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The map label for this gene is cysB [H]

Identifier: 117920178

GI number: 117920178

Start: 2039604

End: 2040578

Strand: Direct

Name: cysB [H]

Synonym: Shewana3_1731

Alternate gene names: 117920178

Gene position: 2039604-2040578 (Clockwise)

Preceding gene: 117920176

Following gene: 117920183

Centisome position: 41.02

GC content: 46.97

Gene sequence:

>975_bases
ATGAAACTGCAACAGCTCAGGTATATTGCTGAGGTCGTCAAACACAACCTTAACGTATCGGCCACCGCGGAAAATTTATA
TACATCGCAACCTGGGATCAGTAAGCAAGTTCGCATGTTGGAAGATGAATTAGGCATTCAAATCTTCGGTCGCAGCGGTA
AACATTTGACCCACGTGACACCCGCCGGACAACAGGTGATAAATATCGCCAATGACATCTTAGGCAAAGTTGAAAGCATC
AAAAAAGTCTCCGAGGAATACACTAAACCTGATCAGGGGGAATTGAATATCGCGACCACGGATACGCAGGCGCGGTATGC
GTTACCCCATATCATTCGCCAGTTCATTGACCGTTATCCTAAAGTCAACCTGCATATGCACCAAGGCACGCCATCGCAGA
TCAGCGAACAAGCGGCGCGTGGCGATGCTGACTTTGCGATTGCCACCGAAGCGATGCACTTGTATTCCGACTTAATCATG
TTGCCTTGTTACCATTGGAATCGCTCGGTTGTGGTTCCCAAAGATCATCCCTTGGCGACCCGTACCCATATCAGTATTGA
AGATTTAGCCCGTTTCCCATTAGTCACTTATGTGTTTGGTTTTGACCGTGCCTCGGAAATTGAAAAAGCCTTTAACCGCG
CAAACCTCGAGCCGCGGGTGGTCTTTAGTGCGACCAGCGCCGATGTGCTGAAAACCTATGTACGTTTAGGCTTAGGAGTC
GGGGTGATAGCCTCGATGGCGATTGACCCTAATATCGATAAAGACTTAGTGGCTATCGATGCGGGCCATTTATTTGCCCA
CAGCACGACTAAGATTGGTTTTAGAAAAGGCAGTTTCTTACGTAGTTATATGTATGATTTTATTCAACACTTTGCACCGC
ATTTGACTCGCGATGTCGTTGAAAAGGCGGTGGCATTACGCGATCAGCAATTGATTGATGAAATGTTTGCCGATAAAAAC
TTGCCAATGCGTTGA

Upstream 100 bases:

>100_bases
CTTTAATTTTTACTATATACTATAACGATTGATTATAGCTGATACTGAGTTGTGCCATTTTCGCCAATCCAATTCAGCCT
GTTTGAGACAAGGACATAAG

Downstream 100 bases:

>100_bases
TTCGCGGTTGTTAGAGACAATAAAAAATGCCGCAATGAATGCGGCATTTTTTATGCGAAATTGACAGCAGTCACGGCCGA
ATTAGGTCAAATCCACTTTA

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPHIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKDHPLATRTHISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADKN
LPMR

Sequences:

>Translated_324_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPHIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKDHPLATRTHISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADKN
LPMR
>Mature_324_residues
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGQQVINIANDILGKVESI
KKVSEEYTKPDQGELNIATTDTQARYALPHIIRQFIDRYPKVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIM
LPCYHWNRSVVVPKDHPLATRTHISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLGV
GVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVVEKAVALRDQQLIDEMFADKN
LPMR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=71.1180124223602, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=37.3801916932907, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=26.5734265734266, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI157672245, Length=248, Percent_Identity=28.2258064516129, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=26.8965517241379, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=187, Percent_Identity=26.2032085561497, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789204, Length=226, Percent_Identity=23.0088495575221, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=26.8456375838926, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790208, Length=231, Percent_Identity=23.8095238095238, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36481; Mature: 36481

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVSEEYTKPDQGELNIATTDTQARYALPHIIRQFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSVVVPKDHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCC
RTHISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLGV
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCH
GVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVV
HHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLQQLRYIAEVVKHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGQQVINIANDILGKVESIKKVSEEYTKPDQGELNIATTDTQARYALPHIIRQFIDRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
KVNLHMHQGTPSQISEQAARGDADFAIATEAMHLYSDLIMLPCYHWNRSVVVPKDHPLAT
CEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEECCCCCCCCC
RTHISIEDLARFPLVTYVFGFDRASEIEKAFNRANLEPRVVFSATSADVLKTYVRLGLGV
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCH
GVIASMAIDPNIDKDLVAIDAGHLFAHSTTKIGFRKGSFLRSYMYDFIQHFAPHLTRDVV
HHHHHHHCCCCCCCCEEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKAVALRDQQLIDEMFADKNLPMR
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]