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The map label for this gene is fdnH [H]

Identifier: 117920534

GI number: 117920534

Start: 2509801

End: 2510703

Strand: Direct

Name: fdnH [H]

Synonym: Shewana3_2090

Alternate gene names: 117920534

Gene position: 2509801-2510703 (Clockwise)

Preceding gene: 117920533

Following gene: 117920535

Centisome position: 50.48

GC content: 53.05

Gene sequence:

>903_bases
ATGGCTTCTCAAGATATTATCCGCATCTCAGGCACCACGGATATCACCCCTGCGTCCCAGGCGCGCATTGGTGCGGTCCA
GCAGGTGACTAAGCTGTTTGATGCGACTAAATGTACCGGCTGTAAAGCGTGTCAGGTTGCCTGCTCCGAATGGAATGACC
TTCGTGAAGATGTCGGCACTTTCCAAGGCAGTTACCAGAACCCGATGATACTGTCTCCAGAAAGCTGGAACATAATGCAG
TATCACGAAGTGATGGATAACAATAAGTTGCGTTGGCAATTTACCCACACTGCCTGTATGCACTGCGCCGATCCAGCCTG
CTTAAAAGCCTGCTCAACCAGCGGCGCGATTGTGCAACATGCCAATGGCACAGTGGATTTTGACTCGAACAAGTGTATTG
GCTGCGGCTATTGTGCCAGTGCCTGTCCTTTCAATGTGCCTAAGATCAGTGCCAAAGATAACAAAGCCTATAAGTGCACC
CTGTGCTCCGATAGGCTCGCCGTTGGCCTCGAGCCTTCCTGCGTTAAAACCTGCACCGTGGGAGCGCTGCGTTTTGGCAC
CCGCGAAGATATGTTGTTCTATGCCGAAAAGCGCGTCGAGCAGTTAAAGGAACGCGGTTTTACCAAGGCGGGACTCTATA
ACCCCGAAGGGGTTGGTGGTACGCATATGATGATGATCCTCCACGACATCACGCAACCCGAAACCTATGGTATGCCCAAG
GATCCGCAAATCCCCGCCGCCACTGAATGGCGTCAAGATTGGCTTAAACCTTTGGGCACCGCGGGCTTGCTCGCAACGGC
GGCATTCGCCCTGATGCACCGCATTGCAGTAGGGCGCAACATCGTCGAGGAAGATCAGCCTGGTTACATTGATGCCGGTG
TCAAAGAGGAGGAAAAGCTATGA

Upstream 100 bases:

>100_bases
TCGTCAACGAGCTGACTTCATCTGTGGGCGATGCCAATACCCAAACCCCTGAATATAAAGCATTCCTTGTGAATATTGCC
AAAGCGGAGGGCTTCTAATT

Downstream 100 bases:

>100_bases
ATCATAAAGAAATGATTGTCCGTCATAAGCTCGTTGATCGCCTGTGCCACTGGACCATAGTCTTTGTTGGCCTAGTGACC
TTTCTAACGGGGTTCTCGTT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MASQDIIRISGTTDITPASQARIGAVQQVTKLFDATKCTGCKACQVACSEWNDLREDVGTFQGSYQNPMILSPESWNIMQ
YHEVMDNNKLRWQFTHTACMHCADPACLKACSTSGAIVQHANGTVDFDSNKCIGCGYCASACPFNVPKISAKDNKAYKCT
LCSDRLAVGLEPSCVKTCTVGALRFGTREDMLFYAEKRVEQLKERGFTKAGLYNPEGVGGTHMMMILHDITQPETYGMPK
DPQIPAATEWRQDWLKPLGTAGLLATAAFALMHRIAVGRNIVEEDQPGYIDAGVKEEEKL

Sequences:

>Translated_300_residues
MASQDIIRISGTTDITPASQARIGAVQQVTKLFDATKCTGCKACQVACSEWNDLREDVGTFQGSYQNPMILSPESWNIMQ
YHEVMDNNKLRWQFTHTACMHCADPACLKACSTSGAIVQHANGTVDFDSNKCIGCGYCASACPFNVPKISAKDNKAYKCT
LCSDRLAVGLEPSCVKTCTVGALRFGTREDMLFYAEKRVEQLKERGFTKAGLYNPEGVGGTHMMMILHDITQPETYGMPK
DPQIPAATEWRQDWLKPLGTAGLLATAAFALMHRIAVGRNIVEEDQPGYIDAGVKEEEKL
>Mature_299_residues
ASQDIIRISGTTDITPASQARIGAVQQVTKLFDATKCTGCKACQVACSEWNDLREDVGTFQGSYQNPMILSPESWNIMQY
HEVMDNNKLRWQFTHTACMHCADPACLKACSTSGAIVQHANGTVDFDSNKCIGCGYCASACPFNVPKISAKDNKAYKCTL
CSDRLAVGLEPSCVKTCTVGALRFGTREDMLFYAEKRVEQLKERGFTKAGLYNPEGVGGTHMMMILHDITQPETYGMPKD
PQIPAATEWRQDWLKPLGTAGLLATAAFALMHRIAVGRNIVEEDQPGYIDAGVKEEEKL

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=288, Percent_Identity=54.5138888888889, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1790326, Length=285, Percent_Identity=52.6315789473684, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI1789370, Length=193, Percent_Identity=34.1968911917098, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787872, Length=164, Percent_Identity=35.9756097560976, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787122, Length=164, Percent_Identity=35.3658536585366, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI2367345, Length=183, Percent_Identity=30.6010928961749, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI226510944, Length=191, Percent_Identity=31.413612565445, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788811, Length=174, Percent_Identity=28.1609195402299, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=30.0613496932515, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=103, Percent_Identity=32.0388349514563, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32937; Mature: 32806

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQDIIRISGTTDITPASQARIGAVQQVTKLFDATKCTGCKACQVACSEWNDLREDVGT
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FQGSYQNPMILSPESWNIMQYHEVMDNNKLRWQFTHTACMHCADPACLKACSTSGAIVQH
HCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCEEEC
ANGTVDFDSNKCIGCGYCASACPFNVPKISAKDNKAYKCTLCSDRLAVGLEPSCVKTCTV
CCCEEEECCCCEEECCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
GALRFGTREDMLFYAEKRVEQLKERGFTKAGLYNPEGVGGTHMMMILHDITQPETYGMPK
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCC
DPQIPAATEWRQDWLKPLGTAGLLATAAFALMHRIAVGRNIVEEDQPGYIDAGVKEEEKL
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEECCCCHHCCC
>Mature Secondary Structure 
ASQDIIRISGTTDITPASQARIGAVQQVTKLFDATKCTGCKACQVACSEWNDLREDVGT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FQGSYQNPMILSPESWNIMQYHEVMDNNKLRWQFTHTACMHCADPACLKACSTSGAIVQH
HCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHCCCCCEEEC
ANGTVDFDSNKCIGCGYCASACPFNVPKISAKDNKAYKCTLCSDRLAVGLEPSCVKTCTV
CCCEEEECCCCEEECCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
GALRFGTREDMLFYAEKRVEQLKERGFTKAGLYNPEGVGGTHMMMILHDITQPETYGMPK
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCC
DPQIPAATEWRQDWLKPLGTAGLLATAAFALMHRIAVGRNIVEEDQPGYIDAGVKEEEKL
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]