| Definition | Shewanella sp. ANA-3 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008577 |
| Length | 4,972,204 |
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The map label for this gene is topA [H]
Identifier: 117920176
GI number: 117920176
Start: 2035692
End: 2038376
Strand: Direct
Name: topA [H]
Synonym: Shewana3_1729
Alternate gene names: 117920176
Gene position: 2035692-2038376 (Clockwise)
Preceding gene: 117920174
Following gene: 117920178
Centisome position: 40.94
GC content: 49.09
Gene sequence:
>2685_bases ATGGAGCCAATTTCCACCTTCTTGAGATTTTTTGGCGCAAATCAAACTATGGGTAAATCGCTAGTTATTGTCGAATCACC GGCCAAAGCTAAGACTATTAATAAATATCTTGGCAAAGAATTCATCGTTAAATCGAGCGTGGGGCATATCCGTGACCTAC CAACCTCTTCTTCATCTGACGGCAATGAAAAGGTGAAATCCGCCGCCGAAGTGAAGAAGATGTCGCCAGAAGAAAAGGCG CGATATAAAAAGGTGAAAGAGCACCAGGCGCTGGTGTCGCGTATGGGCGTTAACCCTGAAAAGGGCTGGGCGGCTAAATA TCAAATCCTACCGGGTAAAGAGAAAGTCGTTAAAGAGCTGCAAGCCTTAGCCGATTCTGCTGACCAAATCTATCTTGCAA CGGACTTAGACCGTGAAGGGGAAGCCATCGCCTGGCATTTGCAAGAGGTGATTGGTGGCGATCCTTCACGGTATCAACGC GTTGTATTTAACGAAATCACCAAATCTGCGATTCAAGAAGCCTTTAGTAAACCTTCTTCCCTCGACACTAATATGGTGAA TGCCCAGCAGGCGCGACGCTTTTTAGACCGTGTCGTCGGCTTTATGGTGTCTCCACTGTTGTGGAAAAAGGTTGCCCGCG GCTTATCCGCAGGACGGGTGCAATCCGTGGCCGTGCGTCTTGTGGTGGAGCGTGAAAGCGAAATCAAAGCCTTCGTACCT GAAGAGTTTTGGGATGTGCACGCACAGCTGAATACGCCTGCGAGCGAAGCGCTACGCATGGAAGTGGTAAAGTACTTAGA CTCAGCCTTCGAGCCCACAAACGAGCAGCAAGCGTTAGCCGCCGTTCAAGCCTTATCCTCTGCCAGCTTTAAAGTGATCG CTCGTGAAGATAAAGCGACTCAAAGTAAACCTTCGGCTCCTTTTATTACCTCAACCCTACAACAAGCGGCCAGTACTCGC CTAGGCTTTGGGGTGAAGAAAACCATGATGATGGCCCAGCGCTTGTACGAAGCGGGTCACATCACTTATATGCGTACCGA TTCGACAAACTTAAGCCAAGAAGCGTTAGACAGTGTGCGTGAGATGATCGGCAAGGAATACGGCGCCAAGTATTTACCTG CCGAACCAATTCGCTACGGCTCTAAGGAAGGCGCACAAGAGGCGCACGAAGCGATTCGTCCATCAAACGTGAAGGTCGAA GCGGCTGCATTAACCGATATGGAACGTGATGCACAGCGTCTCTACGAGCTGATTTGGCGTCAGTTTGTGTCATGCCAGAT GACACCTGCCCAATACGATGCGACCAAACTCACGGTTAAGGCGGGTGACTATGAACTGAAAGCGACGGGACGTATCCTGC GCTTTGACGGTTGGACTCGTGTTCAAACCGCATTGAAAAAGAAAAATGAAGAAGACAATACCTTGCCATCGGTGAACGAA GGTGATCTGTTGTCTCTGGTTGAACTAGAGCCTAAGCAACACTTTACTAAGCCGCCTGCCAGATACAGCGAAGCGTCATT GGTAAAAGAATTAGAGAAGCGTGGTATCGGTCGTCCTTCAACCTACGCCACGATTATTTCGACGATTCAAGAGCGGGGTT ATGTCAAAGTTGAAAACCGCCGTTTCTACGCAGAGAAAATGGGCGAGATTGTCAGTGAGCGTTTAGTCGGTAGTTTTGCT GAGCTGATGAGCTACGACTTTACCGCCAGCATGGAACAGACCCTTGACGATGTCGCCAGTGGTGAGCTGGATTGGAAAAA GGTTCTCGATGGTTTCTATGCCGACTTCACTAAACAACTCGAGAAGGCGGAATTGGCACCAGAAGAAGGCGGTATGCGCC TCAATCAAATGGTGATGACCAATATTAAGTGTCCGACTTGCGGTCGCCCCATGGGGATCCGTACCGGCACAACTGGGGTG TTCCTGGGTTGTTCGGGTTACGAGTTGCCACCGAAAGAGCGCTGCAAAACCACCATGAACTTAACGCCAGGCATTGAAGC GATTAGCGACAGTGAAGATGCTGAAACCGATGCGCTACGCGCCAAGCATCGTTGCGGCATTTGTGGCACTGCGATGGACA GCTATCTTATCGACGAAAAGCGTAAGCTGCATGTTTGCGGTAATAACCCCATCTGTGAGGGTTATGAAGTCGAAGAAGGA CAATTTAAGATCAAAGGTTACGAAGGTCCATTAATTGAATGTGACCGCTGTGGCCATGATATGGAGCTGAAAAACGGCCG TTTCGGTAAATATTTTGGTTGTACTAACGAGGAATGTAAGAATACTCGTAAGTTACTGAAAAACGGTGAAGCTGCGCCAC CAAAAGAAGATCCTATTTATCTGCCAGAGCTGAAATGCAGCAAGTCTGATGCGCATTTTGTGCTGCGTGACGGTGCGGCA GGGATTTTCTTGGCAGCCAGTACTTTCCCTAAATCTCGGGAAACCCGGGCGCCACTGGTTGAAGAGTTAGTGAAATATCG CGAGCTATTGTGGCCAAAATATCAGTATCTTGCCGATGCTCCTATCGCCGATGATGAGGGGAATAAAGCGGCGGTTAAGT TTAGCCGTAAGACCAAAGAGCAATATGTGGCGACCGAAGTTGATGGTAAAGCAACCGGATGGTCGGCGCATTATGTGAAT GGTAAATGGGTGACTGAATCGACGGCGAAGAAAGCTAAGAAGTAA
Upstream 100 bases:
>100_bases ATATTATTTTTTTACATTTTTTCAACTTTGATTAAAAAGTGACTCGCTGGTACATATAAAAGAGGGATTAGCTTGAAACT CTCAATGCAACCCTCTATAT
Downstream 100 bases:
>100_bases CCTGATCTGAATAGGGTTTAGGATCCTTTTCTTCGTGGTTATAAATGGGTTCACTTAGGTGAGCCCATTTTGTTATCTAG GGTTCTTACAATCGCCGATT
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 894; Mature: 894
Protein sequence:
>894_residues MEPISTFLRFFGANQTMGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSSSDGNEKVKSAAEVKKMSPEEKA RYKKVKEHQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQR VVFNEITKSAIQEAFSKPSSLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVP EEFWDVHAQLNTPASEALRMEVVKYLDSAFEPTNEQQALAAVQALSSASFKVIAREDKATQSKPSAPFITSTLQQAASTR LGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALDSVREMIGKEYGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVKVE AAALTDMERDAQRLYELIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRILRFDGWTRVQTALKKKNEEDNTLPSVNE GDLLSLVELEPKQHFTKPPARYSEASLVKELEKRGIGRPSTYATIISTIQERGYVKVENRRFYAEKMGEIVSERLVGSFA ELMSYDFTASMEQTLDDVASGELDWKKVLDGFYADFTKQLEKAELAPEEGGMRLNQMVMTNIKCPTCGRPMGIRTGTTGV FLGCSGYELPPKERCKTTMNLTPGIEAISDSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEG QFKIKGYEGPLIECDRCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAA GIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADAPIADDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVN GKWVTESTAKKAKK
Sequences:
>Translated_894_residues MEPISTFLRFFGANQTMGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSSSDGNEKVKSAAEVKKMSPEEKA RYKKVKEHQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQR VVFNEITKSAIQEAFSKPSSLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVP EEFWDVHAQLNTPASEALRMEVVKYLDSAFEPTNEQQALAAVQALSSASFKVIAREDKATQSKPSAPFITSTLQQAASTR LGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALDSVREMIGKEYGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVKVE AAALTDMERDAQRLYELIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRILRFDGWTRVQTALKKKNEEDNTLPSVNE GDLLSLVELEPKQHFTKPPARYSEASLVKELEKRGIGRPSTYATIISTIQERGYVKVENRRFYAEKMGEIVSERLVGSFA ELMSYDFTASMEQTLDDVASGELDWKKVLDGFYADFTKQLEKAELAPEEGGMRLNQMVMTNIKCPTCGRPMGIRTGTTGV FLGCSGYELPPKERCKTTMNLTPGIEAISDSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEG QFKIKGYEGPLIECDRCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAA GIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADAPIADDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVN GKWVTESTAKKAKK >Mature_894_residues MEPISTFLRFFGANQTMGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSSSDGNEKVKSAAEVKKMSPEEKA RYKKVKEHQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQR VVFNEITKSAIQEAFSKPSSLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVP EEFWDVHAQLNTPASEALRMEVVKYLDSAFEPTNEQQALAAVQALSSASFKVIAREDKATQSKPSAPFITSTLQQAASTR LGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALDSVREMIGKEYGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVKVE AAALTDMERDAQRLYELIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRILRFDGWTRVQTALKKKNEEDNTLPSVNE GDLLSLVELEPKQHFTKPPARYSEASLVKELEKRGIGRPSTYATIISTIQERGYVKVENRRFYAEKMGEIVSERLVGSFA ELMSYDFTASMEQTLDDVASGELDWKKVLDGFYADFTKQLEKAELAPEEGGMRLNQMVMTNIKCPTCGRPMGIRTGTTGV FLGCSGYELPPKERCKTTMNLTPGIEAISDSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEG QFKIKGYEGPLIECDRCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIYLPELKCSKSDAHFVLRDGAA GIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADAPIADDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVN GKWVTESTAKKAKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI4507635, Length=500, Percent_Identity=28.4, Blast_Score=163, Evalue=7e-40, Organism=Homo sapiens, GI10835218, Length=577, Percent_Identity=25.4766031195841, Blast_Score=127, Evalue=4e-29, Organism=Escherichia coli, GI1787529, Length=870, Percent_Identity=71.3793103448276, Blast_Score=1299, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=561, Percent_Identity=24.9554367201426, Blast_Score=121, Evalue=2e-28, Organism=Caenorhabditis elegans, GI32563869, Length=604, Percent_Identity=24.3377483443709, Blast_Score=127, Evalue=2e-29, Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=25.7309941520468, Blast_Score=121, Evalue=2e-27, Organism=Saccharomyces cerevisiae, GI6323263, Length=497, Percent_Identity=25.5533199195171, Blast_Score=110, Evalue=1e-24, Organism=Drosophila melanogaster, GI24640096, Length=502, Percent_Identity=26.0956175298805, Blast_Score=123, Evalue=5e-28, Organism=Drosophila melanogaster, GI24585251, Length=590, Percent_Identity=24.9152542372881, Blast_Score=121, Evalue=2e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 99792; Mature: 99792
Theoretical pI: Translated: 8.05; Mature: 8.05
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEPISTFLRFFGANQTMGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSSSD CCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCC GNEKVKSAAEVKKMSPEEKARYKKVKEHQALVSRMGVNPEKGWAAKYQILPGKEKVVKEL CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHH QALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQEAFSKPSS HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC LDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVP CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHCCC EEFWDVHAQLNTPASEALRMEVVKYLDSAFEPTNEQQALAAVQALSSASFKVIAREDKAT HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC QSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALDSVR CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH EMIGKEYGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVKVEAAALTDMERDAQRLYELIWR HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHH QFVSCQMTPAQYDATKLTVKAGDYELKATGRILRFDGWTRVQTALKKKNEEDNTLPSVNE HHHCCCCCCCCCCCEEEEEECCCEEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCCCC GDLLSLVELEPKQHFTKPPARYSEASLVKELEKRGIGRPSTYATIISTIQERGYVKVENR CCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECC RFYAEKMGEIVSERLVGSFAELMSYDFTASMEQTLDDVASGELDWKKVLDGFYADFTKQL CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH EKAELAPEEGGMRLNQMVMTNIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCKTTMN HHHHCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECC LTPGIEAISDSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEG CCCCCHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEECCC QFKIKGYEGPLIECDRCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIY EEEEECCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEE LPELKCSKSDAHFVLRDGAAGIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADA CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCC PIADDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVNGKWVTESTAKKAKK CCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCEEEEECCEEECCHHHHHCCC >Mature Secondary Structure MEPISTFLRFFGANQTMGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSSSD CCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCC GNEKVKSAAEVKKMSPEEKARYKKVKEHQALVSRMGVNPEKGWAAKYQILPGKEKVVKEL CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHH QALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQEAFSKPSS HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC LDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVP CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHCCC EEFWDVHAQLNTPASEALRMEVVKYLDSAFEPTNEQQALAAVQALSSASFKVIAREDKAT HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC QSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEALDSVR CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH EMIGKEYGAKYLPAEPIRYGSKEGAQEAHEAIRPSNVKVEAAALTDMERDAQRLYELIWR HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHH QFVSCQMTPAQYDATKLTVKAGDYELKATGRILRFDGWTRVQTALKKKNEEDNTLPSVNE HHHCCCCCCCCCCCEEEEEECCCEEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCCCC GDLLSLVELEPKQHFTKPPARYSEASLVKELEKRGIGRPSTYATIISTIQERGYVKVENR CCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECC RFYAEKMGEIVSERLVGSFAELMSYDFTASMEQTLDDVASGELDWKKVLDGFYADFTKQL CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH EKAELAPEEGGMRLNQMVMTNIKCPTCGRPMGIRTGTTGVFLGCSGYELPPKERCKTTMN HHHHCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECC LTPGIEAISDSEDAETDALRAKHRCGICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEG CCCCCHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEECCC QFKIKGYEGPLIECDRCGHDMELKNGRFGKYFGCTNEECKNTRKLLKNGEAAPPKEDPIY EEEEECCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEE LPELKCSKSDAHFVLRDGAAGIFLAASTFPKSRETRAPLVEELVKYRELLWPKYQYLADA CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCC PIADDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVNGKWVTESTAKKAKK CCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCEEEEECCEEECCHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]