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The map label for this gene is dosC [H]

Identifier: 117918481

GI number: 117918481

Start: 26815

End: 28026

Strand: Reverse

Name: dosC [H]

Synonym: Shewana3_0022

Alternate gene names: 117918481

Gene position: 28026-26815 (Counterclockwise)

Preceding gene: 117918482

Following gene: 117918480

Centisome position: 0.56

GC content: 44.72

Gene sequence:

>1212_bases
ATGCTGCAAACGGAACAAACGCTTTTCGAACAAATGCGGATCACTGAGCTTGAGATTGATTTTCGCAAGTCCTTGTTCTC
TTTTACCTTAGCCGATGTCAGAGCACTGCAATCCTTCAAACCTGTGATTGAAGAGAACATAGATAAAATCGTTGATGACT
TTTACGGTCTACAAACCAGTGTGTCCGAGATTGCTCTGCTGATCGGAGACTCCGATACCCTAGCTCGTTTACGTACCGCA
CAGAGACGTTATGTGCTCGACCTGTTTAATGGTGTGTACGATTTAGAATATGTAAACAACCGCCTGCGGATTGGATTGGT
ACATAAGCGTATCGGCGTTGAACCTAAACTCTACCTTTCTGCCGTTCATACTCTAAAAGAGTTGATTTATGCCGAGATAA
ATAACTCGGTTGCTGATGACTTGCAAAATGAACGCATTCGAGTCGCCATAGATAAACTTGTTCTCTTCGACGTCACCCTC
GTCTTCGATACCTATATTCGAAGCCTAGTATCCGAAATTGAGAACGCCAAAGACAAGGCTGAACGTTACGCACAGAGCAT
GGAGAGCAAAGTCAAAGAACGTACACAACAGCTCGAAGAATTATCTCAAACCGACCCGTTAACGGGGCTATTAAATGTGC
GACACCTGCAAGAAATTGGCACTCGCATTTTAAGAGCAGCTCAGAGACGAGCCGAACCAGTTAGCGTAATCTACTTAGAT
GTTGATGATTTCAAATCCTTTAATGATACCCAAGGCCATCGCTCTGGAGACGAAGTGCTTTGCGCCGTCGCGCAGGCCAT
CAAAGAAAGCACCCGCGTAGAAGATCATAGTTTTCGCTACGGTGGCGATGAGTTCTGCATCATCATGCCCAATTGTCGAG
AAGACCAAGCCAGAGACAACTTTATCGAGCGCTTTAATCAGAATATTAAACTGCACCTAAACAACATCTCCCTCAGTTAC
GGTATTGTTGATACAGGCCCCTATGATTACGATGATGCCAATACCTTGATCCATAAAGCGGATCAAAAAATGTATAGCTA
TAAACGTGCTGCCAAGTTGCAGCAGCAAGGCTCAGTGTCGATTGCGGCAAATGATATAAAATCCGATATGCCTCAGTCCG
CTTCTCAAGTAGGAACACCCCAAGCAGCAATTACGCCAACCATAGTGCATAGCATTCAACAACCTCAGGGAATAAACAAA
GTTGCCGAGTAA

Upstream 100 bases:

>100_bases
ATAAATATCCTTAATGCACTTCTCCGCATTTGACCGTCATTACGACACTGCTAAGAAGCATGGATGCGTTTCATTCTTTA
GGCTACGAGAGGGATGTTCA

Downstream 100 bases:

>100_bases
TAGAAATGTTCCACGTGAAACATTCAGCTGGCGGTAAACAAATTGGCAAGAGCAATGCCTAGCCCCATCACAAATTCAAG
CCATCAACTTGGTTTATCCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLQTEQTLFEQMRITELEIDFRKSLFSFTLADVRALQSFKPVIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAEINNSVADDLQNERIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAERYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQEIGTRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLCAVAQAIKESTRVEDHSFRYGGDEFCIIMPNCREDQARDNFIERFNQNIKLHLNNISLSY
GIVDTGPYDYDDANTLIHKADQKMYSYKRAAKLQQQGSVSIAANDIKSDMPQSASQVGTPQAAITPTIVHSIQQPQGINK
VAE

Sequences:

>Translated_403_residues
MLQTEQTLFEQMRITELEIDFRKSLFSFTLADVRALQSFKPVIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAEINNSVADDLQNERIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAERYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQEIGTRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLCAVAQAIKESTRVEDHSFRYGGDEFCIIMPNCREDQARDNFIERFNQNIKLHLNNISLSY
GIVDTGPYDYDDANTLIHKADQKMYSYKRAAKLQQQGSVSIAANDIKSDMPQSASQVGTPQAAITPTIVHSIQQPQGINK
VAE
>Mature_403_residues
MLQTEQTLFEQMRITELEIDFRKSLFSFTLADVRALQSFKPVIEENIDKIVDDFYGLQTSVSEIALLIGDSDTLARLRTA
QRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLSAVHTLKELIYAEINNSVADDLQNERIRVAIDKLVLFDVTL
VFDTYIRSLVSEIENAKDKAERYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQEIGTRILRAAQRRAEPVSVIYLD
VDDFKSFNDTQGHRSGDEVLCAVAQAIKESTRVEDHSFRYGGDEFCIIMPNCREDQARDNFIERFNQNIKLHLNNISLSY
GIVDTGPYDYDDANTLIHKADQKMYSYKRAAKLQQQGSVSIAANDIKSDMPQSASQVGTPQAAITPTIVHSIQQPQGINK
VAE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=38.7878787878788, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=251, Percent_Identity=29.4820717131474, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081881, Length=109, Percent_Identity=40.3669724770642, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=27.810650887574, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=31.9767441860465, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=87, Percent_Identity=43.6781609195402, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=30.8139534883721, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787262, Length=112, Percent_Identity=30.3571428571429, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=33.75, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788381, Length=111, Percent_Identity=31.5315315315315, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45902; Mature: 45902

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTEQTLFEQMRITELEIDFRKSLFSFTLADVRALQSFKPVIEENIDKIVDDFYGLQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIALLIGDSDTLARLRTAQRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLS
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHH
AVHTLKELIYAEINNSVADDLQNERIRVAIDKLVLFDVTLVFDTYIRSLVSEIENAKDKA
HHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQEIGTRILRAAQRRAEPVSVIYLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDDFKSFNDTQGHRSGDEVLCAVAQAIKESTRVEDHSFRYGGDEFCIIMPNCREDQARDN
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHH
FIERFNQNIKLHLNNISLSYGIVDTGPYDYDDANTLIHKADQKMYSYKRAAKLQQQGSVS
HHHHHCCCEEEEEEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IAANDIKSDMPQSASQVGTPQAAITPTIVHSIQQPQGINKVAE
EEHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MLQTEQTLFEQMRITELEIDFRKSLFSFTLADVRALQSFKPVIEENIDKIVDDFYGLQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSEIALLIGDSDTLARLRTAQRRYVLDLFNGVYDLEYVNNRLRIGLVHKRIGVEPKLYLS
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHH
AVHTLKELIYAEINNSVADDLQNERIRVAIDKLVLFDVTLVFDTYIRSLVSEIENAKDKA
HHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERYAQSMESKVKERTQQLEELSQTDPLTGLLNVRHLQEIGTRILRAAQRRAEPVSVIYLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
VDDFKSFNDTQGHRSGDEVLCAVAQAIKESTRVEDHSFRYGGDEFCIIMPNCREDQARDN
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHH
FIERFNQNIKLHLNNISLSYGIVDTGPYDYDDANTLIHKADQKMYSYKRAAKLQQQGSVS
HHHHHCCCEEEEEEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IAANDIKSDMPQSASQVGTPQAAITPTIVHSIQQPQGINKVAE
EEHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]