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The map label for this gene is yebU [H]

Identifier: 117920217

GI number: 117920217

Start: 2083636

End: 2085168

Strand: Reverse

Name: yebU [H]

Synonym: Shewana3_1770

Alternate gene names: 117920217

Gene position: 2085168-2083636 (Counterclockwise)

Preceding gene: 117920219

Following gene: 117920207

Centisome position: 41.94

GC content: 47.49

Gene sequence:

>1533_bases
ATGGCAAAAGCCAAGGGAGAAAATCCCAGCCCAGTAAAGGATTATGATATACTTGCCGCAGTTGATACACAGCCTAATCC
CGTTATGGTTCAATTAAATCAAAATTTTATCAATACTATTGCCCAAGAACTGCCCGCCCATCTCTCAATGGATGACTTTA
TTGCGGCCTGTTCGAGACCACTACGTCGCTCGATTCGCGTCAATACCTTAAAGACCAGCAGTGAAGACTTTAAGCGCTTA
ATGCAGCCCAAAGGATGGACCTTTGAGCCCATTCCATGGTGTGAGGATGGCTTCTGGATAAGCTACGATGAAGAAGAGCA
GCTCGGCAACGCCCTAGAGCATATTCAGGGATTGTTCTATATCCAAGAAGCCAGCTCCATGTTGCCCCCCACAGCGCTAT
TTACCCCAAATGCGGATTGGCAATGTGTATTAGATTTAGCCTCTGCGCCCGGCTCTAAGACCACTCAAATGGCGGCATTA
ATGAATAATCAAGGCTTATTAGTCGCCAATGAATATTCCGCCAGCCGTGTCAAAGTGCTCCATGCCAATGTGCTACGCAT
GGGTGCCAGTCACTGTGCCCTGACCCACTTTGATGGACGGGTATTTGGCGAGTATCTGTATGAAAGTTTTGATGCGGTAT
TGATTGATGCTCCCTGCGGCGGTGAAGGCACAGTTCGTAAAGATGCGGACGCGTTAAAATCTTGGTCCCTTGATGAAGTA
ATCGAAATTAGCGAAACCCAAAAGGCGTTAATCGAATCCGCCTTTTTGGCGTTAAAACCGGGTGGCAGTTTGGTGTATTC
CACTTGCACCCTTAATCGGCACGAAAACCAAGGCGTGTGCGAATATTTACAGCAAACCTATGGCGATGCGGTGCAATTTG
AGTCCTTAAGCCAATTGTTTGATGGCGCTGAAAAAGCCACTACGCCAGAAGGCTTTTTGCACGTTTGGCCGCAAATTTAC
GACAGTGAAGGCTTTTTTGTCGCTAAGCTGACTAAAACTCGCTCCGTGCCCCGTCTGCAACCTGAGCCTAAATTACAAAA
GAATTTCCCCTTTACGGAGGCGAGTGCCAAACAAGCCAAAGCCATTCAAGCCTATTTTGCCGATGATCTGGGTATCGAAC
TGCCCGATGATCTCATTATGGTGCGCGACGATGAGTTTTGGCTCTTCCCCCATGAATTTAGAGACTTTATTGGCAAAATG
CGCTTTCAGCGGATTGGGGTGAAGCTTGCCGACCACAGTAAGCATGGCTTTAAAGTCCGCCATGAAGCCATTATCGCACT
TGCGGGTAAAGCGCTTAAAGCTGGGGCAAAAAATGGCGCGAAAGTCGTCGAAGTAAGCGATGAGCAGGCGAAAGAATATT
TGATGGGACGAGATATTCCCCTCGATACGGCGGGCAAAGCTCAAGGCGAAGTGATTGTCTGTTATGGCGGTGCGCCTTTA
GGCATGGCTAAACATTTAGGCAATAAATTAAAAAACAGCCTGCCCAGAGACTTAGTAAAAGATAAAGTCCTATTGCTACC
ACCACAGGCATAA

Upstream 100 bases:

>100_bases
GATGTGAGGAAAAACACTAACCACACCCGATTTAGCACTCTGCCCCTTCCTTATTCTTATTCATGCTTTGTCTTTGTGTT
GCTTTAATGACTCGTTTGGG

Downstream 100 bases:

>100_bases
TGGTTATAAATCGGCGATTTTTTAGCGAGAAATAACCTAAAAAACGAGGCGGAGAAAGTGTCACTCACACTAAACTCAAC
TACACTGAGTCTATTGTAAA

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MAKAKGENPSPVKDYDILAAVDTQPNPVMVQLNQNFINTIAQELPAHLSMDDFIAACSRPLRRSIRVNTLKTSSEDFKRL
MQPKGWTFEPIPWCEDGFWISYDEEEQLGNALEHIQGLFYIQEASSMLPPTALFTPNADWQCVLDLASAPGSKTTQMAAL
MNNQGLLVANEYSASRVKVLHANVLRMGASHCALTHFDGRVFGEYLYESFDAVLIDAPCGGEGTVRKDADALKSWSLDEV
IEISETQKALIESAFLALKPGGSLVYSTCTLNRHENQGVCEYLQQTYGDAVQFESLSQLFDGAEKATTPEGFLHVWPQIY
DSEGFFVAKLTKTRSVPRLQPEPKLQKNFPFTEASAKQAKAIQAYFADDLGIELPDDLIMVRDDEFWLFPHEFRDFIGKM
RFQRIGVKLADHSKHGFKVRHEAIIALAGKALKAGAKNGAKVVEVSDEQAKEYLMGRDIPLDTAGKAQGEVIVCYGGAPL
GMAKHLGNKLKNSLPRDLVKDKVLLLPPQA

Sequences:

>Translated_510_residues
MAKAKGENPSPVKDYDILAAVDTQPNPVMVQLNQNFINTIAQELPAHLSMDDFIAACSRPLRRSIRVNTLKTSSEDFKRL
MQPKGWTFEPIPWCEDGFWISYDEEEQLGNALEHIQGLFYIQEASSMLPPTALFTPNADWQCVLDLASAPGSKTTQMAAL
MNNQGLLVANEYSASRVKVLHANVLRMGASHCALTHFDGRVFGEYLYESFDAVLIDAPCGGEGTVRKDADALKSWSLDEV
IEISETQKALIESAFLALKPGGSLVYSTCTLNRHENQGVCEYLQQTYGDAVQFESLSQLFDGAEKATTPEGFLHVWPQIY
DSEGFFVAKLTKTRSVPRLQPEPKLQKNFPFTEASAKQAKAIQAYFADDLGIELPDDLIMVRDDEFWLFPHEFRDFIGKM
RFQRIGVKLADHSKHGFKVRHEAIIALAGKALKAGAKNGAKVVEVSDEQAKEYLMGRDIPLDTAGKAQGEVIVCYGGAPL
GMAKHLGNKLKNSLPRDLVKDKVLLLPPQA
>Mature_509_residues
AKAKGENPSPVKDYDILAAVDTQPNPVMVQLNQNFINTIAQELPAHLSMDDFIAACSRPLRRSIRVNTLKTSSEDFKRLM
QPKGWTFEPIPWCEDGFWISYDEEEQLGNALEHIQGLFYIQEASSMLPPTALFTPNADWQCVLDLASAPGSKTTQMAALM
NNQGLLVANEYSASRVKVLHANVLRMGASHCALTHFDGRVFGEYLYESFDAVLIDAPCGGEGTVRKDADALKSWSLDEVI
EISETQKALIESAFLALKPGGSLVYSTCTLNRHENQGVCEYLQQTYGDAVQFESLSQLFDGAEKATTPEGFLHVWPQIYD
SEGFFVAKLTKTRSVPRLQPEPKLQKNFPFTEASAKQAKAIQAYFADDLGIELPDDLIMVRDDEFWLFPHEFRDFIGKMR
FQRIGVKLADHSKHGFKVRHEAIIALAGKALKAGAKNGAKVVEVSDEQAKEYLMGRDIPLDTAGKAQGEVIVCYGGAPLG
MAKHLGNKLKNSLPRDLVKDKVLLLPPQA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=301, Percent_Identity=32.2259136212625, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI76150623, Length=301, Percent_Identity=32.2259136212625, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI32698918, Length=237, Percent_Identity=32.9113924050633, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI301336155, Length=301, Percent_Identity=26.2458471760797, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI39995082, Length=301, Percent_Identity=26.2458471760797, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI11545785, Length=252, Percent_Identity=32.5396825396825, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40316918, Length=197, Percent_Identity=35.0253807106599, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI270288818, Length=324, Percent_Identity=28.0864197530864, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI8922322, Length=324, Percent_Identity=28.0864197530864, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI270288816, Length=341, Percent_Identity=27.8592375366569, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI23199998, Length=319, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081985, Length=474, Percent_Identity=53.7974683544304, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI2367212, Length=217, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=300, Percent_Identity=31, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25143471, Length=198, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71998419, Length=304, Percent_Identity=25.9868421052632, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=231, Percent_Identity=27.7056277056277, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324268, Length=288, Percent_Identity=34.0277777777778, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319447, Length=307, Percent_Identity=26.3843648208469, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI22024126, Length=304, Percent_Identity=31.25, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355201, Length=287, Percent_Identity=27.5261324041812, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19920494, Length=210, Percent_Identity=30.4761904761905, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24668781, Length=157, Percent_Identity=35.031847133758, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356513, Length=157, Percent_Identity=35.031847133758, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 56529; Mature: 56398

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKGENPSPVKDYDILAAVDTQPNPVMVQLNQNFINTIAQELPAHLSMDDFIAACSRP
CCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCHHHHHHHHCHH
LRRSIRVNTLKTSSEDFKRLMQPKGWTFEPIPWCEDGFWISYDEEEQLGNALEHIQGLFY
HHHCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHEE
IQEASSMLPPTALFTPNADWQCVLDLASAPGSKTTQMAALMNNQGLLVANEYSASRVKVL
EEHHHHCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCEEHH
HANVLRMGASHCALTHFDGRVFGEYLYESFDAVLIDAPCGGEGTVRKDADALKSWSLDEV
HHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHH
IEISETQKALIESAFLALKPGGSLVYSTCTLNRHENQGVCEYLQQTYGDAVQFESLSQLF
HHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
DGAEKATTPEGFLHVWPQIYDSEGFFVAKLTKTRSVPRLQPEPKLQKNFPFTEASAKQAK
CCHHHCCCCCCCEEECCCEECCCCEEEEEEECCCCCCCCCCCCCHHCCCCCCCCHHHHHH
AIQAYFADDLGIELPDDLIMVRDDEFWLFPHEFRDFIGKMRFQRIGVKLADHSKHGFKVR
HHHHHHHCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHHHHCCEEECCCCCCCEEH
HEAIIALAGKALKAGAKNGAKVVEVSDEQAKEYLMGRDIPLDTAGKAQGEVIVCYGGAPL
HHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCH
GMAKHLGNKLKNSLPRDLVKDKVLLLPPQA
HHHHHHHHHHHHHCCHHHHHCCEEEECCCC
>Mature Secondary Structure 
AKAKGENPSPVKDYDILAAVDTQPNPVMVQLNQNFINTIAQELPAHLSMDDFIAACSRP
CCCCCCCCCCCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCHHHHHHHHCHH
LRRSIRVNTLKTSSEDFKRLMQPKGWTFEPIPWCEDGFWISYDEEEQLGNALEHIQGLFY
HHHCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHEE
IQEASSMLPPTALFTPNADWQCVLDLASAPGSKTTQMAALMNNQGLLVANEYSASRVKVL
EEHHHHCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCEEHH
HANVLRMGASHCALTHFDGRVFGEYLYESFDAVLIDAPCGGEGTVRKDADALKSWSLDEV
HHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHH
IEISETQKALIESAFLALKPGGSLVYSTCTLNRHENQGVCEYLQQTYGDAVQFESLSQLF
HHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
DGAEKATTPEGFLHVWPQIYDSEGFFVAKLTKTRSVPRLQPEPKLQKNFPFTEASAKQAK
CCHHHCCCCCCCEEECCCEECCCCEEEEEEECCCCCCCCCCCCCHHCCCCCCCCHHHHHH
AIQAYFADDLGIELPDDLIMVRDDEFWLFPHEFRDFIGKMRFQRIGVKLADHSKHGFKVR
HHHHHHHCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHHHHCCEEECCCCCCCEEH
HEAIIALAGKALKAGAKNGAKVVEVSDEQAKEYLMGRDIPLDTAGKAQGEVIVCYGGAPL
HHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCH
GMAKHLGNKLKNSLPRDLVKDKVLLLPPQA
HHHHHHHHHHHHHCCHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA