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The map label for this gene is sppA [H]

Identifier: 117920409

GI number: 117920409

Start: 2336874

End: 2338718

Strand: Reverse

Name: sppA [H]

Synonym: Shewana3_1964

Alternate gene names: 117920409

Gene position: 2338718-2336874 (Counterclockwise)

Preceding gene: 117920411

Following gene: 117920408

Centisome position: 47.04

GC content: 49.05

Gene sequence:

>1845_bases
ATGTCCGCTAACCCATCGTTTTTCAAGAGAATATGTTTATTTATATGGAACACCCTCAACGGGATCCGTAAATTCATACT
CAACCTGATTTTTTTTGGCTTCCTGGCCATCATCCTCATTACTATTGGCAGCAGTGAGGATATTCAGGTCGAAGAGAACT
CAGCATTGGTGCTCAATCTGGCAGGTTCAATTGTCGATCAAAAACAACAGGTCGACCCAATTGAAGCCGCACTTAAACAA
GGCAATAACGGCAGCAGCGATGGTGAAATCCTGCTGTCCGATATTATCTATGTGATTGATAACGCGACGCACGATAACAG
GATCAGCACCATAGTCTTAGACTTAGCCGAGCTGAAACGCGCAGGGATCAGCAAATTACAATCCATTGGCGATGCCCTGA
ATCGTTTTAAAGAAAGCGGCAAAAAAGTGGTGGCAATTGGAAACTATTACGAGCAAAACCAATACTTTCTTGCCAGCTTT
GCCGATACCATTTACCTCAATCCCCAAGGCAGTGTTTCTCTCGATGGCTTGAGTATGTATAACCAATACTTCAAATCCGC
CCTCGAGAAGCTCAAGATAAAGGCACACATTTTCCGCGTCGGCACCTTTAAATCCGCGGTAGAACCTTACATGCGTGACG
ATATGTCGGATGCGGCCAGAGAGGCAAGCAGCGCCCTACTCGCCGATGTTTGGCAGAGTTATACTCAAACGGTTGCAGAA
AACCGCCAAATTGATGCCAATACCTTAGTGCTGGACTCGGCAAGCTACCTCGCGCAACTGGATAAGGCCGAAGGCGACTC
CGCCACTATGGCCATCAATATGAAATGGGTCGATACCCTAGCGACCGATGAAGAGTTCCGTAAAGTCATGCTCGATAGCG
TGGGTAAAGAGAAGTCGGGTGACAGCTTTAAGCAAGTCAGCTTTTACGACTACTTAACCTTAGTGACGCCTTTGCCAAGC
TTTGTTGAGCAAGACAGTGTCGGCATTATCGTTGCCAGCGGCACCATTTTAAATGGCAGCCAACCCGCCGGCCAAATCGG
TGGCGATAGCACTGCAGAGCTGTTACGTAAGGCGCGATTTGATAAACATATCAAAGCGCTGGTACTTAGAGTCGACAGCC
CTGGCGGCAGCGCCTTTGCCTCAGAGCAGATCCGCCAAGAATTACTCGCCCTCAAAGCGGCGGGTAAACCTGTGGTGGTG
AGTATGGGCAGCCTTGCGGCGTCTGGCGGCTACTGGATTTCGGCTAGCGCCGATTATATCTTCGCAACCCCAACTACACT
GACGGGCTCTATCGGGATCTTCGGGATGATCACCACCTTCGAAGATTCACTCGCTAGCTTAGGTATTCATACCGATGGTG
TATCGACCTCTGAATGGGCGGGATTGTCTGTAACTCGTACCCTCTCGCCACAAATCGAATCGGTTATCCAGCGTCATATC
GAGCGTGGTTACTTAGACTTTATCTCACTGGTCGCTAAGGAACGTAAAATGACCTTGGAACAAGTGGATAGCATCGCCCA
AGGCCGTGTGTGGAGTGGTAAAAAAGCCCTAGAGTTAGGTTTAGTGGACGAGCTTGGCGATATCGACCAAGCCGTTGCCA
AAGCCGCTAAATTGGCAGATTTAAGCCTATTCGATACCCGCGTAATTGAACAAGAGCTCACGCCTGAACAGCGTTTTGTG
CAGCAAATGTTTGCCTCTGTTTCGGCCTACTTGCCCGCGTCACTCAGCCATTCAACACTGCTTGAGCAAATGTTGGCGCA
ATGGACGGGCAGCTTAAAGACCATCGCGGCCTTTAACGATCCTAACCATGTGTATGTGTATTGTGACAGCTGTGCGATCA
ACTAA

Upstream 100 bases:

>100_bases
TTGCTAGGGGGTTAAAAGTTAAAATCCTTTACAATTGTGTTTAGCCAGTGAGGGGATTTTCAGTTAGCATTGGCACATTC
GATAAGCAAAGGAGTGGCTA

Downstream 100 bases:

>100_bases
CGCAGACTCAAAATGAAGGACACAAAAGCCCGATTGCCTCGGGCTTTTTTATTGGGTTTATGCCTGCAAAATCGTATAAT
TAGCTCACTTGCAGCTAAGA

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSANPSFFKRICLFIWNTLNGIRKFILNLIFFGFLAIILITIGSSEDIQVEENSALVLNLAGSIVDQKQQVDPIEAALKQ
GNNGSSDGEILLSDIIYVIDNATHDNRISTIVLDLAELKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASF
ADTIYLNPQGSVSLDGLSMYNQYFKSALEKLKIKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAE
NRQIDANTLVLDSASYLAQLDKAEGDSATMAINMKWVDTLATDEEFRKVMLDSVGKEKSGDSFKQVSFYDYLTLVTPLPS
FVEQDSVGIIVASGTILNGSQPAGQIGGDSTAELLRKARFDKHIKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVV
SMGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASLGIHTDGVSTSEWAGLSVTRTLSPQIESVIQRHI
ERGYLDFISLVAKERKMTLEQVDSIAQGRVWSGKKALELGLVDELGDIDQAVAKAAKLADLSLFDTRVIEQELTPEQRFV
QQMFASVSAYLPASLSHSTLLEQMLAQWTGSLKTIAAFNDPNHVYVYCDSCAIN

Sequences:

>Translated_614_residues
MSANPSFFKRICLFIWNTLNGIRKFILNLIFFGFLAIILITIGSSEDIQVEENSALVLNLAGSIVDQKQQVDPIEAALKQ
GNNGSSDGEILLSDIIYVIDNATHDNRISTIVLDLAELKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASF
ADTIYLNPQGSVSLDGLSMYNQYFKSALEKLKIKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAE
NRQIDANTLVLDSASYLAQLDKAEGDSATMAINMKWVDTLATDEEFRKVMLDSVGKEKSGDSFKQVSFYDYLTLVTPLPS
FVEQDSVGIIVASGTILNGSQPAGQIGGDSTAELLRKARFDKHIKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVV
SMGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASLGIHTDGVSTSEWAGLSVTRTLSPQIESVIQRHI
ERGYLDFISLVAKERKMTLEQVDSIAQGRVWSGKKALELGLVDELGDIDQAVAKAAKLADLSLFDTRVIEQELTPEQRFV
QQMFASVSAYLPASLSHSTLLEQMLAQWTGSLKTIAAFNDPNHVYVYCDSCAIN
>Mature_613_residues
SANPSFFKRICLFIWNTLNGIRKFILNLIFFGFLAIILITIGSSEDIQVEENSALVLNLAGSIVDQKQQVDPIEAALKQG
NNGSSDGEILLSDIIYVIDNATHDNRISTIVLDLAELKRAGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFA
DTIYLNPQGSVSLDGLSMYNQYFKSALEKLKIKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAEN
RQIDANTLVLDSASYLAQLDKAEGDSATMAINMKWVDTLATDEEFRKVMLDSVGKEKSGDSFKQVSFYDYLTLVTPLPSF
VEQDSVGIIVASGTILNGSQPAGQIGGDSTAELLRKARFDKHIKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVS
MGSLAASGGYWISASADYIFATPTTLTGSIGIFGMITTFEDSLASLGIHTDGVSTSEWAGLSVTRTLSPQIESVIQRHIE
RGYLDFISLVAKERKMTLEQVDSIAQGRVWSGKKALELGLVDELGDIDQAVAKAAKLADLSLFDTRVIEQELTPEQRFVQ
QMFASVSAYLPASLSHSTLLEQMLAQWTGSLKTIAAFNDPNHVYVYCDSCAIN

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=616, Percent_Identity=46.2662337662338, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1787527, Length=189, Percent_Identity=29.1005291005291, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66984; Mature: 66853

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANPSFFKRICLFIWNTLNGIRKFILNLIFFGFLAIILITIGSSEDIQVEENSALVLNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
AGSIVDQKQQVDPIEAALKQGNNGSSDGEILLSDIIYVIDNATHDNRISTIVLDLAELKR
CHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFADTIYLNPQGSVSLDGLSMY
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEEECCEEEECCCCCEEECHHHHH
NQYFKSALEKLKIKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAE
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NRQIDANTLVLDSASYLAQLDKAEGDSATMAINMKWVDTLATDEEFRKVMLDSVGKEKSG
CCCCCCCEEEEECHHHHHHHHCCCCCCEEEEEEEEEHHHHCCHHHHHHHHHHHCCCCCCC
DSFKQVSFYDYLTLVTPLPSFVEQDSVGIIVASGTILNGSQPAGQIGGDSTAELLRKARF
CCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHH
DKHIKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVSMGSLAASGGYWISASADYI
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEECCCCEE
FATPTTLTGSIGIFGMITTFEDSLASLGIHTDGVSTSEWAGLSVTRTLSPQIESVIQRHI
EECCCCCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ERGYLDFISLVAKERKMTLEQVDSIAQGRVWSGKKALELGLVDELGDIDQAVAKAAKLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCHHHHCHHHHHHHHHHHHHH
LSLFDTRVIEQELTPEQRFVQQMFASVSAYLPASLSHSTLLEQMLAQWTGSLKTIAAFND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
PNHVYVYCDSCAIN
CCEEEEEECCCCCC
>Mature Secondary Structure 
SANPSFFKRICLFIWNTLNGIRKFILNLIFFGFLAIILITIGSSEDIQVEENSALVLNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEE
AGSIVDQKQQVDPIEAALKQGNNGSSDGEILLSDIIYVIDNATHDNRISTIVLDLAELKR
CHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGISKLQSIGDALNRFKESGKKVVAIGNYYEQNQYFLASFADTIYLNPQGSVSLDGLSMY
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEEECCEEEECCCCCEEECHHHHH
NQYFKSALEKLKIKAHIFRVGTFKSAVEPYMRDDMSDAAREASSALLADVWQSYTQTVAE
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NRQIDANTLVLDSASYLAQLDKAEGDSATMAINMKWVDTLATDEEFRKVMLDSVGKEKSG
CCCCCCCEEEEECHHHHHHHHCCCCCCEEEEEEEEEHHHHCCHHHHHHHHHHHCCCCCCC
DSFKQVSFYDYLTLVTPLPSFVEQDSVGIIVASGTILNGSQPAGQIGGDSTAELLRKARF
CCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHH
DKHIKALVLRVDSPGGSAFASEQIRQELLALKAAGKPVVVSMGSLAASGGYWISASADYI
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEECCCCEEEECCCCEE
FATPTTLTGSIGIFGMITTFEDSLASLGIHTDGVSTSEWAGLSVTRTLSPQIESVIQRHI
EECCCCCCCCCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ERGYLDFISLVAKERKMTLEQVDSIAQGRVWSGKKALELGLVDELGDIDQAVAKAAKLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCHHHHCHHHHHHHHHHHHHH
LSLFDTRVIEQELTPEQRFVQQMFASVSAYLPASLSHSTLLEQMLAQWTGSLKTIAAFND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
PNHVYVYCDSCAIN
CCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]