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The map label for this gene is gidB [H]

Identifier: 117918460

GI number: 117918460

Start: 39

End: 659

Strand: Reverse

Name: gidB [H]

Synonym: Shewana3_0001

Alternate gene names: 117918460

Gene position: 659-39 (Counterclockwise)

Preceding gene: 117918461

Following gene: NA

Centisome position: 0.01

GC content: 47.18

Gene sequence:

>621_bases
GTGTTATCTGCCCAGTTAGAGGCCTATTTAGCCGAAATTAATCTGCCCGCCACGGCTGAGCAGAAAAAACAACTGCTTGA
TTTTGTTGGCATGCTCAACAAATGGAATAAAGCCTATAACCTAACTTCGGTAAGAGATCCTGAGGCGATGCTTGTCCGTC
ATATCATGGACAGCTTAGTGGTGTCGCCCCATTTGCAGGGTGAGCGCTTTATCGATGTAGGTACTGGCCCTGGGCTGCCC
GGCATTCCCTTAGCGATTATGAATCCAGATAAAACCTTTGTGCTGCTCGATAGCTTAGGCAAACGTATCCGTTTTCAAAA
GCAAGTGGCCTTTGAACTCGGGATCCACAATATTAGTTCGATAGAAAGCCGAGTCGAGGCCTATCAACCCGAGCAAAAGT
TCGATGGAGTGCTCAGCCGCGCCTTTGCCTCAATCCATGATATGTTGACATGGTGCCATCACTTGCCTGCCGAGCATGGA
CAATTTTATGCGCTCAAGGGACTACTGAGTGATGAAGAGATGCAACAAATCCCCGCTGGATTTGTGGTGACCGAAACCAT
CGAACTCCAAGTGCCTAGACTCGATGAGCAAAGACATTTACTCAAAATAATCAAAGAATAA

Upstream 100 bases:

>100_bases
AGTTTGGTCGCAGAAGGGAAGCATTCAGGCTTCCCTTCGACGTTTCAGGGCTTCATAATAGCCAGCGAGTTTATAATCGT
ATCTTTATTGAGGTTGTTCA

Downstream 100 bases:

>100_bases
CAGGCTTAAAGCTCCTCATTTCATATGGATTTTTAAGTAGGCAATAAACCAGAATTTACATACAGGGTGACATTGTGGGT
AAAGTAATTGCCGTGGCCAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLSAQLEAYLAEINLPATAEQKKQLLDFVGMLNKWNKAYNLTSVRDPEAMLVRHIMDSLVVSPHLQGERFIDVGTGPGLP
GIPLAIMNPDKTFVLLDSLGKRIRFQKQVAFELGIHNISSIESRVEAYQPEQKFDGVLSRAFASIHDMLTWCHHLPAEHG
QFYALKGLLSDEEMQQIPAGFVVTETIELQVPRLDEQRHLLKIIKE

Sequences:

>Translated_206_residues
MLSAQLEAYLAEINLPATAEQKKQLLDFVGMLNKWNKAYNLTSVRDPEAMLVRHIMDSLVVSPHLQGERFIDVGTGPGLP
GIPLAIMNPDKTFVLLDSLGKRIRFQKQVAFELGIHNISSIESRVEAYQPEQKFDGVLSRAFASIHDMLTWCHHLPAEHG
QFYALKGLLSDEEMQQIPAGFVVTETIELQVPRLDEQRHLLKIIKE
>Mature_206_residues
MLSAQLEAYLAEINLPATAEQKKQLLDFVGMLNKWNKAYNLTSVRDPEAMLVRHIMDSLVVSPHLQGERFIDVGTGPGLP
GIPLAIMNPDKTFVLLDSLGKRIRFQKQVAFELGIHNISSIESRVEAYQPEQKFDGVLSRAFASIHDMLTWCHHLPAEHG
QFYALKGLLSDEEMQQIPAGFVVTETIELQVPRLDEQRHLLKIIKE

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=199, Percent_Identity=59.2964824120603, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23282; Mature: 23282

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQLEAYLAEINLPATAEQKKQLLDFVGMLNKWNKAYNLTSVRDPEAMLVRHIMDSLV
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLQGERFIDVGTGPGLPGIPLAIMNPDKTFVLLDSLGKRIRFQKQVAFELGIHNISS
CCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHH
IESRVEAYQPEQKFDGVLSRAFASIHDMLTWCHHLPAEHGQFYALKGLLSDEEMQQIPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCC
FVVTETIELQVPRLDEQRHLLKIIKE
EEEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLSAQLEAYLAEINLPATAEQKKQLLDFVGMLNKWNKAYNLTSVRDPEAMLVRHIMDSLV
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLQGERFIDVGTGPGLPGIPLAIMNPDKTFVLLDSLGKRIRFQKQVAFELGIHNISS
CCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHH
IESRVEAYQPEQKFDGVLSRAFASIHDMLTWCHHLPAEHGQFYALKGLLSDEEMQQIPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCC
FVVTETIELQVPRLDEQRHLLKIIKE
EEEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA