Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 117919122

GI number: 117919122

Start: 791202

End: 791645

Strand: Reverse

Name: ptsN [H]

Synonym: Shewana3_0670

Alternate gene names: 117919122

Gene position: 791645-791202 (Counterclockwise)

Preceding gene: 117919123

Following gene: 117919121

Centisome position: 15.92

GC content: 48.87

Gene sequence:

>444_bases
ATGGAACTTCGTACCATACTGCGGCCGGAATGCACGACCTGCGCCACTCCGGGCAGTAAGAAAAAGGTACTGGAACTTAT
CAGCGACTTAGCCGCTGCCCAGTACCCCACCCTCTCATCTCAAGAGATCTTCGAAAGCCTAGTGGCGCGCGAAAAGATGG
GGAGCACAGGTATAGGTAACGGCATCGCCATTCCACATGGTCGATTAACCGATATAACACAACCCATAGCCATATTGGTG
AAATGTGAGGAGCCGATAGCCTTTGATGCTATCGATAAACAACCTGTCGACATTTTATTTGCGCTGCTGGTGCCTGCTGA
CCAATGTCAACAGCACTTAAGCACTTTGTCCTGCATGGCAGAAAAGCTCAGCGACAAACAAATATTGAAGCAATTGCGCA
AGACGCATGATGAAACGGAACTCTATCAGGTAATCACTGGATGA

Upstream 100 bases:

>100_bases
CTTCAGAGCATGCAGATATGTATGCTGCCATAGACGTCCTGATCGACAAGCTTGATCGTCAGGTTATTAAACACAAAGAG
AAGTTAACAAAACACTAGCA

Downstream 100 bases:

>100_bases
AACTGGTCATAGTGTCAGGGCGCTCAGGCTCGGGCAAATCGGTCGCACTAAGAGTGCTTGAAGATCTCGGCTACTATTGT
GTCGATAATCTTCCTTTGCC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLTDITQPIAILV
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG

Sequences:

>Translated_147_residues
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLTDITQPIAILV
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG
>Mature_147_residues
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGNGIAIPHGRLTDITQPIAILV
KCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMAEKLSDKQILKQLRKTHDETELYQVITG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=55.4054054054054, Blast_Score=159, Evalue=6e-41,
Organism=Escherichia coli, GI2367327, Length=112, Percent_Identity=27.6785714285714, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1786951, Length=129, Percent_Identity=22.4806201550388, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16148; Mature: 16148

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGN
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLTDITQPIAILVKCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMA
CCCCCCCCHHHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLSDKQILKQLRKTHDETELYQVITG
HHHCHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGN
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLTDITQPIAILVKCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMA
CCCCCCCCHHHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLSDKQILKQLRKTHDETELYQVITG
HHHCHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]