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The map label for this gene is rpfG [H]

Identifier: 117918934

GI number: 117918934

Start: 556165

End: 557217

Strand: Reverse

Name: rpfG [H]

Synonym: Shewana3_0479

Alternate gene names: 117918934

Gene position: 557217-556165 (Counterclockwise)

Preceding gene: 117918937

Following gene: 117918933

Centisome position: 11.21

GC content: 49.67

Gene sequence:

>1053_bases
ATGACTTCACTTGTCACTAAACCCATATTATTAGTGGTGGATGATACACCCGATAATATTCAGATTATTCACGGGATCTT
GTCAGATCAATACACAATCCGCGCCGCAACCTCAGGTGAAAAGGCGCTTAAACTGGCCAGCACCCATCCCCTCCCAGAGC
TTATCTTACTCGATGTGATGATGCCTGAGATGGATGGCTTCGAGACCTGTCGTAGGCTTAAGCGAGACCCCATCACCAGC
AAGATCCCTGTGATCTTTGTGACCGCCAAAACCGATACCATAGATGAGCAAACAGGCTTTGAACTCGGTGCAGTGGACTA
TATTTCCAAACCCATTAGCCCTGCGGTACTCAAGGTGCGCACTCAAACCCATTTAGCCTTGAGTAATCAGGCAAGGCAGC
TAGAAGCCCTAGTACAGCAACGGACACAAGAGTTGGAATTGACTCGCCATAAAATTATCCAAAAACTGGGGCTGGCGGCG
GAGTATCGCGATAACGAAACGGGCCTACATATTACTAGAATGAGCCACTACGGCCGCATTTTGGCCCAATATGTGTGTGA
TTCCCAAGCTTGGTGTGAGATGTATTTTACCGCGCTCCCGATGCACGATATTGGCAAAATCGGCATCCCAGATTCGATCC
TGTCTAAGCCGGGAAAATTAACCGATGATGAGCGACGCCAAATGCAGCAACACGCTTCCTTTGGCGCGCAGATCCTCGGT
GATGACGATGACCCGCTGTTATCCCTCGCCAAGGAGATCGCGCTCTATCATCACGAACGTTGGGACGGAACAGGTTATCC
CCACAGGCTAGCAGGCGAGCAAATTCCATTAAGTGCCCGCATTGCCGCCATCGCCGATGTCTTCGATGCACTCACCTCAG
ACCGCCCCTATAAAGTGGCTTGGAGCACACAAAAGGCATTTGATTATATTGAAGAAAATGCTGGCACTCAGTTTGATCCT
AAACTCACTCGCGCCTTTATCGAGTGCAAAGCGCAGGTACTAGAAGTACAGCAACGCTTTGCAGAACCCAAAACAACCAG
TGTTGTCGCTTGA

Upstream 100 bases:

>100_bases
GCCTAGTTTGGCACTGGCATTAACGGGGACGAATTGGGGTTTTCTCAGTCTCGCCACCGGATTAATACGGGAAACAAACC
GACTCAGTAGAGGCGAAACC

Downstream 100 bases:

>100_bases
ATGCCTGACTAAAGCGGGAGCAAGAGGTAGACTCATAGTCATAGGGGCTAGATATGGGGCTAATAGATGAATCCAGCAAA
GGTAGTGCGCAACGAGGTAA

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTSLVTKPILLVVDDTPDNIQIIHGILSDQYTIRAATSGEKALKLASTHPLPELILLDVMMPEMDGFETCRRLKRDPITS
KIPVIFVTAKTDTIDEQTGFELGAVDYISKPISPAVLKVRTQTHLALSNQARQLEALVQQRTQELELTRHKIIQKLGLAA
EYRDNETGLHITRMSHYGRILAQYVCDSQAWCEMYFTALPMHDIGKIGIPDSILSKPGKLTDDERRQMQQHASFGAQILG
DDDDPLLSLAKEIALYHHERWDGTGYPHRLAGEQIPLSARIAAIADVFDALTSDRPYKVAWSTQKAFDYIEENAGTQFDP
KLTRAFIECKAQVLEVQQRFAEPKTTSVVA

Sequences:

>Translated_350_residues
MTSLVTKPILLVVDDTPDNIQIIHGILSDQYTIRAATSGEKALKLASTHPLPELILLDVMMPEMDGFETCRRLKRDPITS
KIPVIFVTAKTDTIDEQTGFELGAVDYISKPISPAVLKVRTQTHLALSNQARQLEALVQQRTQELELTRHKIIQKLGLAA
EYRDNETGLHITRMSHYGRILAQYVCDSQAWCEMYFTALPMHDIGKIGIPDSILSKPGKLTDDERRQMQQHASFGAQILG
DDDDPLLSLAKEIALYHHERWDGTGYPHRLAGEQIPLSARIAAIADVFDALTSDRPYKVAWSTQKAFDYIEENAGTQFDP
KLTRAFIECKAQVLEVQQRFAEPKTTSVVA
>Mature_349_residues
TSLVTKPILLVVDDTPDNIQIIHGILSDQYTIRAATSGEKALKLASTHPLPELILLDVMMPEMDGFETCRRLKRDPITSK
IPVIFVTAKTDTIDEQTGFELGAVDYISKPISPAVLKVRTQTHLALSNQARQLEALVQQRTQELELTRHKIIQKLGLAAE
YRDNETGLHITRMSHYGRILAQYVCDSQAWCEMYFTALPMHDIGKIGIPDSILSKPGKLTDDERRQMQQHASFGAQILGD
DDDPLLSLAKEIALYHHERWDGTGYPHRLAGEQIPLSARIAAIADVFDALTSDRPYKVAWSTQKAFDYIEENAGTQFDPK
LTRAFIECKAQVLEVQQRFAEPKTTSVVA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=129, Percent_Identity=37.984496124031, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789809, Length=158, Percent_Identity=31.6455696202532, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=150, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=104, Percent_Identity=34.6153846153846, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39250; Mature: 39119

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLVTKPILLVVDDTPDNIQIIHGILSDQYTIRAATSGEKALKLASTHPLPELILLDVM
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHEEHHCCCCCHHHHHHHHH
MPEMDGFETCRRLKRDPITSKIPVIFVTAKTDTIDEQTGFELGAVDYISKPISPAVLKVR
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCHHHHHHCCCCCHHEEEE
TQTHLALSNQARQLEALVQQRTQELELTRHKIIQKLGLAAEYRDNETGLHITRMSHYGRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEHHHHHHH
LAQYVCDSQAWCEMYFTALPMHDIGKIGIPDSILSKPGKLTDDERRQMQQHASFGAQILG
HHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEC
DDDDPLLSLAKEIALYHHERWDGTGYPHRLAGEQIPLSARIAAIADVFDALTSDRPYKVA
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
WSTQKAFDYIEENAGTQFDPKLTRAFIECKAQVLEVQQRFAEPKTTSVVA
ECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSLVTKPILLVVDDTPDNIQIIHGILSDQYTIRAATSGEKALKLASTHPLPELILLDVM
CCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHEEHHCCCCCHHHHHHHHH
MPEMDGFETCRRLKRDPITSKIPVIFVTAKTDTIDEQTGFELGAVDYISKPISPAVLKVR
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCHHHHHHCCCCCHHEEEE
TQTHLALSNQARQLEALVQQRTQELELTRHKIIQKLGLAAEYRDNETGLHITRMSHYGRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEHHHHHHH
LAQYVCDSQAWCEMYFTALPMHDIGKIGIPDSILSKPGKLTDDERRQMQQHASFGAQILG
HHHHHHCCHHHHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEC
DDDDPLLSLAKEIALYHHERWDGTGYPHRLAGEQIPLSARIAAIADVFDALTSDRPYKVA
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
WSTQKAFDYIEENAGTQFDPKLTRAFIECKAQVLEVQQRFAEPKTTSVVA
ECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]