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The map label for this gene is metG [H]

Identifier: 117920207

GI number: 117920207

Start: 2073887

End: 2075917

Strand: Reverse

Name: metG [H]

Synonym: Shewana3_1760

Alternate gene names: 117920207

Gene position: 2075917-2073887 (Counterclockwise)

Preceding gene: 117920217

Following gene: 117920206

Centisome position: 41.75

GC content: 49.34

Gene sequence:

>2031_bases
ATGGCAACTTCACAACGTAAAATTCTCGTGACCAGCGCACTTCCCTACGCGAACGGACCGATTCATTTAGGTCACATGCT
GGAATACATCCAGACGGATATCTGGTCGCGTTATCAAAAGCTTCGTGGACATGAGTGCCACTATATTTGTGCCGATGACG
CTCACGGCACACCGATCATGCTCAAGGCGCAGCAATTAGGCATGGCGCCTGAAGAAATGATCGCTCAAGTCAACAAAGAG
CATCAGCAGGATTTCGCCGATTTTAATATCGCCTTCGATAATTATCACAGCACCCACAGTGAAGAAAATCGCGTGCTGGC
GAGCGATATTTACTTAAAACTGCGCGCTAATGGCTATATCAAGAGCAAGAGCATTTCGCAGTTATTTGACCCTGAAAAGT
CGATGTTCCTACCAGACCGCTTCGTAAAAGGCACCTGCCCTAAATGTAAGTCACCGGATCAATACGGCGACAACTGTGAT
GCCTGCGGCGCAACTTACAGCCCAACTGAGCTGATTAACCCTAAATCGGCAGTCTCTGGCGCGACGCCTGTGATGAAGGA
CACTGAGCACTTTTTCTTCGACTTACCCGCCTTCGAAGACATGCTCAAAGAGTGGACTCGTTCGGGTGCACTGCAAACCG
AAATGGCCAACAAACTCGACGAATGGTTCGAACAGGGTCTGCAGCAATGGGATATCACCCGCGATGCGCCTTACTTTGGC
TTTGAGATCCCAGATGCACCGGGCAAATACTTCTACGTATGGTTAGATGCGCCTATTGGCTATATGGGCTCATTCAAAAA
CCTCTGTGACAAACATCCCGAGCTGAGCTTTGACGAATTTTGGGCTAAAGATTCTAAGGCCGAAGTTTACCATTTTATCG
GTAAAGACATCGTTTACTTCCACAGCCTGTTCTGGCCAGCCATGCTCCATGGCTCGGGCTACCGTCAACCTAACAGCGTT
TACGCCCACGGTTATGTGACAGTAAACGGCGCTAAGATGTCTAAATCTAAGGGCACCTTTATTAAGGCGCGCACCTATTT
AGACCACTTAGATCCTGAATACTTACGTTACTACTACGCGGCCAAGCTGAGCAGCCGTATCGACGATCTCGACCTGAATT
TAGAAGACTTCGTTCAGCGCGTAAACTCAGACTTAGTGGGTAAGTTGGTGAATCTAGCGTCTCGCACCGCGGGCTTTATC
ACTAAGCGTTTCGATGGCAAGTTGGCCAAGATTGCCGACACCACGCTTACGGATGCGTTTTTAGCTAAGCAAGAGCAAAT
CGCCGAGTTCTATGAAACCCGCGAATACGGTAAAGCAATGCGCGAAATCATGGCATTGGCCGATATCGCCAACGGTTTTG
TCGCCGACGCAGCACCTTGGCAGTTAGTGAAGCAAGACGATCAACAGGAAACGGCGCACCAAGTGTGCTCGAATGCCCTG
AATCTGTTCCGCATTCTGGTCACTTACTTAAAACCTGTGTTGCCACGTTTAGCGTTGGATGTTGAAGCCTTCTTCCAACA
GACCTTAACTTGGGATGCGCTAAACCAAGATATGGCTGGCCATGAAATTGCACCATTCAAAGCCATGATGCAACGTGTTG
AATTAGAAAAGGTTAACGCTATGGTTGCCGACTCTAAAGAAAACCTGCAAGCCACGAGCGAGCCAGAAGCACCTAAGGGC
CCATTGGCGACCGATCCAATTAGCGACACCATCAACTTTGAAGATTTCGCTAAAATCGATCTGCGTATTGCGCGTATCGT
CAAAGCCGAGCATGTGGCCGAAGCCGACAAACTGTTAAAACTGCAATTGGATATCGGCGGCGAGACCCGTCAGGTATTTG
CAGGCATTAAATCGGCCTACTCGCCAGAAGATCTCGAAGGTAAGCTGACTGTTATGGTCGCCAACCTTGCGCCACGTAAG
ATGCGTTTTGGCATGTCAGAAGGCATGGTACTGGCAGCAGGCCCAGGTGGTAGCGATTTGTGGATTTTAGAACCCCACGA
AGGCGCACAACCTGGTATGCGTGTGAAGTAA

Upstream 100 bases:

>100_bases
GCAGATTTAACCCAATCGACTCAGGTTTTGATGAAAATCATCCTGTAATCGGTTAGTATCTCTACCATTATTTTTGGCCC
ACCACGATATTTGAGACAAG

Downstream 100 bases:

>100_bases
GCTAGGCTAAACACGAGTCAAGCTACTGCAAAATTGATATAAGGCCGCATTTAATGCGGCCTTATTTGTTGGTAAAAACA
TCCCGTTACGCTTTTCTGGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNKE
HQQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCD
ACGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAKIADTTLTDAFLAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNAL
NLFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNAMVADSKENLQATSEPEAPKG
PLATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRK
MRFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK

Sequences:

>Translated_676_residues
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNKE
HQQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCD
ACGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAKIADTTLTDAFLAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNAL
NLFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNAMVADSKENLQATSEPEAPKG
PLATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRK
MRFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK
>Mature_675_residues
ATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGMAPEEMIAQVNKEH
QQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDA
CGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFGF
EIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSVY
AHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFIT
KRFDGKLAKIADTTLTDAFLAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNALN
LFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNAMVADSKENLQATSEPEAPKGP
LATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKM
RFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=628, Percent_Identity=28.0254777070064, Blast_Score=258, Evalue=2e-68,
Organism=Homo sapiens, GI24308436, Length=516, Percent_Identity=26.5503875968992, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1788432, Length=679, Percent_Identity=67.59941089838, Blast_Score=996, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=37.037037037037, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=528, Percent_Identity=30.3030303030303, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=22.8515625, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=23.3415233415233, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=560, Percent_Identity=28.9285714285714, Blast_Score=246, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=594, Percent_Identity=23.0639730639731, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24655311, Length=558, Percent_Identity=30.1075268817204, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI21358483, Length=512, Percent_Identity=25, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76241; Mature: 76110

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIM
CCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMAPEEMIAQVNKEHQQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEEEEECCCEE
KSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDACGATYSPTELINPKSAVSG
ECCHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFG
CCCCCCCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLHGSGYRQPNSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFITKRFDGKLAKIADTTLTDAF
HHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
MVADSKENLQATSEPEAPKGPLATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLK
HHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKMRFGMSEGMVLAAGPGGSDL
EEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCCE
WILEPHEGAQPGMRVK
EEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIM
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGMAPEEMIAQVNKEHQQDFADFNIAFDNYHSTHSEENRVLASDIYLKLRANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEEEEECCCEE
KSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDACGATYSPTELINPKSAVSG
ECCHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMKDTEHFFFDLPAFEDMLKEWTRSGALQTEMANKLDEWFEQGLQQWDITRDAPYFG
CCCCCCCCCHHEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCDKHPELSFDEFWAKDSKAEVYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAMLHGSGYRQPNSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSSRIDDLDLNLEDFVQRVNSDLVGKLVNLASRTAGFITKRFDGKLAKIADTTLTDAF
HHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LAKQEQIAEFYETREYGKAMREIMALADIANGFVADAAPWQLVKQDDQQETAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLALDVEAFFQQTLTWDALNQDMAGHEIAPFKAMMQRVELEKVNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
MVADSKENLQATSEPEAPKGPLATDPISDTINFEDFAKIDLRIARIVKAEHVAEADKLLK
HHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKMRFGMSEGMVLAAGPGGSDL
EEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCCE
WILEPHEGAQPGMRVK
EEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA