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The map label for this gene is purD [H]

Identifier: 117918897

GI number: 117918897

Start: 514383

End: 515681

Strand: Reverse

Name: purD [H]

Synonym: Shewana3_0440

Alternate gene names: 117918897

Gene position: 515681-514383 (Counterclockwise)

Preceding gene: 117918898

Following gene: 117918896

Centisome position: 10.37

GC content: 53.27

Gene sequence:

>1299_bases
ATGAAAGTATTAGTTATTGGTGGCGGCGGCCGCGAACATGCCCTAGCTTGGAAAGCGGCACAATCCCCACAGGTAGAGCT
GGTTTATGTTGCTCCGGGTAACGCTGGCACAGCCCTCGAGCCGAAGTTAGAAAACCTCAACATCAGCGCGACGGACATTC
CTGCCCTGCTCGATTTTGCTCAAACCAATCAGATCGAACTCACCATCGTTGGCCCAGAAGCGCCGCTGGTATTGGGTGTG
GTCGATGCCTTTAACGCTGCGGGTCTACCCATTTTTGGCCCAACCAAAGCGGCAGCACAGTTAGAAGGCTCTAAGGCGTT
CACTAAGGACTTCTTAGCACGCCACAATATCCCAACTGCGGGTTATAAAAACTGTACCGAAATCCAAGATGCTAAAGCCT
TCGTGCGTGAACTAACGGGCAAGACAGGTTACCCAGTCGTAATCAAGGCCGACGGTTTAGCCGCAGGTAAAGGCGTGATC
ATCGCCCAAGATCAAGCCGAAGCCGATGCCGCCATCGAAGATATGCTCGCAGGCAACAAGTTTGGCGATGCCGGTTCTCG
CGTGGTTATCGAAGAATTCTTAAAAGGCGAAGAAGCCAGCTTTATCGTGATGGTTGATGGTAAAAATATCCTCGCCATGG
CGACCAGCCAAGACCATAAAGCCCGTGATAATGCCGACCACGGTCCTAACACTGGCGGAATGGGCGCTTACTCACCTGCA
CCAGTCGTTACCCAAAGCGTGCACGATTGGACCATCGCTAACGTTATCCGCCCAACGGTTGATGGCATGGCGGCTGAAGG
CAATGTTTACACTGGCTTCCTGTATGCGGGTCTGATGATCGCACCAGACGGCAGCGCCAAAGTACTGGAATACAACTGCC
GCTTTGGCGACCCAGAAACCCAACCTATTATGATGCGCCTCAAGTCAGATCTGGTTGAGCTGTGTTTAGCCGCGACCCGT
GGCGAGCTGGATAAAGTTACCGCCGAGTACGACTCACGCGCCGCAGTCGGTGTTGTATTAGCCGCAGGCGGTTACCCAGA
CGACTACCGCAAGGGCGATGTGATCCAAGGTTTAAGCCTAGGTAATCACGATGCCAAAGTCTTCCACGCGGGTACTGAGA
TGAAAGACGGTCATGTAGTTACTAACGGCGGCCGCGTTCTATGCGCTACAGCCCTAGGACACACTGTGACCGAAGCACAA
AAAGCTGCTTATCTGCTGGTTGATGAAATCCACTGGGATGACGTGTATTTCCGTACCGATATCGGTTATCGCGCCATTGC
CCGCGAACAACAGGGCTAA

Upstream 100 bases:

>100_bases
CCTACAGCCAGCACGCTATGTGCTGGCTGTTTCGTTTATCGCACTCTGTAGCGCTAAGAATCTAACCACAAGAATTTAAA
ACTCAAAGGGATGACTTAAC

Downstream 100 bases:

>100_bases
GCTTTAATTCATTTAAAAAAACCGGCTATGCGCCGGTTTTTTTATGACTTAAATTTGACAAGCGTCAATTTTAAGTTAAT
GATTTATATCGATTTAAACA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKVLVIGGGGREHALAWKAAQSPQVELVYVAPGNAGTALEPKLENLNISATDIPALLDFAQTNQIELTIVGPEAPLVLGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEIQDAKAFVRELTGKTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIEDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQSVHDWTIANVIRPTVDGMAAEGNVYTGFLYAGLMIAPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDSRAAVGVVLAAGGYPDDYRKGDVIQGLSLGNHDAKVFHAGTEMKDGHVVTNGGRVLCATALGHTVTEAQ
KAAYLLVDEIHWDDVYFRTDIGYRAIAREQQG

Sequences:

>Translated_432_residues
MKVLVIGGGGREHALAWKAAQSPQVELVYVAPGNAGTALEPKLENLNISATDIPALLDFAQTNQIELTIVGPEAPLVLGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEIQDAKAFVRELTGKTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIEDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQSVHDWTIANVIRPTVDGMAAEGNVYTGFLYAGLMIAPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDSRAAVGVVLAAGGYPDDYRKGDVIQGLSLGNHDAKVFHAGTEMKDGHVVTNGGRVLCATALGHTVTEAQ
KAAYLLVDEIHWDDVYFRTDIGYRAIAREQQG
>Mature_432_residues
MKVLVIGGGGREHALAWKAAQSPQVELVYVAPGNAGTALEPKLENLNISATDIPALLDFAQTNQIELTIVGPEAPLVLGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEIQDAKAFVRELTGKTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIEDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQSVHDWTIANVIRPTVDGMAAEGNVYTGFLYAGLMIAPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDSRAAVGVVLAAGGYPDDYRKGDVIQGLSLGNHDAKVFHAGTEMKDGHVVTNGGRVLCATALGHTVTEAQ
KAAYLLVDEIHWDDVYFRTDIGYRAIAREQQG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=436, Percent_Identity=47.9357798165138, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=436, Percent_Identity=47.9357798165138, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=436, Percent_Identity=47.9357798165138, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=436, Percent_Identity=47.9357798165138, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=433, Percent_Identity=72.0554272517321, Blast_Score=621, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.2735849056604, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=43.4004474272931, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=45.3917050691244, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=45.3917050691244, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45697; Mature: 45697

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGGGREHALAWKAAQSPQVELVYVAPGNAGTALEPKLENLNISATDIPALLDFA
CEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QTNQIELTIVGPEAPLVLGVVDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEEEEHHHCCCCCCEECCCCHHHHCCCCHHHHHHHHHHCCCCCC
GYKNCTEIQDAKAFVRELTGKTGYPVVIKADGLAAGKGVIIAQDQAEADAAIEDMLAGNK
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGDAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNADHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQSVHDWTIANVIRPTVDGMAAEGNVYTGFLYAGLMIAPDGSAKVLEYNCRFGDPET
CHHHCCCCCCHHHHHHHHHHCCEECCCCEEEEEEEECEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAATRGELDKVTAEYDSRAAVGVVLAAGGYPDDYRKGDVIQGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEECCCC
GNHDAKVFHAGTEMKDGHVVTNGGRVLCATALGHTVTEAQKAAYLLVDEIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHCEEEEEEEEECCCEEEEEC
IGYRAIAREQQG
CCHHHHHHCCCC
>Mature Secondary Structure
MKVLVIGGGGREHALAWKAAQSPQVELVYVAPGNAGTALEPKLENLNISATDIPALLDFA
CEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QTNQIELTIVGPEAPLVLGVVDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEEEEHHHCCCCCCEECCCCHHHHCCCCHHHHHHHHHHCCCCCC
GYKNCTEIQDAKAFVRELTGKTGYPVVIKADGLAAGKGVIIAQDQAEADAAIEDMLAGNK
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGDAGSRVVIEEFLKGEEASFIVMVDGKNILAMATSQDHKARDNADHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQSVHDWTIANVIRPTVDGMAAEGNVYTGFLYAGLMIAPDGSAKVLEYNCRFGDPET
CHHHCCCCCCHHHHHHHHHHCCEECCCCEEEEEEEECEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAATRGELDKVTAEYDSRAAVGVVLAAGGYPDDYRKGDVIQGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEECCCC
GNHDAKVFHAGTEMKDGHVVTNGGRVLCATALGHTVTEAQKAAYLLVDEIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHCEEEEEEEEECCCEEEEEC
IGYRAIAREQQG
CCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]