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The map label for this gene is paaF [H]

Identifier: 117919173

GI number: 117919173

Start: 838482

End: 839219

Strand: Reverse

Name: paaF [H]

Synonym: Shewana3_0721

Alternate gene names: 117919173

Gene position: 839219-838482 (Counterclockwise)

Preceding gene: 117919177

Following gene: 117919167

Centisome position: 16.88

GC content: 46.48

Gene sequence:

>738_bases
ATGAGTCATATACAGGTTCGGGATGATCAGGGCGTTCGTATTATCAGCTTTAATCGCCCAGATAAACGCAATGCACTTGA
TCTTAATATGTATAAACAACTGACAGAATACCTGATCGAAGGTGAAGCAGACAACGACATTCGTGCCTTTATGCTCCATG
GCGAAGATAATTGTTTCACCTCGGGCAATGATGTTGCTGACTTTTTGAAAAATAGTGACTTAGGTCCAAATCATCCTGCG
GTGCGCTTCCTATTTTGCCTATTGGAACTCAAAAAACCACTGGTCGCCGCCGTCTCTGGTGCCGCCGTTGGCATAGGCAC
AACGGTACTGCTTCACTGTGATTTGGTCTATGCCGACAACACGGCTAAATTCCAACTGCCCTTCGTGAATTTGGCCCTAG
TGCCCGAGGCTGGCGCAAGCTTACTCTTACCCGAATTGGTGGGTTATCAAAAGGCGGCGGAGCTCTTGCTGCTCGGCGAA
AGTTTTGATGCAAATACAGCACACAGGCTGAATATCATTAACGATGTAATAGCCCAAGAAGAGTTACTCGGTTACGCCCT
GAGTCAGGCCAAGAAACTGGCGAATCAGCCACCGCAAGCACTGCAAATCACCCGTCAGTTGATGCGACCACATAAAAACC
GCGTGCAACACCAGATGCATCAAGAATTAGAACAATTTAGTGCAAGACTTAAAAGCGATGAAGCAAAAGCCAGATTTCAG
GCTTTTCTTAAAAAGTGA

Upstream 100 bases:

>100_bases
AGCACAGGATAATGCGACAAAATTAAACATCTGTTTGAATATCGGTTTCAATGTCTTAAACTCCATGTCTACCCAATGTC
GTCGTAAAGGAAAACAACCC

Downstream 100 bases:

>100_bases
TGCTCACTTAAACAGTGTTCACTATTGGCATGAGGGTCGATGCGCCCTCTTGTTGATAGCATCCCGCTAAAGGCTGTGTC
CATAAACTTGGATCGGCAAT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSHIQVRDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGEDNCFTSGNDVADFLKNSDLGPNHPA
VRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGE
SFDANTAHRLNIINDVIAQEELLGYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKARFQ
AFLKK

Sequences:

>Translated_245_residues
MSHIQVRDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGEDNCFTSGNDVADFLKNSDLGPNHPA
VRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGE
SFDANTAHRLNIINDVIAQEELLGYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKARFQ
AFLKK
>Mature_244_residues
SHIQVRDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGEDNCFTSGNDVADFLKNSDLGPNHPAV
RFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADNTAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGES
FDANTAHRLNIINDVIAQEELLGYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKARFQA
FLKK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=30.6772908366534, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=30.6772908366534, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=30.6772908366534, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI25453481, Length=230, Percent_Identity=26.5217391304348, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI51538573, Length=230, Percent_Identity=26.5217391304348, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI4757966, Length=230, Percent_Identity=26.5217391304348, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI51538581, Length=230, Percent_Identity=26.5217391304348, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=27.0161290322581, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4757968, Length=230, Percent_Identity=25.6521739130435, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI49355787, Length=230, Percent_Identity=25.6521739130435, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI221136756, Length=234, Percent_Identity=26.0683760683761, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI221136753, Length=234, Percent_Identity=26.0683760683761, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI221307494, Length=234, Percent_Identity=26.0683760683761, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI116235487, Length=214, Percent_Identity=25.2336448598131, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI31542718, Length=195, Percent_Identity=27.1794871794872, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI70995211, Length=207, Percent_Identity=28.0193236714976, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=28.9473684210526, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=210, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502327, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI213417737, Length=212, Percent_Identity=23.5849056603774, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=212, Percent_Identity=23.5849056603774, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=32.6612903225806, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=34.3629343629344, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI221142681, Length=241, Percent_Identity=28.2157676348548, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788597, Length=201, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=27.4725274725275, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=28.9256198347107, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=26.9387755102041, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=239, Percent_Identity=27.1966527196653, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=32.1951219512195, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=202, Percent_Identity=31.1881188118812, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=25.296442687747, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=206, Percent_Identity=25.2427184466019, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=27.9329608938547, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=227, Percent_Identity=23.3480176211454, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=30.188679245283, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=28.9256198347107, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=28.9256198347107, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=220, Percent_Identity=30.4545454545455, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24653139, Length=195, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357171, Length=218, Percent_Identity=27.5229357798165, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=27.1428571428571, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27374; Mature: 27243

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIQVRDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGEDNCFT
CCCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SGNDVADFLKNSDLGPNHPAVRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADN
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEEEEECC
TAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGESFDANTAHRLNIINDVIAQE
CCEEEECEEEEEECCCCCCHHHHHHHHCHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHH
ELLGYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKARFQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKK
HHHCC
>Mature Secondary Structure 
SHIQVRDDQGVRIISFNRPDKRNALDLNMYKQLTEYLIEGEADNDIRAFMLHGEDNCFT
CCEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SGNDVADFLKNSDLGPNHPAVRFLFCLLELKKPLVAAVSGAAVGIGTTVLLHCDLVYADN
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEEEEECC
TAKFQLPFVNLALVPEAGASLLLPELVGYQKAAELLLLGESFDANTAHRLNIINDVIAQE
CCEEEECEEEEEECCCCCCHHHHHHHHCHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHH
ELLGYALSQAKKLANQPPQALQITRQLMRPHKNRVQHQMHQELEQFSARLKSDEAKARFQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]