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The map label for this gene is frmA [H]

Identifier: 117920284

GI number: 117920284

Start: 2178680

End: 2179819

Strand: Direct

Name: frmA [H]

Synonym: Shewana3_1837

Alternate gene names: 117920284

Gene position: 2178680-2179819 (Clockwise)

Preceding gene: 117920282

Following gene: 117920285

Centisome position: 43.82

GC content: 50.61

Gene sequence:

>1140_bases
ATGTCGAACGAAAAACCACAGTTTATCAAGTCAAAGGCCGCCGTGGCCTGGGGCCCGGGACAGCCGCTCAAGATTGAAGA
AGTCGATGTGATGTTACCAAAAGCCGGCGAAGTGTTAGTTCGCATCGTGGCGACTGGGGTTTGCCATACTGATGCGTTTA
CCTTAAGTGGTGACGATCCAGAGGGCGTGTTCCCAGCGATCCTCGGCCATGAGGGCGGTGGTATTGTTGAGCAAGTGGGC
GAAGGCGTGACCAGCGTGCAGGTGGGCGACCATGTGATCCCTCTCTACACGCCTGAGTGTGGTGAATGTAAATTCTGTTT
ATCGGGTAAAACAAACCTGTGCCAGAAGATCCGTGCAACCCAAGGCAAAGGTTTGATGCCAGATGGCACCACGCGTTTCT
ATAAAGATGGCCAGCCCATTTTCCATTACATGGGTTGTTCTACCTTCTCTGAATACACGGTATTACCTGAAATTTCATTG
GCTAAGGTTAATAAAACTGCGCCATTAGAAGAAATCTGTTTGCTGGGTTGTGGTGTGACAACAGGTATGGGCGCTGTGAT
GAACACGGCTAAAGTGGAAGAAGGCGCGACCGTGGCGATTTTCGGCCTTGGCGGTATCGGTCTTTCGGCCATTATTGGTG
CGACCATGGCTAAAGCCAGCCGGATCATTGCCATCGATATCAATGAATCTAAATTTGAACTTGCCCGTAAATTAGGGGCA
ACCGATTGTATTAACCCGAAACATTTCGACAAACCCATTCAAGAGGTGATTGTCGAAATGACCGATGGCGGCGTGGATTA
CTCCTTCGAATGTATCGGTAATGTCAATGTGATGCGCAGCGCGCTCGAGTGTTGCCACAAGGGCTGGGGCGAGTCAGTGA
TCATTGGGGTTGCGGGCGCAGGGCAGGAGATTTCTACCCGTCCATTCCAACTGGTGACGGGCCGCGTATGGCGTGGTTCT
GCCTTCGGTGGCGTGAAAGGACGCTCGCAATTACCTAAAATCGTTGAACAATACCTAGCGGGTGAGTTTAAGCTCGATGA
CTTTATCACCCACACTATGGGACTTGAGCAAGTTAACGAAGCCTTCGACTTGATGCATGAAGGCAAGAGCATTCGCAGCG
TGATCCATTTCGATAAATAA

Upstream 100 bases:

>100_bases
TTAAGCTTATCCGTCCACCTCAATCTATGCTTACAAGCGAGTAGGGCTAGGGTAAGCCGTAAATCGATAAGCCCATTGAT
TAAAAGAAGGAAAGACAAGC

Downstream 100 bases:

>100_bases
GCGCTCAGTTTGCCATCACAGCAGCAGATAACCGCTAACGGCTTATCTGCTGCTTATGCGTTTTAAGCTTGTGATAGGGC
CTTGAACGGCAAGCTAAACG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVG
EGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYKDGQPIFHYMGCSTFSEYTVLPEISL
AKVNKTAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGA
TDCINPKHFDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGS
AFGGVKGRSQLPKIVEQYLAGEFKLDDFITHTMGLEQVNEAFDLMHEGKSIRSVIHFDK

Sequences:

>Translated_379_residues
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVG
EGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYKDGQPIFHYMGCSTFSEYTVLPEISL
AKVNKTAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGA
TDCINPKHFDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGS
AFGGVKGRSQLPKIVEQYLAGEFKLDDFITHTMGLEQVNEAFDLMHEGKSIRSVIHFDK
>Mature_378_residues
SNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVGE
GVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYKDGQPIFHYMGCSTFSEYTVLPEISLA
KVNKTAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGAT
DCINPKHFDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSA
FGGVKGRSQLPKIVEQYLAGEFKLDDFITHTMGLEQVNEAFDLMHEGKSIRSVIHFDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=375, Percent_Identity=65.0666666666667, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI34577061, Length=373, Percent_Identity=52.2788203753351, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4501929, Length=373, Percent_Identity=51.4745308310992, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4501933, Length=373, Percent_Identity=50.9383378016086, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI71565152, Length=377, Percent_Identity=51.1936339522546, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=48.5094850948509, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI4501939, Length=363, Percent_Identity=48.7603305785124, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=49.3224932249323, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=49.0566037735849, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=70.4607046070461, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790045, Length=371, Percent_Identity=26.9541778975741, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787863, Length=359, Percent_Identity=26.1838440111421, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082125, Length=374, Percent_Identity=26.2032085561497, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.8698060941828, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081918, Length=228, Percent_Identity=34.6491228070175, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786825, Length=272, Percent_Identity=27.9411764705882, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788075, Length=367, Percent_Identity=26.158038147139, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788407, Length=225, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI226510992, Length=356, Percent_Identity=21.3483146067416, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=26.0752688172043, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=383, Percent_Identity=59.7911227154047, Blast_Score=487, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71997431, Length=385, Percent_Identity=59.4805194805195, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=59.4752186588921, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17562584, Length=371, Percent_Identity=28.8409703504043, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=382, Percent_Identity=28.0104712041885, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=362, Percent_Identity=26.5193370165746, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=58.6666666666667, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6323099, Length=376, Percent_Identity=28.4574468085106, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=29.1005291005291, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=369, Percent_Identity=28.9972899728997, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.3736263736264, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319258, Length=267, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=289, Percent_Identity=24.9134948096886, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737895, Length=379, Percent_Identity=63.8522427440633, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17137530, Length=253, Percent_Identity=30.8300395256917, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17737897, Length=269, Percent_Identity=28.2527881040892, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40562; Mature: 40431

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDP
CCCCCCCHHHHCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCC
EGVFPAILGHEGGGIVEQVGEGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRAT
CCCCHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHHC
QGKGLMPDGTTRFYKDGQPIFHYMGCSTFSEYTVLPEISLAKVNKTAPLEEICLLGCGVT
CCCCCCCCCCCHHCCCCCCEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHCCHH
TGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGA
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCC
TDCINPKHFDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGA
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
GQEISTRPFQLVTGRVWRGSAFGGVKGRSQLPKIVEQYLAGEFKLDDFITHTMGLEQVNE
CCCCCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
AFDLMHEGKSIRSVIHFDK
HHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
SNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDP
CCCCCCHHHHCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCC
EGVFPAILGHEGGGIVEQVGEGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRAT
CCCCHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHHC
QGKGLMPDGTTRFYKDGQPIFHYMGCSTFSEYTVLPEISLAKVNKTAPLEEICLLGCGVT
CCCCCCCCCCCHHCCCCCCEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHCCHH
TGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGA
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCC
TDCINPKHFDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGA
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
GQEISTRPFQLVTGRVWRGSAFGGVKGRSQLPKIVEQYLAGEFKLDDFITHTMGLEQVNE
CCCCCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
AFDLMHEGKSIRSVIHFDK
HHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]