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The map label for this gene is puuB [H]

Identifier: 117920282

GI number: 117920282

Start: 2176226

End: 2177515

Strand: Direct

Name: puuB [H]

Synonym: Shewana3_1835

Alternate gene names: 117920282

Gene position: 2176226-2177515 (Clockwise)

Preceding gene: 117920277

Following gene: 117920284

Centisome position: 43.77

GC content: 52.48

Gene sequence:

>1290_bases
ATGTCTGCTAAACGTTGCGCGTCTTATTACAATGCCACCATCAAGCAAGAGTCTAATTATCCTGAACTTGAAGGCGATAT
TCGGGTCGATGTGGCCATTATCGGCGGTGGATTTACCGGTGTGGCAACGGCGGTCGAATTGGCCGAAAAGGGTTACCAAG
TGGCGTTACTTGAGGCCAATAAGATTGCCTGGGGGGCCACAGGGCGAAATGGCGGCCAAGTGACGGGGAGTTTGTCCGGC
GATGCCGCCATGACCAAGCAATTGCGGCGTCAGCTTGGTCAGGAGGCTGAAGATTATGTGTGGAACCTTCGTTGGCGCGG
CCACGATATTATCAAAAACAGAGTGGCGAAGTATGGCATTGATTGTGATTTAAAATTTGGCCATATCCAAACGGCGTATC
GCCCCGCACATATGGTCGAGTTACAGCAAATGTTTGATGAAGCTAATCGCCGTGGCATGGGGGAATTGATGACCTTAGTG
CCAGCGCAGGAGATGCCCGCCTACTTAGCTTCGCCCTTGTACCACGGGGGGCTGGTCAATCGGCGGAATATGCATCTGCA
TTCGGTGAATTTATGTCTTGGTGAGGCACGAGCCGCGGCTTCCTTAGGCGCTAAGATTTTTGAACATTCGCAGGTGCTGG
ATATTCAGCAAGGAGAGCTCGCAACTGTGGTCACGCCAAAGGGCAGGGTGAAGGCAAACAGTGTGCTTATCGCCGGTAAT
GCCTATCACAAATTAGGCCGACCTCAGCTGCGGGGCATGTTATTTCCGGCCTCCCTTGGTAATTGCGCGACGGCACAATT
GCCAGAAGATTTAGCTAAGCAACTCAATCCGCAGGATTTAGCCGTGTATGATTGCCGCTTTGTACTGGATTATTACCGCT
TAACCGCCGATAACCGTTTGATGTTTGGGGGCGGCACCAATTACAGCGGCCGCGATCCTAAAAACGTTGCCGCCGAGCTG
CGTCCGGCGATTGAACGCACTTTCCCCCAGTTAAAGGGCGTCGACATTGAATTTGCCTGGGCTGGCATGGCGGGGATAGT
GATTAACCGTATTCCCCAGCTGGGCAAGATTGCGCCGAATATCTTCTATTGTCAGGGATATTCGGGCCATGGCGTGGCGA
CTTCCCATATTATGGCCGAGATCATGGGGCAGGCGATCGATGGTCAAATGCGAGAGTTCGATCTCTTCGCCTCCATGCGT
CATATCCGGATCCCATTGAATGAATGGTTTGGTAATCAGGCCCTTGCGCTAGGGATGTTGTACTACACCTTGAGGGAAAA
CTGGCGCTAA

Upstream 100 bases:

>100_bases
CTTCTGTTGACCACGATATTAGCTTAGATATACTCAAAAATTCTGAACAAATGTAAAAAATATAAAACAAAAACAGCTTA
AATCTGTTCAAAGGATCCCT

Downstream 100 bases:

>100_bases
ATAGCCAGTAATCCGTCAAGCTCAAGCGCGTTATTGGTCGCAAGATTGGGTGATTTGTTACTTTGGTTCGCTGCAATGGA
CTCGTTACTACGCATCCTCC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSAKRCASYYNATIKQESNYPELEGDIRVDVAIIGGGFTGVATAVELAEKGYQVALLEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQLGQEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYRPAHMVELQQMFDEANRRGMGELMTLV
PAQEMPAYLASPLYHGGLVNRRNMHLHSVNLCLGEARAAASLGAKIFEHSQVLDIQQGELATVVTPKGRVKANSVLIAGN
AYHKLGRPQLRGMLFPASLGNCATAQLPEDLAKQLNPQDLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKIAPNIFYCQGYSGHGVATSHIMAEIMGQAIDGQMREFDLFASMR
HIRIPLNEWFGNQALALGMLYYTLRENWR

Sequences:

>Translated_429_residues
MSAKRCASYYNATIKQESNYPELEGDIRVDVAIIGGGFTGVATAVELAEKGYQVALLEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQLGQEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYRPAHMVELQQMFDEANRRGMGELMTLV
PAQEMPAYLASPLYHGGLVNRRNMHLHSVNLCLGEARAAASLGAKIFEHSQVLDIQQGELATVVTPKGRVKANSVLIAGN
AYHKLGRPQLRGMLFPASLGNCATAQLPEDLAKQLNPQDLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKIAPNIFYCQGYSGHGVATSHIMAEIMGQAIDGQMREFDLFASMR
HIRIPLNEWFGNQALALGMLYYTLRENWR
>Mature_428_residues
SAKRCASYYNATIKQESNYPELEGDIRVDVAIIGGGFTGVATAVELAEKGYQVALLEANKIAWGATGRNGGQVTGSLSGD
AAMTKQLRRQLGQEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYRPAHMVELQQMFDEANRRGMGELMTLVP
AQEMPAYLASPLYHGGLVNRRNMHLHSVNLCLGEARAAASLGAKIFEHSQVLDIQQGELATVVTPKGRVKANSVLIAGNA
YHKLGRPQLRGMLFPASLGNCATAQLPEDLAKQLNPQDLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAAELR
PAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKIAPNIFYCQGYSGHGVATSHIMAEIMGQAIDGQMREFDLFASMRH
IRIPLNEWFGNQALALGMLYYTLRENWR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=427, Percent_Identity=37.7049180327869, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47454; Mature: 47323

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKRCASYYNATIKQESNYPELEGDIRVDVAIIGGGFTGVATAVELAEKGYQVALLEAN
CCHHHHHHHHCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQLGQEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYRPAHMVELQQMFDEANRRGMGELMTLVPAQEMPAYLASPLYHGGLVN
CCEEEECCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCHHHCCCCCC
RRNMHLHSVNLCLGEARAAASLGAKIFEHSQVLDIQQGELATVVTPKGRVKANSVLIAGN
CCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEECCEEEECC
AYHKLGRPQLRGMLFPASLGNCATAQLPEDLAKQLNPQDLAVYDCRFVLDYYRLTADNRL
HHHHCCCHHHCCEECCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKIAPN
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHCCCCC
IFYCQGYSGHGVATSHIMAEIMGQAIDGQMREFDLFASMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC
>Mature Secondary Structure 
SAKRCASYYNATIKQESNYPELEGDIRVDVAIIGGGFTGVATAVELAEKGYQVALLEAN
CHHHHHHHHCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQLGQEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYRPAHMVELQQMFDEANRRGMGELMTLVPAQEMPAYLASPLYHGGLVN
CCEEEECCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCHHHCCCCCC
RRNMHLHSVNLCLGEARAAASLGAKIFEHSQVLDIQQGELATVVTPKGRVKANSVLIAGN
CCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEECCEEEECC
AYHKLGRPQLRGMLFPASLGNCATAQLPEDLAKQLNPQDLAVYDCRFVLDYYRLTADNRL
HHHHCCCHHHCCEECCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKIAPN
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHCCCCC
IFYCQGYSGHGVATSHIMAEIMGQAIDGQMREFDLFASMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]