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The map label for this gene is ansA [H]

Identifier: 117920408

GI number: 117920408

Start: 2335749

End: 2336762

Strand: Reverse

Name: ansA [H]

Synonym: Shewana3_1963

Alternate gene names: 117920408

Gene position: 2336762-2335749 (Counterclockwise)

Preceding gene: 117920409

Following gene: 117920396

Centisome position: 47.0

GC content: 48.52

Gene sequence:

>1014_bases
ATGACTAAACGCTCCATTTACGTCGCCTATACGGGCGGCACCATAGGCATGCAAAAAACCGCGAATGGCTTTGCACCGGT
GGCAGGCTTTTTGACCCAATGTGTCCAATCCATGCCCGAGTTTTACCATGATGAAATGCCTGAGTTTGTGATCCATGAGT
ACTGCCCGCTCATCGACTCATCAAACATGGCACCAACCGACTGGCAAATGATCGCCGATGATATCAAGGCCAATTACGAC
AAGTACGATGGCTTTGTGATCCTCCACGGCACAGATACCATGGCCTATACTGCATCGGCCTTATCCTTTATGCTTCAAGG
TCTGTCAAAGCCTGTTATCGTCACCGGCTCTCAAATTCCATTGGCACAGCTGAGATCAGACGGTCAAACCAACCTACTTA
ACTCTTTATATATCGCCGCCAATTATCCGGTGGCTGAAGTGTGTTTATTCTTTAATAACAAACTGTTCCGCGGTAATCGT
TCCACCAAAGCCCATGCCGATGGCTTCGATGCCTTTGCCTCGCCTAACTTCCCCTTACTGCTTGAGGCAGGGATTAAAAT
TAATCTGCGTGCAGGTAAAATTGCCACGCCAAGTGATAAGCCGTTAGAAGTGGCGAATATCAGCCCGCAGCCTGTCGGAG
TGGTGACTCTCTATCCTGGTATTTCGACACAAATTTTTGAGAACATTCTACAGCAACCTGTAAAGGCGCTGATTTTACTA
ACCTTTGGCGTGGGCAATGCACCACAGGATGCGGCATTACTCGATACCCTAAAGCAAGCGGATGAGCGTGGAATTGTGTT
AGTGAACCTAACTCAGTGTTTCCAAGGCAAAGTGAATATGGGCGGTTACGCTACGGGCAATGCCCTCGCCAAAGCGGGGG
TGATCAGTGGTGCAGACATGACCATTGAAGCCGCACTGGCTAAGTTGCATTATCTGCTATCGAAAAACTTAAAGCCGAGC
GAAATTAAAACTGCCATGCTGCAAAACTTGGTTGGTGAGCTCAGCCCCGACTAA

Upstream 100 bases:

>100_bases
AATGAAGGACACAAAAGCCCGATTGCCTCGGGCTTTTTTATTGGGTTTATGCCTGCAAAATCGTATAATTAGCTCACTTG
CAGCTAAGATAACGACAAGA

Downstream 100 bases:

>100_bases
GTATCGAAACGTTACGCATGAAAAAGCCCACATTCGTGGGCTTTTTGTTGTTTAAGCATTAACACAACAATTAGAGTTCG
TCAGCTTTAAACTCAGCAAT

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTKRSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHDEMPEFVIHEYCPLIDSSNMAPTDWQMIADDIKANYD
KYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNR
STKAHADGFDAFASPNFPLLLEAGIKINLRAGKIATPSDKPLEVANISPQPVGVVTLYPGISTQIFENILQQPVKALILL
TFGVGNAPQDAALLDTLKQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLKPS
EIKTAMLQNLVGELSPD

Sequences:

>Translated_337_residues
MTKRSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHDEMPEFVIHEYCPLIDSSNMAPTDWQMIADDIKANYD
KYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNR
STKAHADGFDAFASPNFPLLLEAGIKINLRAGKIATPSDKPLEVANISPQPVGVVTLYPGISTQIFENILQQPVKALILL
TFGVGNAPQDAALLDTLKQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLKPS
EIKTAMLQNLVGELSPD
>Mature_336_residues
TKRSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHDEMPEFVIHEYCPLIDSSNMAPTDWQMIADDIKANYDK
YDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIPLAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRS
TKAHADGFDAFASPNFPLLLEAGIKINLRAGKIATPSDKPLEVANISPQPVGVVTLYPGISTQIFENILQQPVKALILLT
FGVGNAPQDAALLDTLKQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADMTIEAALAKLHYLLSKNLKPSE
IKTAMLQNLVGELSPD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=357, Percent_Identity=42.5770308123249, Blast_Score=257, Evalue=1e-68,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=69.4362017804154, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1789327, Length=277, Percent_Identity=29.2418772563177, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=358, Percent_Identity=43.5754189944134, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI17558278, Length=358, Percent_Identity=43.5754189944134, Blast_Score=273, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI17558280, Length=358, Percent_Identity=43.5754189944134, Blast_Score=273, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI193207372, Length=363, Percent_Identity=42.9752066115703, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24639609, Length=359, Percent_Identity=42.3398328690808, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24645530, Length=356, Percent_Identity=43.2584269662921, Blast_Score=271, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36332; Mature: 36201

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHDEMPEFVIHEYCPLIDS
CCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SNMAPTDWQMIADDIKANYDKYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRSTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHHEEEEECCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGIKINLRAGKIATPSDKPLEVANISPQPVGVVTLYPGISTQIFENILQQPVKALILL
EECCEEEEEECCCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TFGVGNAPQDAALLDTLKQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADM
EECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCH
TIEAALAKLHYLLSKNLKPSEIKTAMLQNLVGELSPD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKRSIYVAYTGGTIGMQKTANGFAPVAGFLTQCVQSMPEFYHDEMPEFVIHEYCPLIDS
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SNMAPTDWQMIADDIKANYDKYDGFVILHGTDTMAYTASALSFMLQGLSKPVIVTGSQIP
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
LAQLRSDGQTNLLNSLYIAANYPVAEVCLFFNNKLFRGNRSTKAHADGFDAFASPNFPLL
HHHHHCCCCHHHHHHEEEEECCCHHHHHHHHCCEEEECCCCCCCCCCCCHHCCCCCCCEE
LEAGIKINLRAGKIATPSDKPLEVANISPQPVGVVTLYPGISTQIFENILQQPVKALILL
EECCEEEEEECCCCCCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TFGVGNAPQDAALLDTLKQADERGIVLVNLTQCFQGKVNMGGYATGNALAKAGVISGADM
EECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCH
TIEAALAKLHYLLSKNLKPSEIKTAMLQNLVGELSPD
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]