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The map label for this gene is gph [C]

Identifier: 117920415

GI number: 117920415

Start: 2346063

End: 2346737

Strand: Reverse

Name: gph [C]

Synonym: Shewana3_1970

Alternate gene names: 117920415

Gene position: 2346737-2346063 (Counterclockwise)

Preceding gene: 117920416

Following gene: 117920414

Centisome position: 47.2

GC content: 54.81

Gene sequence:

>675_bases
ATGAGTTTAGCTGAGGTTAAAGGGGTCTTATTCGACCTTGACGGTACCCTTGCCGATACCGCGCCGGATCTGGTTCAGGC
CCTTAACCTCAGTTTGTCGGATGTCGGTATCGCAGCAAAACCTTTAGCAGATATGCGTAGCGCTGCCTCCCATGGCAGCT
TTGCCTTAGTCGATGCGGCGATTCCCGGCGCGGATGACGCCCTGCGCACTCAAGTACAGCAAGGACTCCTCGCCCACTAT
CAAAGGATCAATGGCGACCATTGCCAGTTGTTTGATGGTATCGCCCCCCTGCTCGATTGGCTCGAGTTGTGCCGCGTACC
ATTTGGGGTGATCACCAATAAACCCGCCCGCTTTACTCGCCCTCTATTGCATAAACTTAAGCTCAGCTCACGTATGCCGG
TGATGATCAGTGGCGATTCCACTCGTTATCCCAAGCCCCATACCGCACCAATGCTATTGGGCGCACAGCAATTGCAGTGC
CTACCACAGCAAATCCTCTATTTAGGGGATGCCGAGCGGGATTTACTCGCGGCCCAAGCGGCGGGCATGCTCGGCGTTAT
AGCGCTGTGGGGATATTTGGGTGAGGCCGATACGCCCGATGCATGGCCTGCCTATGCACAGTTTACCTCGCCATCGAGTT
TGCATTCGGCATTAAGCCAAGCATTAGCGAAATAG

Upstream 100 bases:

>100_bases
GATGCACTTGGGATCACCTATAACCCACTGACGGGGATCTTTAAGTACACGCCAAAGGTCGATGTTAATTATATGATTGC
GACGCAAAAGGTAGATTAAG

Downstream 100 bases:

>100_bases
CGGTAACGGACCGACTCGTGCTAATTGCTCAACTTAACCATTAATGGCGAAATTCTGCCCCATTTGCTGGTAATGCAATT
TAGGCTAAGGGTCAATCCTT

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSLAEVKGVLFDLDGTLADTAPDLVQALNLSLSDVGIAAKPLADMRSAASHGSFALVDAAIPGADDALRTQVQQGLLAHY
QRINGDHCQLFDGIAPLLDWLELCRVPFGVITNKPARFTRPLLHKLKLSSRMPVMISGDSTRYPKPHTAPMLLGAQQLQC
LPQQILYLGDAERDLLAAQAAGMLGVIALWGYLGEADTPDAWPAYAQFTSPSSLHSALSQALAK

Sequences:

>Translated_224_residues
MSLAEVKGVLFDLDGTLADTAPDLVQALNLSLSDVGIAAKPLADMRSAASHGSFALVDAAIPGADDALRTQVQQGLLAHY
QRINGDHCQLFDGIAPLLDWLELCRVPFGVITNKPARFTRPLLHKLKLSSRMPVMISGDSTRYPKPHTAPMLLGAQQLQC
LPQQILYLGDAERDLLAAQAAGMLGVIALWGYLGEADTPDAWPAYAQFTSPSSLHSALSQALAK
>Mature_223_residues
SLAEVKGVLFDLDGTLADTAPDLVQALNLSLSDVGIAAKPLADMRSAASHGSFALVDAAIPGADDALRTQVQQGLLAHYQ
RINGDHCQLFDGIAPLLDWLELCRVPFGVITNKPARFTRPLLHKLKLSSRMPVMISGDSTRYPKPHTAPMLLGAQQLQCL
PQQILYLGDAERDLLAAQAAGMLGVIALWGYLGEADTPDAWPAYAQFTSPSSLHSALSQALAK

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=217, Percent_Identity=31.7972350230415, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23938; Mature: 23807

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAEVKGVLFDLDGTLADTAPDLVQALNLSLSDVGIAAKPLADMRSAASHGSFALVDAA
CCHHHHHHHHEECCCCHHCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEECC
IPGADDALRTQVQQGLLAHYQRINGDHCQLFDGIAPLLDWLELCRVPFGVITNKPARFTR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHH
PLLHKLKLSSRMPVMISGDSTRYPKPHTAPMLLGAQQLQCLPQQILYLGDAERDLLAAQA
HHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGMLGVIALWGYLGEADTPDAWPAYAQFTSPSSLHSALSQALAK
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLAEVKGVLFDLDGTLADTAPDLVQALNLSLSDVGIAAKPLADMRSAASHGSFALVDAA
CHHHHHHHHEECCCCHHCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEECC
IPGADDALRTQVQQGLLAHYQRINGDHCQLFDGIAPLLDWLELCRVPFGVITNKPARFTR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHH
PLLHKLKLSSRMPVMISGDSTRYPKPHTAPMLLGAQQLQCLPQQILYLGDAERDLLAAQA
HHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AGMLGVIALWGYLGEADTPDAWPAYAQFTSPSSLHSALSQALAK
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA