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The map label for this gene is fdnI [H]

Identifier: 117920535

GI number: 117920535

Start: 2510700

End: 2511323

Strand: Direct

Name: fdnI [H]

Synonym: Shewana3_2091

Alternate gene names: 117920535

Gene position: 2510700-2511323 (Clockwise)

Preceding gene: 117920534

Following gene: 117920536

Centisome position: 50.49

GC content: 49.84

Gene sequence:

>624_bases
ATGAATCATAAAGAAATGATTGTCCGTCATAAGCTCGTTGATCGCCTGTGCCACTGGACCATAGTCTTTGTTGGCCTAGT
GACCTTTCTAACGGGGTTCTCGTTTTTTTATCCCTCGTTCCAGTGGCTAAGTGGCATTGTTGGCACACCACAACTGGCAA
AAGTGATGCATCCATTCTTCGGGATCGCCATGTGTCTGCTGCTGGCGGTGATGTTGATCCGCTACTACCAGCACAATAAG
TGGAATAAATACGATTTGCCCTGGATGAAAGCCGTGGTGTGGGTGTTACTGCAAAAAGAAGAGAACATTCCGCCCGTGGG
CCATTACAACCCAGGTCAGAAAATGTTATTCCGCGCCTTTGTGGTTTTTGTAATGGCGTTTCTGATCAGCGGCATCATGA
TGTGGCAGCCTTATTTTGCGCCCATGTTCTCGGCAGAAGCCGTCAATTGGGCCACCTTAGTCCACTCGGCCTGCGGCGTG
TTAATGCTGCTCTCCCTCGTGGTACATGTGTGGATGGCCTGCTGGATTGAAGGCTCTATCACAGGGATGCTCTACGGCAA
AGTCTCTAAGGCTTGGGCCCGTAAGCACCATCCAGCGATGTTAGAAGAACACGAGGAAAAATAG

Upstream 100 bases:

>100_bases
CGGCATTCGCCCTGATGCACCGCATTGCAGTAGGGCGCAACATCGTCGAGGAAGATCAGCCTGGTTACATTGATGCCGGT
GTCAAAGAGGAGGAAAAGCT

Downstream 100 bases:

>100_bases
CAATGAAGATGGCCAGCGATATCCCGTTAAGTTTTGTTGATAAATCCCCCTTAGCATTGAAACCGCTAAAAGCGGCAGAT
CCTGAGAAAATTTATCAACA

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MNHKEMIVRHKLVDRLCHWTIVFVGLVTFLTGFSFFYPSFQWLSGIVGTPQLAKVMHPFFGIAMCLLLAVMLIRYYQHNK
WNKYDLPWMKAVVWVLLQKEENIPPVGHYNPGQKMLFRAFVVFVMAFLISGIMMWQPYFAPMFSAEAVNWATLVHSACGV
LMLLSLVVHVWMACWIEGSITGMLYGKVSKAWARKHHPAMLEEHEEK

Sequences:

>Translated_207_residues
MNHKEMIVRHKLVDRLCHWTIVFVGLVTFLTGFSFFYPSFQWLSGIVGTPQLAKVMHPFFGIAMCLLLAVMLIRYYQHNK
WNKYDLPWMKAVVWVLLQKEENIPPVGHYNPGQKMLFRAFVVFVMAFLISGIMMWQPYFAPMFSAEAVNWATLVHSACGV
LMLLSLVVHVWMACWIEGSITGMLYGKVSKAWARKHHPAMLEEHEEK
>Mature_207_residues
MNHKEMIVRHKLVDRLCHWTIVFVGLVTFLTGFSFFYPSFQWLSGIVGTPQLAKVMHPFFGIAMCLLLAVMLIRYYQHNK
WNKYDLPWMKAVVWVLLQKEENIPPVGHYNPGQKMLFRAFVVFVMAFLISGIMMWQPYFAPMFSAEAVNWATLVHSACGV
LMLLSLVVHVWMACWIEGSITGMLYGKVSKAWARKHHPAMLEEHEEK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=206, Percent_Identity=43.2038834951456, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1790325, Length=203, Percent_Identity=38.9162561576355, Blast_Score=176, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24051; Mature: 24051

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
7.2 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
7.2 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKEMIVRHKLVDRLCHWTIVFVGLVTFLTGFSFFYPSFQWLSGIVGTPQLAKVMHPFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
GIAMCLLLAVMLIRYYQHNKWNKYDLPWMKAVVWVLLQKEENIPPVGHYNPGQKMLFRAF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VVFVMAFLISGIMMWQPYFAPMFSAEAVNWATLVHSACGVLMLLSLVVHVWMACWIEGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TGMLYGKVSKAWARKHHPAMLEEHEEK
HHHHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MNHKEMIVRHKLVDRLCHWTIVFVGLVTFLTGFSFFYPSFQWLSGIVGTPQLAKVMHPFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
GIAMCLLLAVMLIRYYQHNKWNKYDLPWMKAVVWVLLQKEENIPPVGHYNPGQKMLFRAF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VVFVMAFLISGIMMWQPYFAPMFSAEAVNWATLVHSACGVLMLLSLVVHVWMACWIEGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TGMLYGKVSKAWARKHHPAMLEEHEEK
HHHHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]