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The map label for this gene is phrA [H]

Identifier: 117919615

GI number: 117919615

Start: 1376924

End: 1378405

Strand: Direct

Name: phrA [H]

Synonym: Shewana3_1166

Alternate gene names: 117919615

Gene position: 1376924-1378405 (Clockwise)

Preceding gene: 117919614

Following gene: 117919616

Centisome position: 27.69

GC content: 52.36

Gene sequence:

>1482_bases
ATGAGCGATAATCAAACCCCAGCGGCAAAGCACGCGTTGATGTGGTTTCGTCAGGATCTGCGTCTAACGGATAACCAAGC
GCTGACGGCCGCCTGTGATTGGGTGCGGCAAGCGCCTGGCGCAAAACTCAGGGCGATTTATATTGCGACTCCGAACCAGT
GGCTCAGCCACGATATGGCGCCGATCCAGATTGATTTTATCCAGCGGCATGTGAATTTACTTGCCCAAGGACTGGCAAGC
CTAGGGATTGAATTTGAGCTTATTCAGCTCGATAGCTTTAAAGAGGTGCCCGCTTTTTTAGCAAGTTACTGCGAAAAACT
TGGGGTTGAGCGGGTATTTGCGGGGCGAGAACCCGAAATCAACGAGCAAAAGCGCGACCAAGCCTGTCTCGATACGGGTA
TCCCCTTAGTGCTCAGCGACGAGCATTGCCTATTGCCGCCAGGTACTGTGCTTAACTTAAGCGGTGATATGTATAAGGTG
TTTACGCCCTTTAGCCGCAAGTGGCGAGAAATTGCCGCCAAACGCGCCATTTTTCCGCTTGCTGTGCCCGCGCCGCTTGG
CCCTATGTTACCTGAACCAAAGCCAATCAGTTTTAAAGAGCGTTTTCAATCGACAAGCAAGCTAACCTCGAGCGAGCAAT
GGGCGGCGGGAGAGGGGCAGGCAAAACGTATTCTTACCGCATTTATTAAGCAGAAAGTCCAAGACTATAAGCAGGATAGG
GACTTTCCTGCTCTCGATGGCACCAGTGTTATCTCCCCTTATTTGGCGCTAGGCGTGCTCAGCCCAAGGCAATGTATTGC
GGCGCTGCTTGGGCGTTTTCCCGATGTGATTGTCGATGATACTAGCCCTGGGCGAACCTGGTTGAATGAGCTTATCTGGC
GGGAGTTTTATCGTCATTTGTTGGTGGCTTTTCCTGATTTATCCAAGGGGCACAACTTTAATCGTCAGGCCGATCATGTG
CAGTGGCGTAACAACCAAGCTGAGTTTTTAGCGTGGTGCGAGGGCAAAACCGGTTATCCGATTGTGGATGCGGCGATGCG
CCAGCTCAATCAAACCGGCTGGATGCACAATCGGCTGCGCATGGTGGTGGCGAGCTTTTTAACCAAACACTTGCTTATCG
ATTGGCGCTGGGGTGAACGCTATTTCCGCCAGCACCTTATCGACGGCGATCTGGCGGCCAATAACGGTGGCTGGCAATGG
TCGGCGGGCTGTGGCTGTGATGCTCAGCCTTATTTCCGCATTTTTAATCCCATCAGCCAGAGCGAAAAGTTTGATCCAGA
TGGCAGCTTTATCCGTAAGTACTTACCAGAGCTGGCATCTTGGGGAATCAAGCGTTTGCACCAACCCAATATGCAAAAAA
CGCCTTCGTTATTTGAGGCGCCAATGCTGGAGTCTGACTCAGCTTATCCCTGTGCGATTGTGGAGCACAATGCGGCAAGG
GCGAGGGCGCTGGCGGTACTTGGGGTGCTGAAAAAGGCCTAG

Upstream 100 bases:

>100_bases
GCTGAATGGGTTAGCCAAGTTTCGGCGGCAAAAGAGCAACATAAGAAACAGCTGCGCAAGCAGAAAAACGCGGCTGAAGC
GCAGCAAGGAGAGCAAGCCG

Downstream 100 bases:

>100_bases
GCCATAGGCTCAACCTTACAACTTATCCCTTAGGGAGTTATCTGGATGATAAACCAAGCGAAGATTGAACAGAGCGGTGT
GGTTGATGAGTTTATCGCGC

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSDNQTPAAKHALMWFRQDLRLTDNQALTAACDWVRQAPGAKLRAIYIATPNQWLSHDMAPIQIDFIQRHVNLLAQGLAS
LGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDTGIPLVLSDEHCLLPPGTVLNLSGDMYKV
FTPFSRKWREIAAKRAIFPLAVPAPLGPMLPEPKPISFKERFQSTSKLTSSEQWAAGEGQAKRILTAFIKQKVQDYKQDR
DFPALDGTSVISPYLALGVLSPRQCIAALLGRFPDVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHV
QWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQW
SAGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEAPMLESDSAYPCAIVEHNAAR
ARALAVLGVLKKA

Sequences:

>Translated_493_residues
MSDNQTPAAKHALMWFRQDLRLTDNQALTAACDWVRQAPGAKLRAIYIATPNQWLSHDMAPIQIDFIQRHVNLLAQGLAS
LGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDTGIPLVLSDEHCLLPPGTVLNLSGDMYKV
FTPFSRKWREIAAKRAIFPLAVPAPLGPMLPEPKPISFKERFQSTSKLTSSEQWAAGEGQAKRILTAFIKQKVQDYKQDR
DFPALDGTSVISPYLALGVLSPRQCIAALLGRFPDVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHV
QWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQW
SAGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEAPMLESDSAYPCAIVEHNAAR
ARALAVLGVLKKA
>Mature_492_residues
SDNQTPAAKHALMWFRQDLRLTDNQALTAACDWVRQAPGAKLRAIYIATPNQWLSHDMAPIQIDFIQRHVNLLAQGLASL
GIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDTGIPLVLSDEHCLLPPGTVLNLSGDMYKVF
TPFSRKWREIAAKRAIFPLAVPAPLGPMLPEPKPISFKERFQSTSKLTSSEQWAAGEGQAKRILTAFIKQKVQDYKQDRD
FPALDGTSVISPYLALGVLSPRQCIAALLGRFPDVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHVQ
WRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWS
AGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEAPMLESDSAYPCAIVEHNAARA
RALAVLGVLKKA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=486, Percent_Identity=26.9547325102881, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4758072, Length=460, Percent_Identity=27.3913043478261, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI188536103, Length=390, Percent_Identity=27.6923076923077, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1786926, Length=490, Percent_Identity=45.9183673469388, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324962, Length=513, Percent_Identity=33.3333333333333, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17137248, Length=507, Percent_Identity=27.2189349112426, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24585455, Length=507, Percent_Identity=27.2189349112426, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24648152, Length=516, Percent_Identity=24.6124031007752, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55858; Mature: 55726

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQTPAAKHALMWFRQDLRLTDNQALTAACDWVRQAPGAKLRAIYIATPNQWLSHDMA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCC
PIQIDFIQRHVNLLAQGLASLGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEI
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NEQKRDQACLDTGIPLVLSDEHCLLPPGTVLNLSGDMYKVFTPFSRKWREIAAKRAIFPL
CHHHHHHHHHHCCCCEEECCCCEECCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCE
AVPAPLGPMLPEPKPISFKERFQSTSKLTSSEQWAAGEGQAKRILTAFIKQKVQDYKQDR
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFPALDGTSVISPYLALGVLSPRQCIAALLGRFPDVIVDDTSPGRTWLNELIWREFYRHL
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
LVAFPDLSKGHNFNRQADHVQWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLR
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHH
MVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPISQ
HHHHHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHCHHHC
SEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEAPMLESDSAYPCAIVEHNAAR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCHHH
ARALAVLGVLKKA
HHHHHHHHHHHCC
>Mature Secondary Structure 
SDNQTPAAKHALMWFRQDLRLTDNQALTAACDWVRQAPGAKLRAIYIATPNQWLSHDMA
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCC
PIQIDFIQRHVNLLAQGLASLGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEI
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NEQKRDQACLDTGIPLVLSDEHCLLPPGTVLNLSGDMYKVFTPFSRKWREIAAKRAIFPL
CHHHHHHHHHHCCCCEEECCCCEECCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCCE
AVPAPLGPMLPEPKPISFKERFQSTSKLTSSEQWAAGEGQAKRILTAFIKQKVQDYKQDR
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFPALDGTSVISPYLALGVLSPRQCIAALLGRFPDVIVDDTSPGRTWLNELIWREFYRHL
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
LVAFPDLSKGHNFNRQADHVQWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLR
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHH
MVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPISQ
HHHHHHHHHHHEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHCHHHC
SEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEAPMLESDSAYPCAIVEHNAAR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCHHH
ARALAVLGVLKKA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]