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The map label for this gene is oprM [H]

Identifier: 117920363

GI number: 117920363

Start: 2279346

End: 2280815

Strand: Direct

Name: oprM [H]

Synonym: Shewana3_1918

Alternate gene names: 117920363

Gene position: 2279346-2280815 (Clockwise)

Preceding gene: 117920362

Following gene: 117920364

Centisome position: 45.84

GC content: 53.74

Gene sequence:

>1470_bases
ATGAATAAACATCTATTAATGGCCTTTACGGCAATGAGCTTGCTTGGCGGATGCAGTATGTCCCCTGATTACACACCGCC
AAAGGTGAAAGTCAGTGAGCTGTATTTAAATGCGGCGACTGAGGGCGTGAGCCAAGAGGCCTTGGCTTACCAAGCTTGGT
GGCGTCAGTTTAACGATCCTCAGCTCAATGCGCTGGTGGCAAAGGCGCAGCAGCAAAATATAAGTCTGCAGATCGCCAGT
GAGCGTGTGCGCGCCGCACGGGCCTATCAGGCGGCGGTGTCATCGTTAAAAGTGCCGACCGTTTCGGTGGGCGCGGGGTA
CCTCGATATGCGCATCAGTGAAAACGATCCGCTGATGGGCAGTGCGGTTGGAGGCATAGAACTACCCGCTGGCTTGGGTG
GCGGCAACCTTAAGTTGATGAATCGTGACCCGAACAGTACAACCGTGGGCGCGAGCATTGCTTGGGAGGCGGATTTGTTT
GGCCGAATAGCGAGTTTAACTCAGGCCGCAGAAATCCGCGCCGAGCAGGCGCAATTGTACCGCGCTAATCTGACCACCCT
AATAACGGCGGAGGTTATCGATAACTATCTGCAATACCGTGGTACGCAGGAGCGGATGGCGATTGCAAGGCAAAACATTG
CCGAACAAGCCGAAGTCTTGGCCTTGGTGGAATCCTTGGAGCGCAATGGTTATGGCTCGCCGCTGGATGTCGCTAATGCC
AAGGCGGCGCTTGCCGCCAGCGAGGCCATGTTACCCATGTTAAGTTCGGCCGAGCAGGCGCATTTGCATCGACTGGCGAT
TTTGCTCGGGGAAAGCCTCAAGGAGACGTCAGGACGGTTAAGCGCCGCGGCGTTACCCGCAATGACGGAGTTAATCCCAA
CGGGCGTACCTTCAGAGTTACTGACACGCCGTTTAGATATTGCCATTGCCGAGCGTGAAATGGCCGCTAAAAACCAAGAG
CTTGGGGCGGCGATTGCGGCTAAGTATCCGAGCTTTTTCTTAACGGGGGCACCGGGATTATCGGCCGATCATATTGACGA
TCTCTTCTCATCGGATTCGCTTACTTGGGCTTTAGGCGCGGGCGTGAGCTGGAATGTCTTCGATGGTGGCCGCACTCAAG
CCATGGTGGAAATACAGGAAGCGGGTTTTAAAACTGCGGCGCTTAGCTACCAGCAGACGGTGAATAACGCCATCAATGAA
GTCGAAACCGTGCTAAAGCATTATGGCAATAATCAGCAATATCATCGCTTTATTGCAAAGGCAGAGGCGCAATCTGAGGT
TGCGGTACAAAAAGCGACTTCGCTGTATCGCGCCGGTTTAGAAAATCACTTGAGCGTGTTAACTGCGCAGAATGTGAAAA
ACAATATGCAGGATGCGGAAGTGTTAGCCCGCCTACAAACGGCATCGAGTGTGGTGTCGCTTTATAAGGCATTGGGCGGC
GACTGGACTCTGGCAAACACCGCGGATTAA

Upstream 100 bases:

>100_bases
GACTCTTTTATCGTCGAGAAGAGCGTGAGGAAGATGCATTACTCGATGCACAAACGGACAACACTCCATCGGTTATTAGC
AGTGCCAAAGGGGCCTAAAC

Downstream 100 bases:

>100_bases
AGATTGTATGAGGGGGCTCTTGCCGTTAGGTGGTACTACAGCGGCAGGGGCGCCTGATTTCATGACGATTATTCGTTGCT
AATAATCCTCTTTAATCGTG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNKHLLMAFTAMSLLGGCSMSPDYTPPKVKVSELYLNAATEGVSQEALAYQAWWRQFNDPQLNALVAKAQQQNISLQIAS
ERVRAARAYQAAVSSLKVPTVSVGAGYLDMRISENDPLMGSAVGGIELPAGLGGGNLKLMNRDPNSTTVGASIAWEADLF
GRIASLTQAAEIRAEQAQLYRANLTTLITAEVIDNYLQYRGTQERMAIARQNIAEQAEVLALVESLERNGYGSPLDVANA
KAALAASEAMLPMLSSAEQAHLHRLAILLGESLKETSGRLSAAALPAMTELIPTGVPSELLTRRLDIAIAEREMAAKNQE
LGAAIAAKYPSFFLTGAPGLSADHIDDLFSSDSLTWALGAGVSWNVFDGGRTQAMVEIQEAGFKTAALSYQQTVNNAINE
VETVLKHYGNNQQYHRFIAKAEAQSEVAVQKATSLYRAGLENHLSVLTAQNVKNNMQDAEVLARLQTASSVVSLYKALGG
DWTLANTAD

Sequences:

>Translated_489_residues
MNKHLLMAFTAMSLLGGCSMSPDYTPPKVKVSELYLNAATEGVSQEALAYQAWWRQFNDPQLNALVAKAQQQNISLQIAS
ERVRAARAYQAAVSSLKVPTVSVGAGYLDMRISENDPLMGSAVGGIELPAGLGGGNLKLMNRDPNSTTVGASIAWEADLF
GRIASLTQAAEIRAEQAQLYRANLTTLITAEVIDNYLQYRGTQERMAIARQNIAEQAEVLALVESLERNGYGSPLDVANA
KAALAASEAMLPMLSSAEQAHLHRLAILLGESLKETSGRLSAAALPAMTELIPTGVPSELLTRRLDIAIAEREMAAKNQE
LGAAIAAKYPSFFLTGAPGLSADHIDDLFSSDSLTWALGAGVSWNVFDGGRTQAMVEIQEAGFKTAALSYQQTVNNAINE
VETVLKHYGNNQQYHRFIAKAEAQSEVAVQKATSLYRAGLENHLSVLTAQNVKNNMQDAEVLARLQTASSVVSLYKALGG
DWTLANTAD
>Mature_489_residues
MNKHLLMAFTAMSLLGGCSMSPDYTPPKVKVSELYLNAATEGVSQEALAYQAWWRQFNDPQLNALVAKAQQQNISLQIAS
ERVRAARAYQAAVSSLKVPTVSVGAGYLDMRISENDPLMGSAVGGIELPAGLGGGNLKLMNRDPNSTTVGASIAWEADLF
GRIASLTQAAEIRAEQAQLYRANLTTLITAEVIDNYLQYRGTQERMAIARQNIAEQAEVLALVESLERNGYGSPLDVANA
KAALAASEAMLPMLSSAEQAHLHRLAILLGESLKETSGRLSAAALPAMTELIPTGVPSELLTRRLDIAIAEREMAAKNQE
LGAAIAAKYPSFFLTGAPGLSADHIDDLFSSDSLTWALGAGVSWNVFDGGRTQAMVEIQEAGFKTAALSYQQTVNNAINE
VETVLKHYGNNQQYHRFIAKAEAQSEVAVQKATSLYRAGLENHLSVLTAQNVKNNMQDAEVLARLQTASSVVSLYKALGG
DWTLANTAD

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=495, Percent_Identity=26.4646464646465, Blast_Score=129, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52444; Mature: 52444

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHLLMAFTAMSLLGGCSMSPDYTPPKVKVSELYLNAATEGVSQEALAYQAWWRQFNDP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
QLNALVAKAQQQNISLQIASERVRAARAYQAAVSSLKVPTVSVGAGYLDMRISENDPLMG
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCC
SAVGGIELPAGLGGGNLKLMNRDPNSTTVGASIAWEADLFGRIASLTQAAEIRAEQAQLY
CCCCCEECCCCCCCCCEEEEECCCCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RANLTTLITAEVIDNYLQYRGTQERMAIARQNIAEQAEVLALVESLERNGYGSPLDVANA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KAALAASEAMLPMLSSAEQAHLHRLAILLGESLKETSGRLSAAALPAMTELIPTGVPSEL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHH
LTRRLDIAIAEREMAAKNQELGAAIAAKYPSFFLTGAPGLSADHIDDLFSSDSLTWALGA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEEECC
GVSWNVFDGGRTQAMVEIQEAGFKTAALSYQQTVNNAINEVETVLKHYGNNQQYHRFIAK
CEEEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AEAQSEVAVQKATSLYRAGLENHLSVLTAQNVKNNMQDAEVLARLQTASSVVSLYKALGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
DWTLANTAD
CEEECCCCC
>Mature Secondary Structure
MNKHLLMAFTAMSLLGGCSMSPDYTPPKVKVSELYLNAATEGVSQEALAYQAWWRQFNDP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
QLNALVAKAQQQNISLQIASERVRAARAYQAAVSSLKVPTVSVGAGYLDMRISENDPLMG
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCC
SAVGGIELPAGLGGGNLKLMNRDPNSTTVGASIAWEADLFGRIASLTQAAEIRAEQAQLY
CCCCCEECCCCCCCCCEEEEECCCCCCEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RANLTTLITAEVIDNYLQYRGTQERMAIARQNIAEQAEVLALVESLERNGYGSPLDVANA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KAALAASEAMLPMLSSAEQAHLHRLAILLGESLKETSGRLSAAALPAMTELIPTGVPSEL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHH
LTRRLDIAIAEREMAAKNQELGAAIAAKYPSFFLTGAPGLSADHIDDLFSSDSLTWALGA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEEECC
GVSWNVFDGGRTQAMVEIQEAGFKTAALSYQQTVNNAINEVETVLKHYGNNQQYHRFIAK
CEEEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AEAQSEVAVQKATSLYRAGLENHLSVLTAQNVKNNMQDAEVLARLQTASSVVSLYKALGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
DWTLANTAD
CEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]