Click here to switch to the map view.

The map label for this gene is nrdA [H]

Identifier: 117920414

GI number: 117920414

Start: 2343289

End: 2345577

Strand: Reverse

Name: nrdA [H]

Synonym: Shewana3_1969

Alternate gene names: 117920414

Gene position: 2345577-2343289 (Counterclockwise)

Preceding gene: 117920415

Following gene: 117920413

Centisome position: 47.17

GC content: 47.18

Gene sequence:

>2289_bases
ATGAATAGCAATATGACAGTCACAAAACGCTGTGGTGCACGTGAGACAATCGATCTCGATAAGATACACCGCGTAATTAC
TTGGGCAGCCAAGGGATTAAAAAACGTTTCTGTTTCTGAAGTTGAACTTCGCACGCATTTACAGTTTTTCGACGGGATCC
CAACCGAAGCTATCCACGAAACGATCATCAAATCTGCGGCGGATTTAATTTCCCCAGAATCACCGGATTATCAGTTTCTG
GCTGCGCGCTTAGCCGTATTCCATTTACGTAAGAAGGCCTTTGGTCAGTTCGAGCCGCCAAAGTTATACGACCATGTGAC
TAAACTGGTCGAGCTTGGCAAGTATGATATGCATATTCTGCAGGATTACACCCGCGAAGAACTCGATATTATGGACAGCT
ATATCGACCACTGGCGCGATATGAACTTCTCCTACGCGGCAGTAAAACAGCTCGAAGGTAAGTATCTGGTACAAAACCGT
GTGACCCACGAGATCTACGAGAGCGCCCAGTTCCTCTATATTCTAGTGGCAGCCTGTTTGTTTGCCCGTTATCCAAAAGA
AACGCGCCTGCAATATGTTAAAGATTTTTACGACGCGGTATCGACCTTTAAGATTTCGCTGCCAACGCCAATCATGGCAG
GTGTACGCACGCCAACACGTCAATTCAGTTCATGCGTACTGATTGAATGTGGCGACAGCTTAGATTCTATCAACGCGACC
GCCTCTTCTATCGTGAAGTATGTGAGCCAACGCGCAGGTATCGGTATCAACGCGGGTCGTATCCGTGCATTAGGTAGCCC
AATCCGTGGCGGCGAAGCCTTCCACACCGGTTGCTTACCTTTCTACAAGTATTTCCAAACGGCGGTAAAATCTTGCTCGC
AAGGCGGCGTTCGTGGTGGTGCAGCGACCCTCTTCTACCCCATTTGGCACTTAGAAGTAGAATCGCTGCTGGTACTGAAA
AACAACCGCGGTGTTGAAGATAACCGTATTCGCCACTTAGACTACGGCGTGCAGCTCAACAAGCTGATGTACCAACGTCT
GATCAAAGGCGAAAACATTAGCCTGTTCAGCCCATCTGACGTACCAGGTCTTTACGATGCCTTCTTCGAAGATCAAGACG
AATTCGAACGTCTGTACCTGCAATATGAGCAAGACAGCAGCATTCGTAAGAAGACATTGAAAGCAGTTGAACTGTTCTCA
TTGATGATGCAAGAGCGTGCTTCAACGGGCCGTATCTACATCCAAAACGTCGACCACTGTAATACCCACAGTCCGTTCGA
CTCGAAAGTCGCTCCGATCAGACAATCTAACCTTTGTCTTGAAATCGCGCTGCCGACTAAGCCGTTGAACAACATTGACG
ATCCAAACGGTGAAATCGCGCTGTGTACACTGTCAGCGTTAAACTTAGGTGCAATCAAAAACTTAGCCGAGCTTGAGCCA
TTAGCCGATTTAGCGGTACGTGCACTGGATAACCTACTGGATTACCAAGATTATCCAATCAAAGCGGCCGAAATTTCATC
GATGAACCGTCGTACCTTAGGTATTGGTGTTATCAACTTTGCGAACTACTTAGCGAAGAATGGCATGAAGTACTCCGATG
GTAGCGCGAACAACCTGACTCACAAGACCTTTGAAGCGATTCAGTTCTATCTGCTCAAGGCGTCAATGAATCTGGCGAAA
GAGAAAGGTGCGTGCCCATTATTTAATGAGACCACTTACGCTCAAGGTATTTTACCTATCGATACCTACAAACGTGATTT
AGATAAGATTTGCAGCGAGCCATTGCATTTAGACTGGGAAACACTGCGCCAAGACATCAAACAGCACGGTTTACGTAACT
CGACCCTGTCTGCATTGATGCCGTCTGAGACTTCATCACAGATCTCAAACGCGACCAACGGTATCGAACCGCCACGGGGT
TTGATCAGCGTTAAAGCGAGTAAAGATGGCCAGTTAAAGCAAGTGGTGCCTGATTTTGATGAGCTGAAATACAACTACGA
GCTGCTATGGCAAATGCCAAGCAACGAAGGTTACTTACAGCTCGTGGGTATCATGCAAAAATTCGTTGACCAAGCGATTT
CGGCCAACACTAACTATGACCCAACGCGTTTCGAAGGCCGTAAGGTTCCGATGCAGACTCTGCTGAAAGACTTACTCAAT
GCCTATAAGTTAGGTGTAAAAACGCTGTACTACCATAACACTCGCGATGGTGCATCGGATCAACACGATGACATTACCAA
CATAGAGAAAGAAGATGACGGCTGTGCTGGCGGCGCCTGCAAAATCTAA

Upstream 100 bases:

>100_bases
TAGTCAGCAGTATTAAGGGTTTTCAGGTTGATTGTTTTACAAGGAAACGTACGGTGACTTAGTTCACCTGAACAGATATC
AGAACCAAGGCCTCTCTTCA

Downstream 100 bases:

>100_bases
TCTTCAATGTTAACGGTTGCGGGATCCCTTTGGGATCCCGACTTATTGGAATACTCGTACCATGACCTACTCTACTTTTT
GCCAAACACCCAACGATGCC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MNSNMTVTKRCGARETIDLDKIHRVITWAAKGLKNVSVSEVELRTHLQFFDGIPTEAIHETIIKSAADLISPESPDYQFL
AARLAVFHLRKKAFGQFEPPKLYDHVTKLVELGKYDMHILQDYTREELDIMDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFARYPKETRLQYVKDFYDAVSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPIWHLEVESLLVLK
NNRGVEDNRIRHLDYGVQLNKLMYQRLIKGENISLFSPSDVPGLYDAFFEDQDEFERLYLQYEQDSSIRKKTLKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLNNIDDPNGEIALCTLSALNLGAIKNLAELEP
LADLAVRALDNLLDYQDYPIKAAEISSMNRRTLGIGVINFANYLAKNGMKYSDGSANNLTHKTFEAIQFYLLKASMNLAK
EKGACPLFNETTYAQGILPIDTYKRDLDKICSEPLHLDWETLRQDIKQHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFDELKYNYELLWQMPSNEGYLQLVGIMQKFVDQAISANTNYDPTRFEGRKVPMQTLLKDLLN
AYKLGVKTLYYHNTRDGASDQHDDITNIEKEDDGCAGGACKI

Sequences:

>Translated_762_residues
MNSNMTVTKRCGARETIDLDKIHRVITWAAKGLKNVSVSEVELRTHLQFFDGIPTEAIHETIIKSAADLISPESPDYQFL
AARLAVFHLRKKAFGQFEPPKLYDHVTKLVELGKYDMHILQDYTREELDIMDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFARYPKETRLQYVKDFYDAVSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPIWHLEVESLLVLK
NNRGVEDNRIRHLDYGVQLNKLMYQRLIKGENISLFSPSDVPGLYDAFFEDQDEFERLYLQYEQDSSIRKKTLKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLNNIDDPNGEIALCTLSALNLGAIKNLAELEP
LADLAVRALDNLLDYQDYPIKAAEISSMNRRTLGIGVINFANYLAKNGMKYSDGSANNLTHKTFEAIQFYLLKASMNLAK
EKGACPLFNETTYAQGILPIDTYKRDLDKICSEPLHLDWETLRQDIKQHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFDELKYNYELLWQMPSNEGYLQLVGIMQKFVDQAISANTNYDPTRFEGRKVPMQTLLKDLLN
AYKLGVKTLYYHNTRDGASDQHDDITNIEKEDDGCAGGACKI
>Mature_762_residues
MNSNMTVTKRCGARETIDLDKIHRVITWAAKGLKNVSVSEVELRTHLQFFDGIPTEAIHETIIKSAADLISPESPDYQFL
AARLAVFHLRKKAFGQFEPPKLYDHVTKLVELGKYDMHILQDYTREELDIMDSYIDHWRDMNFSYAAVKQLEGKYLVQNR
VTHEIYESAQFLYILVAACLFARYPKETRLQYVKDFYDAVSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGGAATLFYPIWHLEVESLLVLK
NNRGVEDNRIRHLDYGVQLNKLMYQRLIKGENISLFSPSDVPGLYDAFFEDQDEFERLYLQYEQDSSIRKKTLKAVELFS
LMMQERASTGRIYIQNVDHCNTHSPFDSKVAPIRQSNLCLEIALPTKPLNNIDDPNGEIALCTLSALNLGAIKNLAELEP
LADLAVRALDNLLDYQDYPIKAAEISSMNRRTLGIGVINFANYLAKNGMKYSDGSANNLTHKTFEAIQFYLLKASMNLAK
EKGACPLFNETTYAQGILPIDTYKRDLDKICSEPLHLDWETLRQDIKQHGLRNSTLSALMPSETSSQISNATNGIEPPRG
LISVKASKDGQLKQVVPDFDELKYNYELLWQMPSNEGYLQLVGIMQKFVDQAISANTNYDPTRFEGRKVPMQTLLKDLLN
AYKLGVKTLYYHNTRDGASDQHDDITNIEKEDDGCAGGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=789, Percent_Identity=27.6299112801014, Blast_Score=238, Evalue=1e-62,
Organism=Escherichia coli, GI2367133, Length=762, Percent_Identity=80.9711286089239, Blast_Score=1306, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=656, Percent_Identity=24.390243902439, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=811, Percent_Identity=26.387176325524, Blast_Score=233, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322123, Length=674, Percent_Identity=29.2284866468843, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6320914, Length=674, Percent_Identity=29.2284866468843, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17352481, Length=705, Percent_Identity=29.645390070922, Blast_Score=246, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85932; Mature: 85932

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNMTVTKRCGARETIDLDKIHRVITWAAKGLKNVSVSEVELRTHLQFFDGIPTEAIHE
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHH
TIIKSAADLISPESPDYQFLAARLAVFHLRKKAFGQFEPPKLYDHVTKLVELGKYDMHIL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHH
QDYTREELDIMDSYIDHWRDMNFSYAAVKQLEGKYLVQNRVTHEIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FARYPKETRLQYVKDFYDAVSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHHHHHEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHLEVESLLVLKNNRGVEDNRIRHLDYGVQLNKLMYQRLIKGENISLFSPSD
CHHEEHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFEDQDEFERLYLQYEQDSSIRKKTLKAVELFSLMMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPIRQSNLCLEIALPTKPLNNIDDPNGEIALCTLSALNLGAIKNLAELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIKAAEISSMNRRTLGIGVINFANYLAKNGMKYSDGSANNLT
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCH
HKTFEAIQFYLLKASMNLAKEKGACPLFNETTYAQGILPIDTYKRDLDKICSEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHH
TLRQDIKQHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFD
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
ELKYNYELLWQMPSNEGYLQLVGIMQKFVDQAISANTNYDPTRFEGRKVPMQTLLKDLLN
HHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGVKTLYYHNTRDGASDQHDDITNIEKEDDGCAGGACKI
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSNMTVTKRCGARETIDLDKIHRVITWAAKGLKNVSVSEVELRTHLQFFDGIPTEAIHE
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHH
TIIKSAADLISPESPDYQFLAARLAVFHLRKKAFGQFEPPKLYDHVTKLVELGKYDMHIL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHH
QDYTREELDIMDSYIDHWRDMNFSYAAVKQLEGKYLVQNRVTHEIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FARYPKETRLQYVKDFYDAVSTFKISLPTPIMAGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCHHHHHHHHHHHHHHHHEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCLPFYKYFQTAVKSCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHLEVESLLVLKNNRGVEDNRIRHLDYGVQLNKLMYQRLIKGENISLFSPSD
CHHEEHHHHHEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFEDQDEFERLYLQYEQDSSIRKKTLKAVELFSLMMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDSKVAPIRQSNLCLEIALPTKPLNNIDDPNGEIALCTLSALNLGAIKNLAELEP
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHCHHHHHHHHHHHH
LADLAVRALDNLLDYQDYPIKAAEISSMNRRTLGIGVINFANYLAKNGMKYSDGSANNLT
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCH
HKTFEAIQFYLLKASMNLAKEKGACPLFNETTYAQGILPIDTYKRDLDKICSEPLHLDWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHH
TLRQDIKQHGLRNSTLSALMPSETSSQISNATNGIEPPRGLISVKASKDGQLKQVVPDFD
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCHH
ELKYNYELLWQMPSNEGYLQLVGIMQKFVDQAISANTNYDPTRFEGRKVPMQTLLKDLLN
HHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKLGVKTLYYHNTRDGASDQHDDITNIEKEDDGCAGGACKI
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]