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The map label for this gene is nrfC [H]

Identifier: 117919087

GI number: 117919087

Start: 748128

End: 748883

Strand: Reverse

Name: nrfC [H]

Synonym: Shewana3_0635

Alternate gene names: 117919087

Gene position: 748883-748128 (Counterclockwise)

Preceding gene: 117919095

Following gene: 117919086

Centisome position: 15.06

GC content: 54.1

Gene sequence:

>756_bases
ATGGACAAGAGTAAACGTCAGTTTCTCGGAAAAATGGCCAGTGTGAGCGTCGGCGCCGCGCTCATTCCCGTCGTGCAAGT
GCAAGCCCAACCGGCTCAAACTGCAACTACGAGTAAACGCTACGGCATGGTGATTGATCTGCGCCGCTGCGTCGGCTGTC
AGGCCTGTACCGTGGCGTGCACCTTCGAAAACCTGCCACCCTTGGGGCAATTTCGTACCACAGTGCAGCAATACGAAGTG
TCGCAACAGGATTCTGCTACGCCGCCCGCATTTTTGATGTTGCCAAGACTGTGTAATCACTGTGAAAACCCGCCTTGCAT
CCCAATTTGCCCCACGGGTGCCACCTTCCAACGCCCAGATGGCATTGTGGTCGTCAACAACGAATGGTGCGTCGGTTGTG
GCTATTGTGTACAAGCTTGCCCCTACGATGCGCGCTTTATCAACCATGAGACCAACACAGCCGATAAATGCACCTTCTGC
GCACACAGGCTCGAAGCTGGACTCTTACCCGCCTGTGTCGAAACCTGTGTCGGCGAAGCAAGGGTGATTGGCGATCTAAA
CGATCCTCACAGCCACATAAGCCAGTTACTCAAGGCTAATCAAGCGGATATCAAGGTGCTCAAGCCCGATGCCAATACAG
CACCTAGAGTGTTTTACATCGGCATGAACAGCGCCTTTGAAGATCGCATTAACGGCCAAGCCCCCGTTCGCACCCTACTG
ACAGACTCTGGCGAGGAGATCCACCATGGTCATTAA

Upstream 100 bases:

>100_bases
CCTTCACTTTACCAGAATGCCCTTAAACCCAGCCCTTAGTCTCAGCCGCTGATATCTACGGGCATGAAGTGAGCTTTCTG
TAAGAGTGAAGGAAATCCCT

Downstream 100 bases:

>100_bases
AGAAATCTTTACTCCAGATATCGCCATCACTTGGCTGCCATGGGCAGTGCAATACTTCTTTTTAATGGGTTTGGCCTACG
CCAGTGTGTGGGTGGCGACC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDKSKRQFLGKMASVSVGAALIPVVQVQAQPAQTATTSKRYGMVIDLRRCVGCQACTVACTFENLPPLGQFRTTVQQYEV
SQQDSATPPAFLMLPRLCNHCENPPCIPICPTGATFQRPDGIVVVNNEWCVGCGYCVQACPYDARFINHETNTADKCTFC
AHRLEAGLLPACVETCVGEARVIGDLNDPHSHISQLLKANQADIKVLKPDANTAPRVFYIGMNSAFEDRINGQAPVRTLL
TDSGEEIHHGH

Sequences:

>Translated_251_residues
MDKSKRQFLGKMASVSVGAALIPVVQVQAQPAQTATTSKRYGMVIDLRRCVGCQACTVACTFENLPPLGQFRTTVQQYEV
SQQDSATPPAFLMLPRLCNHCENPPCIPICPTGATFQRPDGIVVVNNEWCVGCGYCVQACPYDARFINHETNTADKCTFC
AHRLEAGLLPACVETCVGEARVIGDLNDPHSHISQLLKANQADIKVLKPDANTAPRVFYIGMNSAFEDRINGQAPVRTLL
TDSGEEIHHGH
>Mature_251_residues
MDKSKRQFLGKMASVSVGAALIPVVQVQAQPAQTATTSKRYGMVIDLRRCVGCQACTVACTFENLPPLGQFRTTVQQYEV
SQQDSATPPAFLMLPRLCNHCENPPCIPICPTGATFQRPDGIVVVNNEWCVGCGYCVQACPYDARFINHETNTADKCTFC
AHRLEAGLLPACVETCVGEARVIGDLNDPHSHISQLLKANQADIKVLKPDANTAPRVFYIGMNSAFEDRINGQAPVRTLL
TDSGEEIHHGH

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=225, Percent_Identity=41.3333333333333, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI226510944, Length=202, Percent_Identity=35.1485148514851, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787872, Length=193, Percent_Identity=36.2694300518135, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=35.7512953367876, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787749, Length=210, Percent_Identity=30.4761904761905, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790326, Length=150, Percent_Identity=38, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787478, Length=98, Percent_Identity=45.9183673469388, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787740, Length=124, Percent_Identity=39.5161290322581, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789370, Length=205, Percent_Identity=27.8048780487805, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787963, Length=175, Percent_Identity=24.5714285714286, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788811, Length=150, Percent_Identity=34, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=31.2138728323699, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082179, Length=146, Percent_Identity=32.1917808219178, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367245, Length=163, Percent_Identity=30.0613496932515, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082180, Length=172, Percent_Identity=30.2325581395349, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27345; Mature: 27345

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
6.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSKRQFLGKMASVSVGAALIPVVQVQAQPAQTATTSKRYGMVIDLRRCVGCQACTVAC
CCCHHHHHHHHHHHHHHCHHHHHHEEECCCCCHHHHCCHHCCCEEEHHHHCCCCEEEEEE
TFENLPPLGQFRTTVQQYEVSQQDSATPPAFLMLPRLCNHCENPPCIPICPTGATFQRPD
EECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GIVVVNNEWCVGCGYCVQACPYDARFINHETNTADKCTFCAHRLEAGLLPACVETCVGEA
CEEEECCCEEEECCHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCE
RVIGDLNDPHSHISQLLKANQADIKVLKPDANTAPRVFYIGMNSAFEDRINGQAPVRTLL
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCHHHHHCCCCCCHHHHH
TDSGEEIHHGH
CCCCCCCCCCC
>Mature Secondary Structure
MDKSKRQFLGKMASVSVGAALIPVVQVQAQPAQTATTSKRYGMVIDLRRCVGCQACTVAC
CCCHHHHHHHHHHHHHHCHHHHHHEEECCCCCHHHHCCHHCCCEEEHHHHCCCCEEEEEE
TFENLPPLGQFRTTVQQYEVSQQDSATPPAFLMLPRLCNHCENPPCIPICPTGATFQRPD
EECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
GIVVVNNEWCVGCGYCVQACPYDARFINHETNTADKCTFCAHRLEAGLLPACVETCVGEA
CEEEECCCEEEECCHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCE
RVIGDLNDPHSHISQLLKANQADIKVLKPDANTAPRVFYIGMNSAFEDRINGQAPVRTLL
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCHHHHHCCCCCCHHHHH
TDSGEEIHHGH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]