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The map label for this gene is hydA [H]

Identifier: 117919163

GI number: 117919163

Start: 825361

End: 826593

Strand: Reverse

Name: hydA [H]

Synonym: Shewana3_0711

Alternate gene names: 117919163

Gene position: 826593-825361 (Counterclockwise)

Preceding gene: 117919164

Following gene: 117919162

Centisome position: 16.62

GC content: 50.45

Gene sequence:

>1233_bases
ATGACAGCGACAACTTATCAACCGGGAGAAATCCAAGGGCTGATCAAGATAAATGCATCTAAATGTAAAGGATGTGATGC
TTGTAAACAGTTCTGCCCAACCCATGCCATTCATGGCGCCTCGGGTGCAGTGCACTCTATTGATGAAGATAAATGCTTAA
GCTGCGGCCAATGTTTAATTAACTGCCCCTTTAGCGCCATTGAAGAAACCCACAGCGCCCTTGAAACCGTCATTAAAAAG
CTCGCCGATAAAAATACTACCGTCGTCGGCATTATCGCCCCTGCCGTGCGGGTAGCAATTGGTGAAGAATTCGGGTTAGG
CACGGGCGAACTCGTGACGGGTAAGCTCTATGGCGCGATGAATCAGGCAGGCTTTAAGATTTTCGACTGTAACTTTGCCG
CCGATCTCACCATCATGGAAGAAGGCAGTGAGTTTATTCATCGCCTCCATGCCAATGTCAAAGGTGAAGAAAATGCGGGT
GCGCTACCGCAGTTCACCTCCTGCTGCCCTGGTTGGGTGCGCTACCTCGAAACCCGCTATCCAGCGCTACTGCCTAACCT
ATCCACCGCTAAATCCCCTCAGCAAATGGCAGGAACGGTCGCCAAAACCTATGGCGCTAAGGTGTATCAAATGCAGCCAG
AGAATATTTTCACTGTCTCTGTGATGCCTTGTACCTCGAAAAAACTCGAAGCCTCACGCCCCGAATTTAACTCGGCTTGG
CAATACCATCAGGAACAAGGTGCTAACACGCCCTCGTACCAAGATATTGATGCCGTGCTGACCACCCGAGAAATGGCTCA
ATTGCTCAAAATGCTCGATATCGACCTCGCTAATACCCCCGAATATCAAGGCGATAGCATGTTCTCTGAATACACAGGCG
CGGGCACTATCTTTGGTACCACAGGGGGTGTGATGGAAGCGGCATTACGTACCGCCCATAAAGTGCTCACAGGCACTGAA
ATGGCCAAGTTGGAATTTGAACCCGTACGCGGGCTAAAAGGCGTCAAATCGGCTTCCGTCAGCCTGTTTGATACTCAGCT
CAACCAGAATGTAACGGTCAATGTCGCAGTGGTACACGACATGGGCAACAACATCGAACCCGTGCTGCGCGACGTAATGG
CTGGCACCTCGCCTTATCACTTTATCGAAGTGATGAACTGCGCTGGCGGTTGCGTCAACGGTGGAGGCCAACCTATTGAA
GGTAAAGGCTCTTCTTGGCTAGGTAACATTTAA

Upstream 100 bases:

>100_bases
CTTACCAATACGCTTTTGCCCGAGGCATAAGCTGCTAAATAAATACATTAAATAACACTGAAAATATAATTCAGGTTGAA
AATGAGGAACGCGAAATCCT

Downstream 100 bases:

>100_bases
CGGGAAACAGATATGAACAAGAAAAAACACCTATTTGCTGAGGACAGTTTCTTTCTGTCACGCCGTAAGTTTATGGCCGT
AGGCGCTGCCTTTGTCGCCG

Product: response regulator receiver protein

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTATTYQPGEIQGLIKINASKCKGCDACKQFCPTHAIHGASGAVHSIDEDKCLSCGQCLINCPFSAIEETHSALETVIKK
LADKNTTVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNQAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEENAG
ALPQFTSCCPGWVRYLETRYPALLPNLSTAKSPQQMAGTVAKTYGAKVYQMQPENIFTVSVMPCTSKKLEASRPEFNSAW
QYHQEQGANTPSYQDIDAVLTTREMAQLLKMLDIDLANTPEYQGDSMFSEYTGAGTIFGTTGGVMEAALRTAHKVLTGTE
MAKLEFEPVRGLKGVKSASVSLFDTQLNQNVTVNVAVVHDMGNNIEPVLRDVMAGTSPYHFIEVMNCAGGCVNGGGQPIE
GKGSSWLGNI

Sequences:

>Translated_410_residues
MTATTYQPGEIQGLIKINASKCKGCDACKQFCPTHAIHGASGAVHSIDEDKCLSCGQCLINCPFSAIEETHSALETVIKK
LADKNTTVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNQAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEENAG
ALPQFTSCCPGWVRYLETRYPALLPNLSTAKSPQQMAGTVAKTYGAKVYQMQPENIFTVSVMPCTSKKLEASRPEFNSAW
QYHQEQGANTPSYQDIDAVLTTREMAQLLKMLDIDLANTPEYQGDSMFSEYTGAGTIFGTTGGVMEAALRTAHKVLTGTE
MAKLEFEPVRGLKGVKSASVSLFDTQLNQNVTVNVAVVHDMGNNIEPVLRDVMAGTSPYHFIEVMNCAGGCVNGGGQPIE
GKGSSWLGNI
>Mature_409_residues
TATTYQPGEIQGLIKINASKCKGCDACKQFCPTHAIHGASGAVHSIDEDKCLSCGQCLINCPFSAIEETHSALETVIKKL
ADKNTTVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAMNQAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEENAGA
LPQFTSCCPGWVRYLETRYPALLPNLSTAKSPQQMAGTVAKTYGAKVYQMQPENIFTVSVMPCTSKKLEASRPEFNSAWQ
YHQEQGANTPSYQDIDAVLTTREMAQLLKMLDIDLANTPEYQGDSMFSEYTGAGTIFGTTGGVMEAALRTAHKVLTGTEM
AKLEFEPVRGLKGVKSASVSLFDTQLNQNVTVNVAVVHDMGNNIEPVLRDVMAGTSPYHFIEVMNCAGGCVNGGGQPIEG
KGSSWLGNI

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=358, Percent_Identity=31.0055865921788, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI6912524, Length=374, Percent_Identity=29.144385026738, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI134284357, Length=319, Percent_Identity=31.0344827586207, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI84452151, Length=319, Percent_Identity=31.0344827586207, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI14165461, Length=411, Percent_Identity=28.2238442822384, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71995015, Length=356, Percent_Identity=25.8426966292135, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324089, Length=394, Percent_Identity=24.1116751269036, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI116007464, Length=370, Percent_Identity=29.1891891891892, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI116007470, Length=370, Percent_Identity=29.1891891891892, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI116007466, Length=370, Percent_Identity=29.1891891891892, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI116007468, Length=370, Percent_Identity=29.1891891891892, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI116007462, Length=245, Percent_Identity=32.6530612244898, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 43988; Mature: 43856

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATTYQPGEIQGLIKINASKCKGCDACKQFCPTHAIHGASGAVHSIDEDKCLSCGQCLI
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
NCPFSAIEETHSALETVIKKLADKNTTVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAM
HCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCEEEHHHHHHH
NQAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEENAGALPQFTSCCPGWVRYLETRY
CCCCCEEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHCC
PALLPNLSTAKSPQQMAGTVAKTYGAKVYQMQPENIFTVSVMPCTSKKLEASRPEFNSAW
CHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCHHCCCCCCHHHHH
QYHQEQGANTPSYQDIDAVLTTREMAQLLKMLDIDLANTPEYQGDSMFSEYTGAGTIFGT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEC
TGGVMEAALRTAHKVLTGTEMAKLEFEPVRGLKGVKSASVSLFDTQLNQNVTVNVAVVHD
CHHHHHHHHHHHHHHHCCCHHHEEECCHHCCCCCCCCCCEEEEEHHCCCCEEEEEEEEEC
MGNNIEPVLRDVMAGTSPYHFIEVMNCAGGCVNGGGQPIEGKGSSWLGNI
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATTYQPGEIQGLIKINASKCKGCDACKQFCPTHAIHGASGAVHSIDEDKCLSCGQCLI
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
NCPFSAIEETHSALETVIKKLADKNTTVVGIIAPAVRVAIGEEFGLGTGELVTGKLYGAM
HCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCEEEHHHHHHH
NQAGFKIFDCNFAADLTIMEEGSEFIHRLHANVKGEENAGALPQFTSCCPGWVRYLETRY
CCCCCEEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHCC
PALLPNLSTAKSPQQMAGTVAKTYGAKVYQMQPENIFTVSVMPCTSKKLEASRPEFNSAW
CHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCHHCCCCCCHHHHH
QYHQEQGANTPSYQDIDAVLTTREMAQLLKMLDIDLANTPEYQGDSMFSEYTGAGTIFGT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEC
TGGVMEAALRTAHKVLTGTEMAKLEFEPVRGLKGVKSASVSLFDTQLNQNVTVNVAVVHD
CHHHHHHHHHHHHHHHCCCHHHEEECCHHCCCCCCCCCCEEEEEHHCCCCEEEEEEEEEC
MGNNIEPVLRDVMAGTSPYHFIEVMNCAGGCVNGGGQPIEGKGSSWLGNI
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3888621; 3322275; 11456963; 10368269; 11456758 [H]