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The map label for this gene is pflB [H]

Identifier: 117920003

GI number: 117920003

Start: 1811301

End: 1813583

Strand: Reverse

Name: pflB [H]

Synonym: Shewana3_1555

Alternate gene names: 117920003

Gene position: 1813583-1811301 (Counterclockwise)

Preceding gene: 117920006

Following gene: 117920002

Centisome position: 36.47

GC content: 49.15

Gene sequence:

>2283_bases
ATGACCGACAAAACTGAACTGTTTTCCAACGCATGGGAAGGTTTTACCCCTGGCGATTGGAAATCTGAAGTCAATGTACG
TGACTTTATTCAACAAAACTATGCTCCCTATGAAGGTGACGAGTCATTCCTAGCCGGTGCCACCGAAGCCACAACTCAGT
TGTGGGACAAAGTGATGGAAGGCATCAAGCAAGAAAACCGCACCCACGCGCCAGTTGATTTCGATACTAAGATGGTCTCT
ACCATCACTTCCCATGACGCGGGTTATATCAATAAAGATTTAGAAACTATCGTTGGTTTACAAACCGATGCGCCGCTCAA
GCGCGCTATGTTACCTAACGGTGGTATTCGCATGGTTGAAGGCTCATGTGCCGCTTACAACCGCGAACTCGACGCCGATG
TAAAATACATCTACTCAGAACTGCGTAAAACACACAACCAAGGCGTATTCGACGTCTACACCCCAGAAATCATGGCGTGC
CGTAAATCCGGCGTATTAACTGGTTTACCCGATGCCTATGGTCGTGGTCGTATTATCGGTGACTATCGCCGCGTAGCGCT
CTACGGTATCGACTTCTTAATGAAGGATAAATTTGCCCAGTTCAGCTCACTGCAAGCGCAGTTCGAAGCCGGCGAAGATT
TATCTAACGTTATCCAACTGCGTGAAGAAATTGCCGAGCAGCACCGCGCTCTAGGTCAAATGAAGACCATGGCGGCTAAA
TACGGTTTCGATATTTCTCGCCCAGCCGCTAACGCCAAAGAAGCGATTCAATGGACATATTTTGGCTACTTAGCTGCGGT
TAAGAGCCAAAACGGCGCAGCTATGTCTTTAGGCCGTACCTCATCTTTCCTCGATATCTACATCGAGCGCGATCTGAAAA
ACGGCACCATCACAGAGCAACAAGCTCAAGAAATGATTGACCATTTCGTGATGAAACTGCGTATGGTGCGTTTCCTACGT
ACTCCAGAATACGATGAGTTATTCTCAGGCGATCCTATTTGGGCAACTGAATCTATCGGCGGTATGGGCTTAGATGGCCG
TACACTAGTGACCAAATCCAGCTTCCGTTTCTTAAACACCTTATACACTATGGGCCCAAGCCCAGAGCCAAACATCACTG
TGCTCTGGTCTGACAAACTGCCTGTAGGCTTTAAAAAGTACTGCGCGAAAGTGTCTATCGACACCAGCTCTATCCAGTAC
GAAAACGATGACTTAATGCGCCCAGATTTCCAATCTGACGATTACGCTATCGCTTGCTGCGTGAGCCCAATGGTTGTGGG
TAAACACATGCAGTTCTTCGGCGCCCGTGCAAACTTGGCAAAAACCATGTTGTATGCGATCAACGGCGGTGTTGACGAAA
AACTGAAAATCCAAATCGCGCCAAAAGCCGCCCCAATCACCGACGAAGTGTTGAACTTCGATGACGTGATGAATCGTTTA
GACGGCCTGATGGATTGGTTAGCGACGCAATATGTCACTGCGCTGAACTCTATTCACTACATGCACGACAAGTACTCCTA
CGAAGCTGCGCTAATGGCGCTGCACGACAGAGACGTTCGTCGTACCATGGCTTGTGGTATCGCAGGGCTTTCAATCGCGG
CCGACTCACTGTCTGCCATCAAGTACGCTCAAGTTAAACCTGTGCGTGATGAAAACGGCATTGCCGTCGACTTTGAGATC
AGCGGTGATTATCCAAAATTTGGTAACAACGACCCACGCGTCGACGATATCGCCTGTGACTTAGTTGAACGTTTTATGTC
GAAGATCCGCGACCGTAAAATGTACCGCAACGCTATCCCAACTCAGTCAATTCTGACCATTACCTCTAACGTGGTTTATG
GTAAGAAAACCGGTAACACGCCAGACGGTCGCCGTTCAGGTGCGCCATTTGCACCGGGTGCAAACCCAATGCACGGCCGT
GATGAGAAAGGTGCGATTGCCTCGTTAACCTCAGTGGCAAAACTGCCTTTTGCCCACGCACAGGACGGTATCTCATACAC
CTTCTCCATCGTGCCTAATGCCTTAGGTAAAGATGAAGACGGTCGCCGTACTAACCTCGCGGCGTTGATGGACGGATATT
TCGCCCACAACGAAGGACACGAAGGCGGTCAACACTTGAACGTTAACGTGATGAACCGTGAAATGCTTGAGGATGCGGTC
GTTAACCCTGACAAGTATCCGCAACTGACCATCCGGGTATCGGGTTACGCAGTGCGCTTTAACTCTTTGACCCCAGAGCA
GCAGCAAGACGTGATCACACGTACTTTCACAAAAGGTCTGTAA

Upstream 100 bases:

>100_bases
GAAGGGAGCTTTAATCAGGAAATAGCGATTGGCCTATACTCAACATGGTTTGCCCCATGAGCGCCAATGACCTTTATCTC
AACCAAAAGGTATTAGTACG

Downstream 100 bases:

>100_bases
GCTAAATTAGGTAAGGCTGTAGTACAATGTGCTACAGCCTCACTTATTTCAACAGGAGTCACAATGGCAGTTACCGGTCG
GATCCACTCAGTGGAATCCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVMEGIKQENRTHAPVDFDTKMVS
TITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVEGSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMAC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAK
YGFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQY
ENDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRL
DGLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAQVKPVRDENGIAVDFEI
SGDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAV
VNPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Sequences:

>Translated_760_residues
MTDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVMEGIKQENRTHAPVDFDTKMVS
TITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVEGSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMAC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAK
YGFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQQAQEMIDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQY
ENDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRL
DGLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAQVKPVRDENGIAVDFEI
SGDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAV
VNPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
>Mature_759_residues
TDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVMEGIKQENRTHAPVDFDTKMVST
ITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVEGSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMACR
KSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAKY
GFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQQAQEMIDHFVMKLRMVRFLRT
PEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNTLYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQYE
NDDLMRPDFQSDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRLD
GLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAIKYAQVKPVRDENGIAVDFEIS
GDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRD
EKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAVV
NPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=81.8421052631579, Blast_Score=1340, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=755, Percent_Identity=75.364238410596, Blast_Score=1236, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=581, Percent_Identity=27.710843373494, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1790388, Length=667, Percent_Identity=25.0374812593703, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=83.3333333333333, Blast_Score=109, Evalue=8e-25,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84750; Mature: 84619

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVME
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKQENRTHAPVDFDTKMVSTITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEEEEE
GSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMACRKSGVLTGLPDAYGRGRIIG
CCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQ
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQYENDDLMRPDFQSDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCHHHHHHHH
DGLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAQVKPVRDENGIAVDFEISGDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIP
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHC
VNPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDKTELFSNAWEGFTPGDWKSEVNVRDFIQQNYAPYEGDESFLAGATEATTQLWDKVME
CCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKQENRTHAPVDFDTKMVSTITSHDAGYINKDLETIVGLQTDAPLKRAMLPNGGIRMVE
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEEEEE
GSCAAYNRELDADVKYIYSELRKTHNQGVFDVYTPEIMACRKSGVLTGLPDAYGRGRIIG
CCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGIDFLMKDKFAQFSSLQAQFEAGEDLSNVIQLREEIAEQHRALGQMKTMAAK
HHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDISRPAANAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIYIERDLKNGTITEQ
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCCCCCCCHH
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLNT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHH
LYTMGPSPEPNITVLWSDKLPVGFKKYCAKVSIDTSSIQYENDDLMRPDFQSDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKIQIAPKAAPITDEVLNFDDVMNRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCHHHHHHHH
DGLMDWLATQYVTALNSIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAQVKPVRDENGIAVDFEISGDYPKFGNNDPRVDDIACDLVERFMSKIRDRKMYRNAIP
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDEDGRRTNLAALMDGYFAHNEGHEGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHC
VNPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]