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The map label for this gene is purH [H]

Identifier: 117918898

GI number: 117918898

Start: 515787

End: 517385

Strand: Reverse

Name: purH [H]

Synonym: Shewana3_0441

Alternate gene names: 117918898

Gene position: 517385-515787 (Counterclockwise)

Preceding gene: 117918901

Following gene: 117918897

Centisome position: 10.41

GC content: 52.85

Gene sequence:

>1599_bases
ATGACTGTTGCAAATAATGCCAGACCCATTCGTCGCGCGCTGTTAAGCGTTTCAGATAAAACCGGAATTCTCGAATTCGC
CAAAGCATTACACGCCCAAGGCGTTGAACTGCTGTCAACGGGCGGCACCGCTCGCTTGTTAGCGGATAACGGCGTGCCTG
TTATCGAAGTATCTGACTATACAGGACACCCTGAGATCATGGATGGTCGCGTTAAGACCCTGCACCCTAAAGTGCATGGT
GGCATTTTGGCGCGTCGCGGTCTTGATGAAAATGTCATGGCTGCCAACAACATCAATGCAATCGATCTGGTTGCGGTTAA
CCTCTACCCCTTTGCCGATACCGTTGCTAAAGCCGGTTGCACCCTAGAAGATGCGATTGAAAACATCGACATCGGTGGCC
CGACTATGGTGCGCGCCGCGGCGAAAAACCATAAAGACGTGACTATCGTGGTAAATGCGGCCGACTATAACCGCGTATTA
GCCGAAATGGCCGCCAACAATGGCAGCACGACTCACACGACCCGTTTCGATTTAGCGATTGCTGCCTTCGAACACACTGC
GGGTTACGATGGCATGATCGCCAACTACTTCGGCACTATGGTTCCTGCACACAGCACTGACGAGTGCTTCGAAGATTCTA
AGTTCCCACGCACCTTCAACACCCAATTAGTGAAGAAGCAAGATCTACGTTACGGTGAAAACAGCCACCAAACTGCGGCC
TTCTATGTCGACACTAAGATCGACGAAGCCTCTGTTGCGACAGCCGTCCAGCTGCAAGGTAAGGCACTGTCTTACAACAA
CATCGCCGATACCGATGCCGCCCTTGAGTGTGTGAAAGAGTTCAGCGAGCCAGCCTGCGTTATCGTTAAACACGCTAACC
CATGTGGTGTTGCACTGGGTAAAGACTTGCTCGATGCCTATAACCGCGCCTATCAAACTGATCCAACTTCAGCCTTCGGC
GGCATTATCGCCTTCAACGGCGAGTTAGATGCAGCAACGGCTAGCGCTATCGTTGAGCGTCAATTCGTTGAAGTGATTAT
TGCGCCAGTCGTGAGCCAAGGCGCACGCGATGTAGTGGCCAAGAAAACCAACGTGCGTCTGTTAGAGTGCGGTCAATGGG
ATACTAAGACCAAGACCTTAGACTATAAGCGCGTGAACGGTGGCCTGCTGGTGCAAGACCGCGATCAAGGCATGGTTGGT
TTAGATGACATTAAAGTCGTGACTAAACGTCAACCGACCGAGAGCGAGCTGAAGGACTTAATGTTCTGCTGGAAAGTGGC
TAAGTTCGTTAAATCTAACGCCATTGTTTACGCTAAAGACGGTATGACCATCGGTGTCGGCGCCGGCCAAATGAGCCGCG
TCTACAGCGCTAAGATTGCCGGTATCAAGGCGGCCGATGAAGGCCTAGAAGTGGTTAACTCTGTGATGGCGTCCGATGCC
TTCTTCCCATTCCGCGACGGTATCGATGCCGCAGCGGCGGCGGGCATCAGCTGCATCATCCAGCCAGGTGGCTCAATGCG
CGATGCTGAAATCATCGCTGCTGCCGACGAGCACGGCATGGCCATGGTAATGACGGGCATGCGCCACTTCCGTCACTAA

Upstream 100 bases:

>100_bases
ATAAGTAGGACCAAGGGGCTTTTGGAAAGCAGAACCCGATAGAAGTTGTTCTGTTTTCCGAAAGATCCTTGAAACTAAAA
TAACTGGACCCTGTACCCAA

Downstream 100 bases:

>100_bases
TGCCACCTACAGCCAGCACGCTATGTGCTGGCTGTTTCGTTTATCGCACTCTGTAGCGCTAAGAATCTAACCACAAGAAT
TTAAAACTCAAAGGGATGAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTVANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHG
GILARRGLDENVMAANNINAIDLVAVNLYPFADTVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYNRVL
AEMAANNGSTTHTTRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFEDSKFPRTFNTQLVKKQDLRYGENSHQTAA
FYVDTKIDEASVATAVQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFG
GIIAFNGELDAATASAIVERQFVEVIIAPVVSQGARDVVAKKTNVRLLECGQWDTKTKTLDYKRVNGGLLVQDRDQGMVG
LDDIKVVTKRQPTESELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH

Sequences:

>Translated_532_residues
MTVANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHG
GILARRGLDENVMAANNINAIDLVAVNLYPFADTVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYNRVL
AEMAANNGSTTHTTRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFEDSKFPRTFNTQLVKKQDLRYGENSHQTAA
FYVDTKIDEASVATAVQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFG
GIIAFNGELDAATASAIVERQFVEVIIAPVVSQGARDVVAKKTNVRLLECGQWDTKTKTLDYKRVNGGLLVQDRDQGMVG
LDDIKVVTKRQPTESELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
>Mature_531_residues
TVANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGG
ILARRGLDENVMAANNINAIDLVAVNLYPFADTVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYNRVLA
EMAANNGSTTHTTRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFEDSKFPRTFNTQLVKKQDLRYGENSHQTAAF
YVDTKIDEASVATAVQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFGG
IIAFNGELDAATASAIVERQFVEVIIAPVVSQGARDVVAKKTNVRLLECGQWDTKTKTLDYKRVNGGLLVQDRDQGMVGL
DDIKVVTKRQPTESELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDAF
FPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=38.9344262295082, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=74.2857142857143, Blast_Score=822, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=509, Percent_Identity=33.9882121807466, Blast_Score=239, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=339, Percent_Identity=27.4336283185841, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=612, Percent_Identity=33.3333333333333, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.4197530864198, Blast_Score=266, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24649832, Length=613, Percent_Identity=35.7259380097879, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57039; Mature: 56908

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDY
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCC
TGHPEIMDGRVKTLHPKVHGGILARRGLDENVMAANNINAIDLVAVNLYPFADTVAKAGC
CCCCCCCCCCEEEECCHHHCCHHHHCCCCCCCEECCCCCEEEEEEEEEECCHHHHHHCCC
TLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYNRVLAEMAANNGSTTHTTRFDLAI
CHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEEEE
AAFEHTAGYDGMIANYFGTMVPAHSTDECFEDSKFPRTFNTQLVKKQDLRYGENSHQTAA
EEEHHCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
FYVDTKIDEASVATAVQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALG
EEEECCCCCHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
KDLLDAYNRAYQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPVVSQGARDVVA
HHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KKTNVRLLECGQWDTKTKTLDYKRVNGGLLVQDRDQGMVGLDDIKVVTKRQPTESELKDL
CCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHH
MFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
CCCCCCCCCHHHHCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TVANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDY
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCC
TGHPEIMDGRVKTLHPKVHGGILARRGLDENVMAANNINAIDLVAVNLYPFADTVAKAGC
CCCCCCCCCCEEEECCHHHCCHHHHCCCCCCCEECCCCCEEEEEEEEEECCHHHHHHCCC
TLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYNRVLAEMAANNGSTTHTTRFDLAI
CHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEEEE
AAFEHTAGYDGMIANYFGTMVPAHSTDECFEDSKFPRTFNTQLVKKQDLRYGENSHQTAA
EEEHHCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
FYVDTKIDEASVATAVQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALG
EEEECCCCCHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
KDLLDAYNRAYQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPVVSQGARDVVA
HHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KKTNVRLLECGQWDTKTKTLDYKRVNGGLLVQDRDQGMVGLDDIKVVTKRQPTESELKDL
CCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHH
MFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
CCCCCCCCCHHHHCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]