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The map label for this gene is ttcA1 [H]

Identifier: 117920561

GI number: 117920561

Start: 2540707

End: 2541675

Strand: Direct

Name: ttcA1 [H]

Synonym: Shewana3_2117

Alternate gene names: 117920561

Gene position: 2540707-2541675 (Clockwise)

Preceding gene: 117920560

Following gene: 117920564

Centisome position: 51.1

GC content: 47.78

Gene sequence:

>969_bases
ATGTTGGAAGCTATGTCTGAAGTTACGGCGAGTCAAATGCCTGAAGAATTATCAAAAAAACAAATTACCCGTCTGAATAA
ATTACAGAAGCGTCTGCGCCGTGAAGTGGGTAGTGCCATCGCCGATTACAACATGATTGAAGATGGCGACCGAGTCATGT
GCTGCTTAAGTGGCGGTAAAGACAGTTACGCCATGCTGGATATTCTGCTTAATCTACAGCAGCGCGCGCCCGTACAGTTC
GAAATTATTGCCGTTAATTTAGATCAAAAGCAGCCGGGTTTCCCTGAACACGTACTGCCAGCCTATTTAGACAGCCTTAA
TATTCCCTATCACATCCTCGAGAAAGACACTTACTCGATTGTGAAAGAAAAGATCCCTGAAGGCAAAACCACTTGCTCGC
TGTGCTCTCGCCTGCGTCGCGGTACTCTCTATGGCTTTGCCCAGCGCATTGGCGCAACCAAAATCGCACTAGGCCACCAC
AGAGACGATATTATTGAGACCATGTTCCTCAACATGTTCTACGGCGGCAAGATGAAAGCCATGCCACCTAAGTTGCTCTC
GGACGACGGCGCTAACGTGGTTATTCGTCCACTCGCCTATTGCCGCGAAAAGGATTTAGAAGAATATGCGGAATTGAAAA
AATTCCCCATCATTCCCTGTAATCTTTGTGGCTCGCAGGAAAACCTCAAGCGTCAAGCAGTGAAAGACATGCTCAATCAG
TGGGATAGACTCTACCCAGGCCGTATTGAAACTATTTTCACCGCCATGCAAAACACTGCCCCTTCACAGGGTGTTGACCG
TGAGCAGTTTGACTTTGTGTCGTTGAAACGCGATCCCAACGCGCCAATGAAGGGTGATGTGGCCGAAGCCGATTTACCCG
CATTTGACTTTTTGGATATCGCCAACAGCGGCCATATCGATTTAGATGCGGCAAAACGCATCAATATCGTCAACGTTTAC
GAAGCGTAA

Upstream 100 bases:

>100_bases
AATCACCGCTTGAAGAGGCATAACGCACGCCGCTCTTTACCCCGCCGTCGATAGCTGGAATAATACGCGCCCTGAAACTG
AGTTACTGGGCGCTTTTTTT

Downstream 100 bases:

>100_bases
ACGCGATACGCATAAAAAAAGCCCACGAGAGTGGGCTTTTTTATAGCTTAAATCTATCCTTACTTAACCCAAGGGCTCAA
CACGGTCGGCGCTTGGCTAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MLEAMSEVTASQMPEELSKKQITRLNKLQKRLRREVGSAIADYNMIEDGDRVMCCLSGGKDSYAMLDILLNLQQRAPVQF
EIIAVNLDQKQPGFPEHVLPAYLDSLNIPYHILEKDTYSIVKEKIPEGKTTCSLCSRLRRGTLYGFAQRIGATKIALGHH
RDDIIETMFLNMFYGGKMKAMPPKLLSDDGANVVIRPLAYCREKDLEEYAELKKFPIIPCNLCGSQENLKRQAVKDMLNQ
WDRLYPGRIETIFTAMQNTAPSQGVDREQFDFVSLKRDPNAPMKGDVAEADLPAFDFLDIANSGHIDLDAAKRINIVNVY
EA

Sequences:

>Translated_322_residues
MLEAMSEVTASQMPEELSKKQITRLNKLQKRLRREVGSAIADYNMIEDGDRVMCCLSGGKDSYAMLDILLNLQQRAPVQF
EIIAVNLDQKQPGFPEHVLPAYLDSLNIPYHILEKDTYSIVKEKIPEGKTTCSLCSRLRRGTLYGFAQRIGATKIALGHH
RDDIIETMFLNMFYGGKMKAMPPKLLSDDGANVVIRPLAYCREKDLEEYAELKKFPIIPCNLCGSQENLKRQAVKDMLNQ
WDRLYPGRIETIFTAMQNTAPSQGVDREQFDFVSLKRDPNAPMKGDVAEADLPAFDFLDIANSGHIDLDAAKRINIVNVY
EA
>Mature_322_residues
MLEAMSEVTASQMPEELSKKQITRLNKLQKRLRREVGSAIADYNMIEDGDRVMCCLSGGKDSYAMLDILLNLQQRAPVQF
EIIAVNLDQKQPGFPEHVLPAYLDSLNIPYHILEKDTYSIVKEKIPEGKTTCSLCSRLRRGTLYGFAQRIGATKIALGHH
RDDIIETMFLNMFYGGKMKAMPPKLLSDDGANVVIRPLAYCREKDLEEYAELKKFPIIPCNLCGSQENLKRQAVKDMLNQ
WDRLYPGRIETIFTAMQNTAPSQGVDREQFDFVSLKRDPNAPMKGDVAEADLPAFDFLDIANSGHIDLDAAKRINIVNVY
EA

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=277, Percent_Identity=67.870036101083, Blast_Score=409, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI193211090, Length=263, Percent_Identity=34.6007604562738, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17540090, Length=185, Percent_Identity=28.6486486486486, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36394; Mature: 36394

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAMSEVTASQMPEELSKKQITRLNKLQKRLRREVGSAIADYNMIEDGDRVMCCLSGGK
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
DSYAMLDILLNLQQRAPVQFEIIAVNLDQKQPGFPEHVLPAYLDSLNIPYHILEKDTYSI
CHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
VKEKIPEGKTTCSLCSRLRRGTLYGFAQRIGATKIALGHHRDDIIETMFLNMFYGGKMKA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHCCCCCCC
MPPKLLSDDGANVVIRPLAYCREKDLEEYAELKKFPIIPCNLCGSQENLKRQAVKDMLNQ
CCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
WDRLYPGRIETIFTAMQNTAPSQGVDREQFDFVSLKRDPNAPMKGDVAEADLPAFDFLDI
HHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHC
ANSGHIDLDAAKRINIVNVYEA
CCCCCEEECHHHCEEEEEEECC
>Mature Secondary Structure
MLEAMSEVTASQMPEELSKKQITRLNKLQKRLRREVGSAIADYNMIEDGDRVMCCLSGGK
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
DSYAMLDILLNLQQRAPVQFEIIAVNLDQKQPGFPEHVLPAYLDSLNIPYHILEKDTYSI
CHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHH
VKEKIPEGKTTCSLCSRLRRGTLYGFAQRIGATKIALGHHRDDIIETMFLNMFYGGKMKA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHCCCCCCC
MPPKLLSDDGANVVIRPLAYCREKDLEEYAELKKFPIIPCNLCGSQENLKRQAVKDMLNQ
CCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
WDRLYPGRIETIFTAMQNTAPSQGVDREQFDFVSLKRDPNAPMKGDVAEADLPAFDFLDI
HHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHC
ANSGHIDLDAAKRINIVNVYEA
CCCCCEEECHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA