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The map label for this gene is bepF [H]

Identifier: 117919526

GI number: 117919526

Start: 1262443

End: 1263681

Strand: Direct

Name: bepF [H]

Synonym: Shewana3_1077

Alternate gene names: 117919526

Gene position: 1262443-1263681 (Clockwise)

Preceding gene: 117919524

Following gene: 117919527

Centisome position: 25.39

GC content: 51.9

Gene sequence:

>1239_bases
ATGAAAAGTAACCAACCTTTACGGATATTGATGTTGACTGCCGTGGCGGCTTTTGTCCTTTCGGCCTGTGGTGAACCCGA
AGCCCAGCAGGGGCAAGCACCGGGTGCACCTAAGGTCGATGTTGCGCAGGTATTGCATGAGCGCGTTACCGAGTGGGATG
AATTTACCGGCCGTTTGCAGGCGCCAGAGAGTGTGACCTTAGTGCCACGCGTATCTGGCTATATCGCGTCGGTAAACTTT
AAAGAAGGCGCCTTAGTGAAAAAAGGCGATGTGCTATTCCATATCGATGCGAGTGTGTTCGAAGCGGAAGTGGCCCGGTT
AAGAGCAGATCTTGCTAGCGCGCTCTCTGCTGAGCAACTGGCCACTAACGACTTAGAACGTGCCCGTAAACTCTTTGCCC
AAAAGGCGGTATCTGCAGAATTACTCGATACCCGTGAGTCTAACAAACGTCAAACCACGGCGGCCGTAGCCTCTGTAAAA
GCTGCGCTGCTGCGCGCTGAGTTGGACTTGGATTACACCCAAGTGCGCGCGCCGATTGATGGCCGAGCCTCCTACGCCAA
TGTCACTGCGGGTAACTATGTGTCTGCGGGGCAGAGTGTGTTAACCAGCTTAGTATCGACCGCCAGCATGTATGCCTACT
TCGATGTGGATGAGCAAACCTATCTGAAATACGTCAAGTTAACCGCCGAGAAAAAGCGTAAAGACCCTCGTGCCGGCGAC
AATCCCGTGTATATGGCGCTGGCTAACGAGCGCGAATATCAACACATAGGCCTAGTGGACTTTGTCGATAACTCGATGGA
TAGACAGACGGGCACTATTCGAGTACGCGCCACGTTTAGCAATGAAGACAACAGCTTGCTCCCAGGTCTGTTCGCCAGAT
TACGTACCGCCGGTAGCGGCGCCTATGACGGCATCTTAATTGACGATAAAGCCGTGGGCACGGATCTGAACAACAAGTTC
GTGTTAGTGGTGGCAAACGATGGCACTGTTGAGTATCGCGGCGTCACCCTAGGTGAAAAAGTTCAAGGTTTACGCATTGT
TAAGCAAGGTTTAGCGGCAGACGACAAAATCGTCGTCAATGGCATGCAAAGGGTTCGTCCTAAGATGCAAATTGAGCCAC
ACCTGGTTGATATGGCCGACAGCGAACAGCTAGAGGCGCTTCGTCAAGCTCAATTACTGCTTGATAAACATCAAGAAAAT
ATCACCACTCCAGACGTTGAAGTAGCGAGCCGCGGTTAA

Upstream 100 bases:

>100_bases
TAATATTTTTTACCAGTCGGTATTTATTGGTTTTGACCGAGTGTTCATTACTGATGTCTGTCTTAAACGAGATAAATGCC
TGCCACATGAGGATTGTAAG

Downstream 100 bases:

>100_bases
GGAATCCAACAGAATGTTGTCGCAGTTTTTTATTAAAAGACCGATATTTGCAGCCGTTCTCTCGCTGCTGTTTTTTATCA
CAGGGGCGATTGCCGTCTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKSNQPLRILMLTAVAAFVLSACGEPEAQQGQAPGAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLVPRVSGYIASVNF
KEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAEQLATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVK
AALLRAELDLDYTQVRAPIDGRASYANVTAGNYVSAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRKDPRAGD
NPVYMALANEREYQHIGLVDFVDNSMDRQTGTIRVRATFSNEDNSLLPGLFARLRTAGSGAYDGILIDDKAVGTDLNNKF
VLVVANDGTVEYRGVTLGEKVQGLRIVKQGLAADDKIVVNGMQRVRPKMQIEPHLVDMADSEQLEALRQAQLLLDKHQEN
ITTPDVEVASRG

Sequences:

>Translated_412_residues
MKSNQPLRILMLTAVAAFVLSACGEPEAQQGQAPGAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLVPRVSGYIASVNF
KEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAEQLATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVK
AALLRAELDLDYTQVRAPIDGRASYANVTAGNYVSAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRKDPRAGD
NPVYMALANEREYQHIGLVDFVDNSMDRQTGTIRVRATFSNEDNSLLPGLFARLRTAGSGAYDGILIDDKAVGTDLNNKF
VLVVANDGTVEYRGVTLGEKVQGLRIVKQGLAADDKIVVNGMQRVRPKMQIEPHLVDMADSEQLEALRQAQLLLDKHQEN
ITTPDVEVASRG
>Mature_412_residues
MKSNQPLRILMLTAVAAFVLSACGEPEAQQGQAPGAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLVPRVSGYIASVNF
KEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAEQLATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVK
AALLRAELDLDYTQVRAPIDGRASYANVTAGNYVSAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRKDPRAGD
NPVYMALANEREYQHIGLVDFVDNSMDRQTGTIRVRATFSNEDNSLLPGLFARLRTAGSGAYDGILIDDKAVGTDLNNKF
VLVVANDGTVEYRGVTLGEKVQGLRIVKQGLAADDKIVVNGMQRVRPKMQIEPHLVDMADSEQLEALRQAQLLLDKHQEN
ITTPDVEVASRG

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=382, Percent_Identity=31.6753926701571, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789929, Length=371, Percent_Identity=29.1105121293801, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=29.9465240641711, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082036, Length=343, Percent_Identity=27.4052478134111, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081951, Length=172, Percent_Identity=35.4651162790698, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789637, Length=164, Percent_Identity=33.5365853658537, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44968; Mature: 44968

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00850 GLY_RADICAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNQPLRILMLTAVAAFVLSACGEPEAQQGQAPGAPKVDVAQVLHERVTEWDEFTGRLQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
APESVTLVPRVSGYIASVNFKEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAEQL
CCCCEEEEEECCCEEEEEECCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
ATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVKAALLRAELDLDYTQVRAPID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEECCCC
GRASYANVTAGNYVSAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRKDPRAGD
CCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC
NPVYMALANEREYQHIGLVDFVDNSMDRQTGTIRVRATFSNEDNSLLPGLFARLRTAGSG
CCEEEEEECCCCCCEECHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCC
AYDGILIDDKAVGTDLNNKFVLVVANDGTVEYRGVTLGEKVQGLRIVKQGLAADDKIVVN
CCCCEEEECCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHEEC
GMQRVRPKMQIEPHLVDMADSEQLEALRQAQLLLDKHQENITTPDVEVASRG
CHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSNQPLRILMLTAVAAFVLSACGEPEAQQGQAPGAPKVDVAQVLHERVTEWDEFTGRLQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
APESVTLVPRVSGYIASVNFKEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAEQL
CCCCEEEEEECCCEEEEEECCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
ATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVKAALLRAELDLDYTQVRAPID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEECCCC
GRASYANVTAGNYVSAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRKDPRAGD
CCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC
NPVYMALANEREYQHIGLVDFVDNSMDRQTGTIRVRATFSNEDNSLLPGLFARLRTAGSG
CCEEEEEECCCCCCEECHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCC
AYDGILIDDKAVGTDLNNKFVLVVANDGTVEYRGVTLGEKVQGLRIVKQGLAADDKIVVN
CCCCEEEECCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHEEC
GMQRVRPKMQIEPHLVDMADSEQLEALRQAQLLLDKHQENITTPDVEVASRG
CHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]