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The map label for this gene is nemR [H]

Identifier: 117918719

GI number: 117918719

Start: 293767

End: 294363

Strand: Reverse

Name: nemR [H]

Synonym: Shewana3_0262

Alternate gene names: 117918719

Gene position: 294363-293767 (Counterclockwise)

Preceding gene: 117918720

Following gene: 117918718

Centisome position: 5.92

GC content: 49.08

Gene sequence:

>597_bases
ATGAAAACAGAAACCCAATCGACGCGTCAGCATATTCTCGACGTTGGATACTCACTGATCCTCAAACAAGGCTTTTCGTG
CCTAGGTTTAGCCCAATTACTCAAGGCAGCCCAAGTACCTAAAGGCTCGTTTTATCACTACTTTAAATCCAAAGAGCAAT
TCGGTGAGGCCTTGTTAGCAGGGTATTTTGAGCAATACCAAGCAGAACTCGATAGCCTGCTCAACGAGACTCAACTCTCA
GGCTTTGAGCGCCAAATGCAGTACTGGCAAAAATGGTTGCACGTGCAGCAGGACGGTTGTGTCGATCAAAAATGCCTAGT
CGTTAAGTTAAGTGCAGAGGTAGCCGATCTCTCTGAGGCCATGCGCCTTGTGCTTTTAAAAGGCTCGGCTGGCATCATCG
AGCGCCTAACCCAGTGCATTCAGGCGGGGATTGCCGATAAATCCATCTGTGAACAGGATGCCCAGGCAACGGCAGAGTTG
CTGTATCACATGTGGCTTGGCGCCAGTTTGATGAATAAACTCGGCCACAGCCATGCCGCTTTAGCGCGCGCCCTTGCAGT
GACCGAATCTATTTTAAAGACCAAAACCATGGGTTAA

Upstream 100 bases:

>100_bases
TGCTTAATCAACTTAGGCATTCCTCAATCAGTTGCTCTCCACTATTGATATAAATTAGAACACTAGACGACTGGTCTAAT
ATATTTTATGCTCCTTCGCC

Downstream 100 bases:

>100_bases
TGCCTGCAACGCTGGCACACCAATGCATCAGCAACCCGTTAATGAACAATGAAATATCGCCTCATCGGCCTGATTTATCA
TTTAGTAGCCAACCAATCTA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLAGYFEQYQAELDSLLNETQLS
GFERQMQYWQKWLHVQQDGCVDQKCLVVKLSAEVADLSEAMRLVLLKGSAGIIERLTQCIQAGIADKSICEQDAQATAEL
LYHMWLGASLMNKLGHSHAALARALAVTESILKTKTMG

Sequences:

>Translated_198_residues
MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLAGYFEQYQAELDSLLNETQLS
GFERQMQYWQKWLHVQQDGCVDQKCLVVKLSAEVADLSEAMRLVLLKGSAGIIERLTQCIQAGIADKSICEQDAQATAEL
LYHMWLGASLMNKLGHSHAALARALAVTESILKTKTMG
>Mature_198_residues
MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLAGYFEQYQAELDSLLNETQLS
GFERQMQYWQKWLHVQQDGCVDQKCLVVKLSAEVADLSEAMRLVLLKGSAGIIERLTQCIQAGIADKSICEQDAQATAEL
LYHMWLGASLMNKLGHSHAALARALAVTESILKTKTMG

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=199, Percent_Identity=41.7085427135678, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22047; Mature: 22047

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GYFEQYQAELDSLLNETQLSGFERQMQYWQKWLHVQQDGCVDQKCLVVKLSAEVADLSEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEHHHHHHHHHHH
MRLVLLKGSAGIIERLTQCIQAGIADKSICEQDAQATAELLYHMWLGASLMNKLGHSHAA
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LARALAVTESILKTKTMG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GYFEQYQAELDSLLNETQLSGFERQMQYWQKWLHVQQDGCVDQKCLVVKLSAEVADLSEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEHHHHHHHHHHH
MRLVLLKGSAGIIERLTQCIQAGIADKSICEQDAQATAELLYHMWLGASLMNKLGHSHAA
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LARALAVTESILKTKTMG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]