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The map label for this gene is rumA [H]

Identifier: 117919559

GI number: 117919559

Start: 1305325

End: 1306674

Strand: Direct

Name: rumA [H]

Synonym: Shewana3_1110

Alternate gene names: 117919559

Gene position: 1305325-1306674 (Clockwise)

Preceding gene: 117919555

Following gene: 117919560

Centisome position: 26.25

GC content: 50.0

Gene sequence:

>1350_bases
ATGGCACAATTTTTTAAAGCAAAACCAAATAGTTCCAAGCAATTGTCCGCGAAGCAATCCTTTAGCGTGCACCAGCTGGA
TCATCTAGGGGCAGGTATAGCACAACATCAGGGAAAAGTCGTATTTATCCCCGGCGCCTTGCCGAATGAAACCGTGCAGG
CGCAGCTGACCGAACAAAAGAAAAACTATGCCCGAGCCAAGCTGATTAAAGTTGAGACACCGAGCGCTGAGCGAGTCACG
CCGCTATGCCCACATTACCAAAGCTGTGGTGGCTGCGATTTACAGCATATGTCATTAGCAGGGCAGCGCGAGCATAAGTC
CGCGGCCTTGGTTGATATTATGGCCAAATTTGCTGGTGCCGAAGGCAACTCAGTGCCTGCATTGACGGGCGAAGGTTGGC
ACTATCGTCGCCGCGCCCGCCTTGCGACCCTATTTGATAAAAATACCAAGCAGTTGAGCCTAGGGTTTCGCGCTTCGAGC
AGCAACCAAGTGGTGCCTATTGATAGCTGTTTAGTCTTGGCGAAACCCTTAGCGGATTTAATTGCCCCATTTGCTAAGTT
GCTTAATCAACTGGCGGCCAAGTCGAGCCTTGGGCATGTAGAATTAATTGATGCCGACAACGGCCATTTTGCGGTAATTC
GGATCACTAAACCCTTAAATGATAAGGATATGGCTAAGCTGGCGCAGTTTGCTGAGCAGCATCAAATCCATATTTGTCTG
CAAGACAACAACGGCGAGTTCCATGGAGTGAATGGCACGCTGTTATTGCCCGTTTACCAGTTACTCGATGATAACGCCGA
TGCAACGCCTGTGAGCCTCACCTTTACCCCTGGTAACTTTGTGCAAGTGAATGCGCAAATTAACAAGACCATGGTGGCGC
AGGCGTTAGAATGGTTAGCACCACAGCCGGGTGAGCGAATTCTCGATCTCTTCTGCGGTATGGGGAACTTCAGTTTGCCT
TTAGCTAAACTGGGCGCCGAAGTGATTGGGGTTGAAGGGGTGCCAGAAATGGTCAGCCAAGCCCGCGAAAACGCGGCGGC
AAATGGCTTAAGCAATTTAACCTTTTACCATGGCGACTTAAGCGCCGATTTATCCTGTGAGCCTTGGATGGGGAAGATTG
ATAAGTTGCTACTCGATCCTGCCCGCGCGGGTGCGTTCGAGAGTTTGCAATGGCTTAAGAAGATGAAACCCCGCCAAGTG
GTTTATGTCTCTTGTAATCCGGCGAGCTTGGCCCGCGACAGTGCTGTGTTGCTCGAGCGTGGTTACAAGTTACAAAAGTT
AGGTTTAATCGATATGTTCCCGCAGACACATCATATCGAAGCCATGGCCTTGTTTGAGCTTGCAAAATAA

Upstream 100 bases:

>100_bases
GCAGCAGCGCAGTTTCCTAAATTCTAGGGACGATATAATTTTATAAGACTGCCAGAAAAAGGGCGGTCTTTACCAGTTTT
TTATTGATTTAGAGGCCGAA

Downstream 100 bases:

>100_bases
GGCTTATCGATTGGGCAAGGTTTCCTCACTTGCCCGCGTTATTCGAGTGTAAAGTAAGCAAAGCGTTTGAATAACGTAAA
AAATGTGAAGTTTCGTCTCG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPNETVQAQLTEQKKNYARAKLIKVETPSAERVT
PLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASS
SNQVVPIDSCLVLAKPLADLIAPFAKLLNQLAAKSSLGHVELIDADNGHFAVIRITKPLNDKDMAKLAQFAEQHQIHICL
QDNNGEFHGVNGTLLLPVYQLLDDNADATPVSLTFTPGNFVQVNAQINKTMVAQALEWLAPQPGERILDLFCGMGNFSLP
LAKLGAEVIGVEGVPEMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQV
VYVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK

Sequences:

>Translated_449_residues
MAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPNETVQAQLTEQKKNYARAKLIKVETPSAERVT
PLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASS
SNQVVPIDSCLVLAKPLADLIAPFAKLLNQLAAKSSLGHVELIDADNGHFAVIRITKPLNDKDMAKLAQFAEQHQIHICL
QDNNGEFHGVNGTLLLPVYQLLDDNADATPVSLTFTPGNFVQVNAQINKTMVAQALEWLAPQPGERILDLFCGMGNFSLP
LAKLGAEVIGVEGVPEMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQV
VYVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK
>Mature_448_residues
AQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPNETVQAQLTEQKKNYARAKLIKVETPSAERVTP
LCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGAEGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASSS
NQVVPIDSCLVLAKPLADLIAPFAKLLNQLAAKSSLGHVELIDADNGHFAVIRITKPLNDKDMAKLAQFAEQHQIHICLQ
DNNGEFHGVNGTLLLPVYQLLDDNADATPVSLTFTPGNFVQVNAQINKTMVAQALEWLAPQPGERILDLFCGMGNFSLPL
AKLGAEVIGVEGVPEMVSQARENAAANGLSNLTFYHGDLSADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQVV
YVSCNPASLARDSAVLLERGYKLQKLGLIDMFPQTHHIEAMALFELAK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=199, Percent_Identity=34.6733668341709, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI51173878, Length=199, Percent_Identity=34.6733668341709, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1789148, Length=452, Percent_Identity=45.1327433628319, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787083, Length=177, Percent_Identity=32.7683615819209, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=32.8282828282828, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 48958; Mature: 48827

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPNETVQAQLTEQK
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHCCCEEEECCCCCCCHHHHHHHHHH
KNYARAKLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGA
HHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCC
EGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASSSNQVVPIDSCLVLAKPLADL
CCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHH
IAPFAKLLNQLAAKSSLGHVELIDADNGHFAVIRITKPLNDKDMAKLAQFAEQHQIHICL
HHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEE
QDNNGEFHGVNGTLLLPVYQLLDDNADATPVSLTFTPGNFVQVNAQINKTMVAQALEWLA
ECCCCCEECCCCEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHC
PQPGERILDLFCGMGNFSLPLAKLGAEVIGVEGVPEMVSQARENAAANGLSNLTFYHGDL
CCCCHHHHHHHHCCCCCCCCHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
SADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQVVYVSCNPASLARDSAVLLER
CCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHC
GYKLQKLGLIDMFPQTHHIEAMALFELAK
CCCEEECCHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AQFFKAKPNSSKQLSAKQSFSVHQLDHLGAGIAQHQGKVVFIPGALPNETVQAQLTEQK
CCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHCCCEEEECCCCCCCHHHHHHHHHH
KNYARAKLIKVETPSAERVTPLCPHYQSCGGCDLQHMSLAGQREHKSAALVDIMAKFAGA
HHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCC
EGNSVPALTGEGWHYRRRARLATLFDKNTKQLSLGFRASSSNQVVPIDSCLVLAKPLADL
CCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHH
IAPFAKLLNQLAAKSSLGHVELIDADNGHFAVIRITKPLNDKDMAKLAQFAEQHQIHICL
HHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEE
QDNNGEFHGVNGTLLLPVYQLLDDNADATPVSLTFTPGNFVQVNAQINKTMVAQALEWLA
ECCCCCEECCCCEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHC
PQPGERILDLFCGMGNFSLPLAKLGAEVIGVEGVPEMVSQARENAAANGLSNLTFYHGDL
CCCCHHHHHHHHCCCCCCCCHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
SADLSCEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRQVVYVSCNPASLARDSAVLLER
CCCCCCCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHC
GYKLQKLGLIDMFPQTHHIEAMALFELAK
CCCEEECCHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]