGATCTTATCTTGCCCGATTATAC
CGAGGCCGCTTTATTTACGGCGGCGACTGAATTTGAAAAAAACAACACCCGCGTGTTTGTTCTCTCTTCCCGCGGCGACT
TCGAGATGCGACTGCTGGCGATCCGCGCCAATGTCAGCGAGTATTTTGTCAAACCCGCCGAAACCACGCTACTGGTACGC
AAGATCCACCAATGGCTTAAAATGTCAGAAAAACAGCCGCTCAAAATTCTGCTAGTGGACGATCAGCAATCCATGGTCGA
TTATTTTTCCAGCCTACTTCGCAGTCATGGCCTAATGGTAAAAGGCATGACCAAACCCGAACAAGTATTGCCGACCCTTG
AGCAATTTGAGCCAGACTTGTTTATTTTTGATTTGTATATGCCCGATGTGAATGGTTTAGAGCTGGCAAAAATGATCCGC
CAATTAGACAAGTACAGCTCGAGTCCGATACTCGTGCTCAGCTCCGACGACACCATGCAGAACAAGGTCAGCATCATTCA
GGCGGGATCCGACGATTTAATCTCAAAGCAAACCGCGCCGAGTCTGTTTGTGACCCAAGTGATTTCCCGCGCCCAGCGTG
GCCATGATATTCGCAGCTCCGCCAGCCGTGATAGCTTAACCGGGCTGCTCAATCACACTCAGATCCTGGTTGCTGCACGC
CGCTGTTATAACCTCGCCAAACGTATCAACTCTTCAGTGTGCATTGCCATGCTGGATTTAGATCACTTTAAACAAGTAAA
TGATACCTATGGACATTCTGGCGGCGACAAAGTCCTTTTAGCCTTTGCCCACCTGTTGCAGCAATCTCTACGTCCTGTGG
ATTTTATGGGGCGTTACGGTGGCGAGGAATTTATGTTGGTCTTGCCCGATCTACCCGCGCCCTTAGCCATTGCCAAGCTT
AATGCCATTCGCGAGAGCTTTAGTCATATCGTATTTGTTGAAGAAGGCGCTGAATTTAAAGTCACCCTAAGCGGTGGCTT
GGCCTTCTCGACCGAATGTAATGAGTTTCAAGATTGTTTATTGCTGGCGGATAAAAATCTGTATGAGGCGAAGCGAACTG
GACGCAACCGTTTAATCACCACTTTAAAATAA

Upstream 100 bases:

>100_bases
CACAACTCAATGAATTGGGTGAATTAAACCTTATGGGGTATTGCATACGCGCCAGCTGTGCACCAATTTATTCTGTAAAT
TCTTATCATGGTGATGGTTT

Downstream 100 bases:

>100_bases
GCCTCAACGCATCAACGAGTTAATCTAAGACAGGACTTTAAAAAGTGACTTATGGCTATCAGTTGCATAAATGACGAAAC
TGATGCATACAAATAAGACT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSLEESLEQLKRQYLHALPDKKKQIITLWISLRKNWQSHMLSAVYREVHNLKGSCETFGLTETKDIVDRLELQLKNLLDA
PAPELPVIKGLDTLFHQLLQNNLRSEPAAPVVEAQMQQSPYKPTKARHEYRIAIVEDDSNVGAMITKQLHEFGFNVQHFL
NFTDFLGIQNTSPFDLVLLDLILPDYTEAALFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVR
KIHQWLKMSEKQPLKILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDLFIFDLYMPDVNGLELAKMIR
QLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVTQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAAR
RCYNLAKRINSSVCIAMLDLDHFKQVNDTYGHSGGDKVLLAFAHLLQQSLRPVDFMGRYGGEEFMLVLPDLPAPLAIAKL
NAIRESFSHIVFVEEGAEFKVTLSGGLAFSTECNEFQDCLLLADKNLYEAKRTGRNRLITTLK

Sequences:

>Translated_543_residues
MSLEESLEQLKRQYLHALPDKKKQIITLWISLRKNWQSHMLSAVYREVHNLKGSCETFGLTETKDIVDRLELQLKNLLDA
PAPELPVIKGLDTLFHQLLQNNLRSEPAAPVVEAQMQQSPYKPTKARHEYRIAIVEDDSNVGAMITKQLHEFGFNVQHFL
NFTDFLGIQNTSPFDLVLLDLILPDYTEAALFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVR
KIHQWLKMSEKQPLKILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDLFIFDLYMPDVNGLELAKMIR
QLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVTQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAAR
RCYNLAKRINSSVCIAMLDLDHFKQVNDTYGHSGGDKVLLAFAHLLQQSLRPVDFMGRYGGEEFMLVLPDLPAPLAIAKL
NAIRESFSHIVFVEEGAEFKVTLSGGLAFSTECNEFQDCLLLADKNLYEAKRTGRNRLITTLK
>Mature_542_residues
SLEESLEQLKRQYLHALPDKKKQIITLWISLRKNWQSHMLSAVYREVHNLKGSCETFGLTETKDIVDRLELQLKNLLDAP
APELPVIKGLDTLFHQLLQNNLRSEPAAPVVEAQMQQSPYKPTKARHEYRIAIVEDDSNVGAMITKQLHEFGFNVQHFLN
FTDFLGIQNTSPFDLVLLDLILPDYTEAALFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVRK
IHQWLKMSEKQPLKILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDLFIFDLYMPDVNGLELAKMIRQ
LDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVTQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARR
CYNLAKRINSSVCIAMLDLDHFKQVNDTYGHSGGDKVLLAFAHLLQQSLRPVDFMGRYGGEEFMLVLPDLPAPLAIAKLN
AIRESFSHIVFVEEGAEFKVTLSGGLAFSTECNEFQDCLLLADKNLYEAKRTGRNRLITTLK

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=241, Percent_Identity=27.3858921161826, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787262, Length=195, Percent_Identity=33.8461538461538, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=34.7560975609756, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=37.3493975903614, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=30.8641975308642, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=128, Percent_Identity=32.8125, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=32.7380952380952, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=31.9018404907975, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=31.4102564102564, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=128, Percent_Identity=34.375, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=84, Percent_Identity=39.2857142857143, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=31.7460317460317, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61533; Mature: 61402

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEESLEQLKRQYLHALPDKKKQIITLWISLRKNWQSHMLSAVYREVHNLKGSCETFGL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TETKDIVDRLELQLKNLLDAPAPELPVIKGLDTLFHQLLQNNLRSEPAAPVVEAQMQQSP
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
YKPTKARHEYRIAIVEDDSNVGAMITKQLHEFGFNVQHFLNFTDFLGIQNTSPFDLVLLD
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCHHHHCCCCCCCCHHHHHHH
LILPDYTEAALFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVR
HHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCHHHHHCCCCHHHHHHH
KIHQWLKMSEKQPLKILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDL
HHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCHHHCCCCE
FIFDLYMPDVNGLELAKMIRQLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCEEEEECCCCHHHHHHCCC
SLFVTQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARRCYNLAKRINSSVCIAMLDL
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECH
DHFKQVNDTYGHSGGDKVLLAFAHLLQQSLRPVDFMGRYGGEEFMLVLPDLPAPLAIAKL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCHHHHHH
NAIRESFSHIVFVEEGAEFKVTLSGGLAFSTECNEFQDCLLLADKNLYEAKRTGRNRLIT
HHHHHHHCEEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHHCCCHHHHHHCCCCCEEE
TLK
ECC
>Mature Secondary Structure 
SLEESLEQLKRQYLHALPDKKKQIITLWISLRKNWQSHMLSAVYREVHNLKGSCETFGL
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TETKDIVDRLELQLKNLLDAPAPELPVIKGLDTLFHQLLQNNLRSEPAAPVVEAQMQQSP
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
YKPTKARHEYRIAIVEDDSNVGAMITKQLHEFGFNVQHFLNFTDFLGIQNTSPFDLVLLD
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHCHHHHCCCCCCCCHHHHHHH
LILPDYTEAALFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVR
HHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCHHHHHCCCCHHHHHHH
KIHQWLKMSEKQPLKILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDL
HHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCHHHCCCCE
FIFDLYMPDVNGLELAKMIRQLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCEEEEECCCCHHHHHHCCC
SLFVTQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARRCYNLAKRINSSVCIAMLDL
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECH
DHFKQVNDTYGHSGGDKVLLAFAHLLQQSLRPVDFMGRYGGEEFMLVLPDLPAPLAIAKL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCHHHHHH
NAIRESFSHIVFVEEGAEFKVTLSGGLAFSTECNEFQDCLLLADKNLYEAKRTGRNRLIT
HHHHHHHCEEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHHCCCHHHHHHCCCCCEEE
TLK
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA