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The map label for this gene is fadA [H]

Identifier: 117918482

GI number: 117918482

Start: 28251

End: 29414

Strand: Reverse

Name: fadA [H]

Synonym: Shewana3_0023

Alternate gene names: 117918482

Gene position: 29414-28251 (Counterclockwise)

Preceding gene: 117918483

Following gene: 117918481

Centisome position: 0.59

GC content: 50.86

Gene sequence:

>1164_bases
ATGAAACAAGCTGTTATCGTAGATTGCATTCGTACTCCTATGGGCCGTTCTAAGGCTGGGGTGTTTCGTAATGTACGTGC
AGAAACACTCTCTGCCGAATTAATGAAAGGTCTATTACTGCGTAACCCGCAATTAGATCCCAATACTATTGAAGATGTGA
TTTGGGGCTGCGTGCAGCAAACCCTAGAGCAAGGCTTCAACATTGCCCGTAACGCATCATTGCTGGCAGGTATCCCGAAG
ACGGCTGGCGCTGTGACAGTCAACCGCTTATGTGGTTCTTCGATGGAAGCCATTCATCAAGCCGCTCGTGCAATCATGAC
TGGCATGGGCGACACCTTTATCATCGGTGGTGTTGAACACATGGGCCACGTGCCAATGAACCACGGTGTGGACTTCCACC
CAGGTTTAGCCAATAACGTCGCTAAAGCGTCTGGCATGATGGGTTTAACCGCTGAGATGTTGGGTAAGCTTCACGGTATT
ACCCGTGAGCAGCAAGATGCCTTTGCAGTGCGTTCACACCAACGCGCTCACGCAGCGACAGTTGAAGGCCGCTTTGCCAA
GGAAATCTATGGCATCGAAGGCCATGATGCTAACGGCGCGCTAATTAAAGTGCTGCACGACGAAGTGATTCGTCCAGAAA
CCTCAATGGAGTCATTGGCGGCGCTGCGCCCAGTATTCGACCCAGCAAACGGTACTGTTACTGCCGGTACCTCTTCAGCA
TTATCTGACGGTGCTTCGGCAATGTTAGTGATGGAAGAATCTAAAGCGCGCGCTTTGGGTCTACCAATCCGTGCCCGTAT
CCGCTCAATGGCAGTTGCAGGTTGTGACGCGGCAATCATGGGTTACGGTCCAGTACCTGCTACTCAAAAAGCACTGGCTC
GCGCAGGCATCACAGTTAATGATTTGGATGTTATCGAGCTAAACGAAGCGTTCGCTGCTCAGTCTCTACCCTGCGTTAAA
GACCTAGGTCTGCTCGATGTGGTAGAAGACAAGATCAACCTAAATGGTGGCGCGATTGCGCTTGGCCATCCACTGGGCTG
TTCTGGTGCACGTATTTCGACAACGCTTATCAACCTGATGGAACACAAAGATGCGACCTTAGGTTTAGCCACTATGTGTA
TCGGTTTAGGCCAAGGTATTGCGACCGTTTTCGAACGCGTTTAA

Upstream 100 bases:

>100_bases
CACTTAGGTGGTTTGTATCAAGTCACCGACGCCATGCGCGCCCTTGCAGCCAACAACGGTAGCTACTACCAAGCCTAATT
AGCGGGAAAGGAATTTAATT

Downstream 100 bases:

>100_bases
TCCTTAACGATTCTTCCTTCAAAGCCGGACTCAGTTCCGGCTTTTTTATTTGCGATAGCGCCTTAATTATCTTCCTTTCT
ACTCAGCTTCAATTTGAAAA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQTLEQGFNIARNASLLAGIPK
TAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEHMGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGI
TREQQDAFAVRSHQRAHAATVEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA
LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVNDLDVIELNEAFAAQSLPCVK
DLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLMEHKDATLGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQTLEQGFNIARNASLLAGIPK
TAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEHMGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGI
TREQQDAFAVRSHQRAHAATVEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA
LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVNDLDVIELNEAFAAQSLPCVK
DLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLMEHKDATLGLATMCIGLGQGIATVFERV
>Mature_387_residues
MKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQTLEQGFNIARNASLLAGIPK
TAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEHMGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGI
TREQQDAFAVRSHQRAHAATVEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA
LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVNDLDVIELNEAFAAQSLPCVK
DLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLMEHKDATLGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=40.5128205128205, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=36.9346733668342, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.0352644836272, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI4504327, Length=430, Percent_Identity=31.6279069767442, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=32.4120603015075, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194353979, Length=238, Percent_Identity=32.7731092436975, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=71.5762273901809, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787663, Length=406, Percent_Identity=45.5665024630542, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=43.8902743142145, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.14463840399, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=33.8199513381995, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=361, Percent_Identity=38.5041551246537, Blast_Score=237, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=34.9127182044888, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=31.1778290993072, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=32.0802005012531, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=27.5510204081633, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=37.7215189873418, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=34.8148148148148, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=37.6558603491272, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17648125, Length=363, Percent_Identity=37.1900826446281, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=33.0210772833724, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=32.0802005012531, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40654; Mature: 40654

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQ
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNASLLAGIPKTAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEH
HHHHCCCHHCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA
HCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVN
HHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCEEC
DLDVIELNEAFAAQSLPCVKDLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLM
CCEEEECCHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EHKDATLGLATMCIGLGQGIATVFERV
HHCCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQ
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNASLLAGIPKTAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEH
HHHHCCCHHCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA
HCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVN
HHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCEEC
DLDVIELNEAFAAQSLPCVKDLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLM
CCEEEECCHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EHKDATLGLATMCIGLGQGIATVFERV
HHCCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA