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The map label for this gene is trpCF [H]

Identifier: 117919965

GI number: 117919965

Start: 1767170

End: 1768651

Strand: Reverse

Name: trpCF [H]

Synonym: Shewana3_1517

Alternate gene names: 117919965

Gene position: 1768651-1767170 (Counterclockwise)

Preceding gene: 117919966

Following gene: 117919964

Centisome position: 35.57

GC content: 51.42

Gene sequence:

>1482_bases
ATGACACAGGTTCAACAACCCGACGCCCATCGCTCGGAAGATAATCAAGCATCGCAGGATGTAAGGGGTAGCATGCAAAC
CAGCACCGCAAAAGCCAACGTTTTAACCCGTATCGTCGATACTAAGGCCGCCCATATCGCCGCCCTTAAGTTGCGCTTTC
CTGAAGACAGCTTAACCCCCAAGATTTCGGATCGTAGTCTATTTGCTGCCTTAAAAGCCCCGAAGGCGGGTTATATTTTA
GAGTGTAAAAAAGCCAGTCCATCCAAAGGCTTAATCCGTAAAGACTTTGATGTCGAAGCCATCGCCTCGATTTATACCCA
ATATGCCGCTGGAATTTCAGTATTAACCGATGAGCAATTTTTCCAAGGGGACATTGACTATATCCCGAAAGTGCGGGCCA
AAGTGAGCCAACCGATTCTGTGTAAAGACTTTTTTGTTGACGAATATCAAGTCAAGTTGGCCGCTCACCAGGGCGCCGAT
GCGATTCTGCTGATGCTTTCTGTGCTCGAGGATGAGCGATATCAGCAACTTGCCAGCGAGGCGGCCAAGTATCAACTCGA
TGTGCTGACGGAAGTCAGCAATGAGGAAGAACTGAAACGCGCCATCGCCTTAAACGCACCTATTATTGGCATTAATAACC
GCAACTTACGGGATTTAAGTACCGATTTGGCCACCACAGAAGCCCTTGCGCCGCACATTGGCAGCGATCGCGTGGTGATC
AGCGAATCGGGCATTTACACTAACGAACAAGTGCGCCGCTTAAGTCCGTTAGTCGATGGCTTCTTAGTCGGTAGCTCGAT
TATGGCCGAAGAAGACATTGATTTAGCCTGCCGTAAATTGATCTTCGGTCACAATAAAGTCTGTGGCCTCACCCGCCTTG
AAGATATTCAAGCGGCGGCCACTGCCGGCGCCGTGTATGCAGGGCTGATTTTTGCTGAAAAATCGCCGCGCGCGCTCACC
CAAGAGGCCGCAAAGCAGTTAGTACAGCAATACCGCGCCGCCAACTTGCCCGCCATTGAGTTTGTCGGCGTATTTGTAAA
CAGCACACCTGAGCTGATGGCGAGTGTCGCCCAAGAATGCAGCTTAGCCGCGCTGCAACTGCATGGCAGTGAGACGGAAC
TGGAAATTGCCGAATTGAGCCAATTGCTGCAACAGGCCGGACTGAACACCCAGATTTGGAAGGCGGTCAGTGTCGATGCG
CAAAGCGGCGAGCTTGCTGCGATGCCAAGAGGTGTGCAGCGTTATCTTTTTGATAGCAAGAATGACGCAGGTTTTGGTGG
CACAGGGCAAGCCTTTAATTGGCAACTCCCCATTGCCCATAAAGCCGAGGCCATGCTCGCGGGCGGCCTCAATGCTGACA
ATGCTGCCCGTGCCAATGCCCAAGGATTTTACGGTTTAGATTTTAACTCTGGCCTCGAAACGGCTCCCGGCGTTAAGTCG
GCAGAGAAAATCCAAGCGGCATTTAGTCAACTACGACTCTAG

Upstream 100 bases:

>100_bases
CTGGCACCGCCCTTGCGCTCAATACCATAAAAAGCGGCAAAGCCTTTGAATTACTGAATCAACTCGCTAAAGTCAGCAGC
GAAGCACAGGAATAATGACC

Downstream 100 bases:

>100_bases
ACCAGCTTTTATAAAAACCTGTGCAAAGCACAGGCTAGCCAGAATTGAAACTCGATGGCGTTAACCTAAACCGCCATCGA
AACAAATGAATAGGATAAGG

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTQVQQPDAHRSEDNQASQDVRGSMQTSTAKANVLTRIVDTKAAHIAALKLRFPEDSLTPKISDRSLFAALKAPKAGYIL
ECKKASPSKGLIRKDFDVEAIASIYTQYAAGISVLTDEQFFQGDIDYIPKVRAKVSQPILCKDFFVDEYQVKLAAHQGAD
AILLMLSVLEDERYQQLASEAAKYQLDVLTEVSNEEELKRAIALNAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVI
SESGIYTNEQVRRLSPLVDGFLVGSSIMAEEDIDLACRKLIFGHNKVCGLTRLEDIQAAATAGAVYAGLIFAEKSPRALT
QEAAKQLVQQYRAANLPAIEFVGVFVNSTPELMASVAQECSLAALQLHGSETELEIAELSQLLQQAGLNTQIWKAVSVDA
QSGELAAMPRGVQRYLFDSKNDAGFGGTGQAFNWQLPIAHKAEAMLAGGLNADNAARANAQGFYGLDFNSGLETAPGVKS
AEKIQAAFSQLRL

Sequences:

>Translated_493_residues
MTQVQQPDAHRSEDNQASQDVRGSMQTSTAKANVLTRIVDTKAAHIAALKLRFPEDSLTPKISDRSLFAALKAPKAGYIL
ECKKASPSKGLIRKDFDVEAIASIYTQYAAGISVLTDEQFFQGDIDYIPKVRAKVSQPILCKDFFVDEYQVKLAAHQGAD
AILLMLSVLEDERYQQLASEAAKYQLDVLTEVSNEEELKRAIALNAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVI
SESGIYTNEQVRRLSPLVDGFLVGSSIMAEEDIDLACRKLIFGHNKVCGLTRLEDIQAAATAGAVYAGLIFAEKSPRALT
QEAAKQLVQQYRAANLPAIEFVGVFVNSTPELMASVAQECSLAALQLHGSETELEIAELSQLLQQAGLNTQIWKAVSVDA
QSGELAAMPRGVQRYLFDSKNDAGFGGTGQAFNWQLPIAHKAEAMLAGGLNADNAARANAQGFYGLDFNSGLETAPGVKS
AEKIQAAFSQLRL
>Mature_492_residues
TQVQQPDAHRSEDNQASQDVRGSMQTSTAKANVLTRIVDTKAAHIAALKLRFPEDSLTPKISDRSLFAALKAPKAGYILE
CKKASPSKGLIRKDFDVEAIASIYTQYAAGISVLTDEQFFQGDIDYIPKVRAKVSQPILCKDFFVDEYQVKLAAHQGADA
ILLMLSVLEDERYQQLASEAAKYQLDVLTEVSNEEELKRAIALNAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVIS
ESGIYTNEQVRRLSPLVDGFLVGSSIMAEEDIDLACRKLIFGHNKVCGLTRLEDIQAAATAGAVYAGLIFAEKSPRALTQ
EAAKQLVQQYRAANLPAIEFVGVFVNSTPELMASVAQECSLAALQLHGSETELEIAELSQLLQQAGLNTQIWKAVSVDAQ
SGELAAMPRGVQRYLFDSKNDAGFGGTGQAFNWQLPIAHKAEAMLAGGLNADNAARANAQGFYGLDFNSGLETAPGVKSA
EKIQAAFSQLRL

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=466, Percent_Identity=48.068669527897, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322638, Length=230, Percent_Identity=33.0434782608696, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 53347; Mature: 53216

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVQQPDAHRSEDNQASQDVRGSMQTSTAKANVLTRIVDTKAAHIAALKLRFPEDSLTP
CCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
KISDRSLFAALKAPKAGYILECKKASPSKGLIRKDFDVEAIASIYTQYAAGISVLTDEQF
CCCCHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHCCCEEEECCHH
FQGDIDYIPKVRAKVSQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLEDERYQQLASE
HCCCCHHCCHHHHHCCCCCEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAKYQLDVLTEVSNEEELKRAIALNAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVI
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SESGIYTNEQVRRLSPLVDGFLVGSSIMAEEDIDLACRKLIFGHNKVCGLTRLEDIQAAA
ECCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TAGAVYAGLIFAEKSPRALTQEAAKQLVQQYRAANLPAIEFVGVFVNSTPELMASVAQEC
HHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHH
SLAALQLHGSETELEIAELSQLLQQAGLNTQIWKAVSVDAQSGELAAMPRGVQRYLFDSK
CEEEEEECCCCCCEEHHHHHHHHHHCCCCHHHHHEEECCCCCCCEECCCHHHHHHHCCCC
NDAGFGGTGQAFNWQLPIAHKAEAMLAGGLNADNAARANAQGFYGLDFNSGLETAPGVKS
CCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCH
AEKIQAAFSQLRL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TQVQQPDAHRSEDNQASQDVRGSMQTSTAKANVLTRIVDTKAAHIAALKLRFPEDSLTP
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
KISDRSLFAALKAPKAGYILECKKASPSKGLIRKDFDVEAIASIYTQYAAGISVLTDEQF
CCCCHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHCCCEEEECCHH
FQGDIDYIPKVRAKVSQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLEDERYQQLASE
HCCCCHHCCHHHHHCCCCCEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AAKYQLDVLTEVSNEEELKRAIALNAPIIGINNRNLRDLSTDLATTEALAPHIGSDRVVI
HHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SESGIYTNEQVRRLSPLVDGFLVGSSIMAEEDIDLACRKLIFGHNKVCGLTRLEDIQAAA
ECCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TAGAVYAGLIFAEKSPRALTQEAAKQLVQQYRAANLPAIEFVGVFVNSTPELMASVAQEC
HHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHH
SLAALQLHGSETELEIAELSQLLQQAGLNTQIWKAVSVDAQSGELAAMPRGVQRYLFDSK
CEEEEEECCCCCCEEHHHHHHHHHHCCCCHHHHHEEECCCCCCCEECCCHHHHHHHCCCC
NDAGFGGTGQAFNWQLPIAHKAEAMLAGGLNADNAARANAQGFYGLDFNSGLETAPGVKS
CCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCH
AEKIQAAFSQLRL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]