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The map label for this gene is moeB [H]

Identifier: 117918588

GI number: 117918588

Start: 145498

End: 146259

Strand: Reverse

Name: moeB [H]

Synonym: Shewana3_0130

Alternate gene names: 117918588

Gene position: 146259-145498 (Counterclockwise)

Preceding gene: 117918589

Following gene: 117918587

Centisome position: 2.94

GC content: 51.84

Gene sequence:

>762_bases
ATGAATGAACAACTAGAGATCCTCAGCGACGGCGAACTCACCCGCTACAGTCGCCAAATCTCAATCAAGGCGATGGATAT
CGACGGCCAAGAGCGCTTAAAACTCGCCAGAGTCTTGATGATTGGCGCCGGCGGTTTAGGCTGCGCGGCGGGGCAATATC
TCACCGTTGCGGGCATTGGCGAGCTGACTCTAGTGGATTTCGATACGGTCGAACTCTCTAATTTGCAGCGCCAAGTGCTG
CATCAGGATGCGACTATCGGCCAACCTAAGGTCGAGTCGGCCAAGCAGAGCCTTAACCGACTCAATCCTCACGTTAAAAT
CAATACCATCAATGCGGTATTGGATGACCATGAGATCGACGCCTTAGTCGCCAGCCACAGTATCGTGGTGGATTGCACCG
ATAATGTCAGTGTGCGTGAGCAGTTAAATCAGAGCTGTTTTAAGCACAAGATCCCGCTGGTCTCAGCCGCCGCAATTCGT
ATGGAAGGCATGGTTACCATCTTCGACTATCAAGCGCAAACGCCTTGTTATCATTGCTTTAGTTCACTCTTTGGCGAGCA
ACAACTCAGCTGCGTCGAGTCGGGCATTCTCGCCCCCGTGGTCGGCATGGTCGGCTGCTTACAGGCGGTTGAAGCCATTA
AAGTGATTGCGGGGATGGGCAAGACACTCGCAGGACGAATATTGATGATCGATGCCATGACCATGGAATTTCGCGAAATG
AAACTCCCTAAACAACCCCGCTGCAAGATTTGTGGTGAATAA

Upstream 100 bases:

>100_bases
ATTGTTTCGTACTGCTGGAGCAATTCCAAGGTGACACCCCAGTGGGCACGCAAGTGACGGTTGAACCTTTTAATTCAGTG
CTCTGCTAAGGTACGCCTCG

Downstream 100 bases:

>100_bases
CAGAGTAACCTTCAGCACTTAGCACGCTTTCTACAATTAAGGATATAAAAATCAATGGATTCTGAAAACAGCGTATTCGA
CCGCCGCACTACCGATGACA

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNEQLEILSDGELTRYSRQISIKAMDIDGQERLKLARVLMIGAGGLGCAAGQYLTVAGIGELTLVDFDTVELSNLQRQVL
HQDATIGQPKVESAKQSLNRLNPHVKINTINAVLDDHEIDALVASHSIVVDCTDNVSVREQLNQSCFKHKIPLVSAAAIR
MEGMVTIFDYQAQTPCYHCFSSLFGEQQLSCVESGILAPVVGMVGCLQAVEAIKVIAGMGKTLAGRILMIDAMTMEFREM
KLPKQPRCKICGE

Sequences:

>Translated_253_residues
MNEQLEILSDGELTRYSRQISIKAMDIDGQERLKLARVLMIGAGGLGCAAGQYLTVAGIGELTLVDFDTVELSNLQRQVL
HQDATIGQPKVESAKQSLNRLNPHVKINTINAVLDDHEIDALVASHSIVVDCTDNVSVREQLNQSCFKHKIPLVSAAAIR
MEGMVTIFDYQAQTPCYHCFSSLFGEQQLSCVESGILAPVVGMVGCLQAVEAIKVIAGMGKTLAGRILMIDAMTMEFREM
KLPKQPRCKICGE
>Mature_253_residues
MNEQLEILSDGELTRYSRQISIKAMDIDGQERLKLARVLMIGAGGLGCAAGQYLTVAGIGELTLVDFDTVELSNLQRQVL
HQDATIGQPKVESAKQSLNRLNPHVKINTINAVLDDHEIDALVASHSIVVDCTDNVSVREQLNQSCFKHKIPLVSAAAIR
MEGMVTIFDYQAQTPCYHCFSSLFGEQQLSCVESGILAPVVGMVGCLQAVEAIKVIAGMGKTLAGRILMIDAMTMEFREM
KLPKQPRCKICGE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=249, Percent_Identity=43.3734939759036, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI4885649, Length=145, Percent_Identity=31.0344827586207, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI23510338, Length=163, Percent_Identity=27.6073619631902, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI23510340, Length=163, Percent_Identity=27.6073619631902, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI38045948, Length=164, Percent_Identity=29.8780487804878, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38045942, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI38045944, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787048, Length=246, Percent_Identity=62.1951219512195, Blast_Score=342, Evalue=1e-95,
Organism=Escherichia coli, GI87082356, Length=245, Percent_Identity=37.1428571428571, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1789177, Length=131, Percent_Identity=30.5343511450382, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=249, Percent_Identity=37.3493975903614, Blast_Score=190, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=151, Percent_Identity=26.4900662251656, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI86565433, Length=163, Percent_Identity=26.3803680981595, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI86565431, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=35.1778656126482, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6322639, Length=162, Percent_Identity=28.3950617283951, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=153, Percent_Identity=26.797385620915, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325438, Length=158, Percent_Identity=27.2151898734177, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325323, Length=72, Percent_Identity=41.6666666666667, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582879, Length=247, Percent_Identity=42.5101214574899, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=30.2631578947368, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28573937, Length=162, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129973, Length=76, Percent_Identity=46.0526315789474, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641311, Length=257, Percent_Identity=26.4591439688716, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 27620; Mature: 27620

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQLEILSDGELTRYSRQISIKAMDIDGQERLKLARVLMIGAGGLGCAAGQYLTVAGIG
CCCCEEEECCCCHHEEHEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
ELTLVDFDTVELSNLQRQVLHQDATIGQPKVESAKQSLNRLNPHVKINTINAVLDDHEID
EEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHH
ALVASHSIVVDCTDNVSVREQLNQSCFKHKIPLVSAAAIRMEGMVTIFDYQAQTPCYHCF
HHEECCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHHHHHH
SSLFGEQQLSCVESGILAPVVGMVGCLQAVEAIKVIAGMGKTLAGRILMIDAMTMEFREM
HHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHHHHHH
KLPKQPRCKICGE
CCCCCCCCCCCCC
>Mature Secondary Structure
MNEQLEILSDGELTRYSRQISIKAMDIDGQERLKLARVLMIGAGGLGCAAGQYLTVAGIG
CCCCEEEECCCCHHEEHEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
ELTLVDFDTVELSNLQRQVLHQDATIGQPKVESAKQSLNRLNPHVKINTINAVLDDHEID
EEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHH
ALVASHSIVVDCTDNVSVREQLNQSCFKHKIPLVSAAAIRMEGMVTIFDYQAQTPCYHCF
HHEECCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHHHHHH
SSLFGEQQLSCVESGILAPVVGMVGCLQAVEAIKVIAGMGKTLAGRILMIDAMTMEFREM
HHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHHHHHH
KLPKQPRCKICGE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]