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The map label for this gene is mnmG [H]

Identifier: 117918461

GI number: 117918461

Start: 777

End: 2666

Strand: Reverse

Name: mnmG [H]

Synonym: Shewana3_0002

Alternate gene names: 117918461

Gene position: 2666-777 (Counterclockwise)

Preceding gene: 117918462

Following gene: 117918460

Centisome position: 0.05

GC content: 47.72

Gene sequence:

>1890_bases
ATGCATTTTCATGAACGGTTTGATGTTATTGTTGTAGGTGGTGGTCATGCCGGAACTGAAGCCGCATTGGCTGCAGCAAG
AATGGGCTCTAAGACATTATTGCTGACGCATAATCTTGATACTTTAGGCCAAATGTCATGCAATCCTGCGATCGGCGGTA
TTGGCAAAGGTCATCTGGTAAAAGAAATCGATGCCTTAGGCGGTGCAATGGCGATCGCAACCGACTATGCAGGGATCCAA
TTTAGAACCCTAAACTCGAGCAAAGGTCCAGCAGTAAGAGCGACTCGTGCTCAGGCTGATCGCGCTTTGTATCGTCAGAA
GATCCAAAATATTCTGCAAAACCAAGCCAACTTACGTATTTTCCAACAAGCCGTTGATGATCTGGTGGTTGATAACGATC
GCGTAGTCGGCGTAGTGACCCAAATGGGCTTAGCCTTCGAAGCGCCTGCAGTGGTCTTAACCGCGGGGACTTTCCTCAGT
GGTAAAATTCACATTGGTTTAGAAAACTACAGTGGCGGTCGTGCTGGTGATCCACCATCTATCGCACTCGCCCACCGTTT
ACGTGAGTTGCCAATCCGTGTTGGCCGCTTAAAAACGGGTACGCCACCACGTATCGATGCCAATACCATCGATTTTACCC
AGATGACTGAGCAGAAAGGCGACACGCCATTACCTGTGATGTCGTTTATGGGCGATGTAAGCCATCACCCGAAACAAATT
TCTTGTTGGATCACCCATACCAATGAAAAGACACACGATATTATTCGTGGAGGCTTAGATCGCAGCCCGATGTATTCAGG
CGTGATCGAAGGGATTGGTCCACGTTACTGCCCTTCTATCGAAGACAAGATCCATCGTTTTTCAGATAAATCATCACACC
AGATCTTTATCGAACCCGAAGGCTTAAACACCAACGAGATCTACCCTAACGGTATTTCCACCAGCTTGCCTTTCGATGTG
CAGTTAAATCTGGTTCGTTCGATCCAAGGTATGGAAAATGCTGAGATTGTTCGCCCAGGTTATGCGATTGAGTATGATTA
TTTCGATCCAAGGGATCTTAAAAACTCGTTAGAAACCAAAACCATCAATGGTTTGTTCTTCGCGGGCCAAATCAACGGTA
CCACGGGTTATGAAGAAGCCGGCGCCCAAGGTTTACTCGCGGGGATGAACGCTGCACTGCAGGTTCAGGGCAAAGAAGCT
TGGTGTCCTCGCCGTGATGAAGCCTACATAGGTGTATTGGTTGACGATTTATCAACCTTAGGGACTAAAGAACCCTACCG
CATGTTTACCAGCCGCGCTGAATACCGCCTATTGCTGCGTGAAGACAACGCCGACCTGCGTTTAACCGCAAAAGGTCGTG
AACTGGGTCTGGTGGATGATGCGCGTTGGGCCTCTTTCAGTGAAAAAATGGAATCGATCGAGTTAGAACTGCAACGTCTA
CGTAGCCAATGGATCCATCCAAATTCACCATTGGTGCCAGTGTTAAATCCGCATTTAAACACGCCTATCTCCCGTGAAGC
GTCGTTTGAAGAGTTGCTGCGTCGTCCAGAAATGGATTACAGCAAGTTGATGCAGATTGAAGGTTTTGGTCCAGGACTTG
CCGATCCACTCGCAGCCGAGCAAGTACAGATCCAAGTGAAGTACTCTGGCTATATCCAACGTCAGCAGGAAGAGATCAAC
AAAGCGGTACGCAATGAGAACACTGGTTTGCCATTGAACCTTGATTACAAAGAGGTTCCAGGGCTTTCAAATGAGGTTAT
TGCTAAGTTAAATAGCCATAAGCCCGAAACTATTGGCCAAGCATCGCGTATCTCAGGGATTACGCCAGCAGCGATTTCAA
TTCTGCTGGTGCATCTGAAAAAACGCGGTTTATTACGCAAGAGCGCGTAA

Upstream 100 bases:

>100_bases
CTATGGATCCATTTTCTAAAAATGATCATTCACCTCATTCAAAGCAAATGCTAAAATGATCGGCTCACGTTGGGTCTCTT
AAGTTACTAAAGGTAGGGTC

Downstream 100 bases:

>100_bases
AAGCTAGCTTAATCGCCAGTTTGGTCGCAGAAGGGAAGCATTCAGGCTTCCCTTCGACGTTTCAGGGCTTCATAATAGCC
AGCGAGTTTATAATCGTATC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNLDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQANLRIFQQAVDDLVVDNDRVVGVVTQMGLAFEAPAVVLTAGTFLS
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFTQMTEQKGDTPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHDIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFSDKSSHQIFIEPEGLNTNEIYPNGISTSLPFDV
QLNLVRSIQGMENAEIVRPGYAIEYDYFDPRDLKNSLETKTINGLFFAGQINGTTGYEEAGAQGLLAGMNAALQVQGKEA
WCPRRDEAYIGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTAKGRELGLVDDARWASFSEKMESIELELQRL
RSQWIHPNSPLVPVLNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLADPLAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLNLDYKEVPGLSNEVIAKLNSHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA

Sequences:

>Translated_629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNLDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQANLRIFQQAVDDLVVDNDRVVGVVTQMGLAFEAPAVVLTAGTFLS
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFTQMTEQKGDTPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHDIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFSDKSSHQIFIEPEGLNTNEIYPNGISTSLPFDV
QLNLVRSIQGMENAEIVRPGYAIEYDYFDPRDLKNSLETKTINGLFFAGQINGTTGYEEAGAQGLLAGMNAALQVQGKEA
WCPRRDEAYIGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTAKGRELGLVDDARWASFSEKMESIELELQRL
RSQWIHPNSPLVPVLNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLADPLAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLNLDYKEVPGLSNEVIAKLNSHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA
>Mature_629_residues
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNLDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAIATDYAGIQ
FRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQANLRIFQQAVDDLVVDNDRVVGVVTQMGLAFEAPAVVLTAGTFLS
GKIHIGLENYSGGRAGDPPSIALAHRLRELPIRVGRLKTGTPPRIDANTIDFTQMTEQKGDTPLPVMSFMGDVSHHPKQI
SCWITHTNEKTHDIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFSDKSSHQIFIEPEGLNTNEIYPNGISTSLPFDV
QLNLVRSIQGMENAEIVRPGYAIEYDYFDPRDLKNSLETKTINGLFFAGQINGTTGYEEAGAQGLLAGMNAALQVQGKEA
WCPRRDEAYIGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTAKGRELGLVDDARWASFSEKMESIELELQRL
RSQWIHPNSPLVPVLNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLADPLAAEQVQIQVKYSGYIQRQQEEIN
KAVRNENTGLPLNLDYKEVPGLSNEVIAKLNSHKPETIGQASRISGITPAAISILLVHLKKRGLLRKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=642, Percent_Identity=46.1059190031153, Blast_Score=502, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=667, Percent_Identity=44.3778110944528, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=682, Percent_Identity=43.4017595307918, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=74.8807631160572, Blast_Score=986, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=638, Percent_Identity=40.7523510971787, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=621, Percent_Identity=47.1819645732689, Blast_Score=515, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24658174, Length=640, Percent_Identity=46.25, Blast_Score=513, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69266; Mature: 69266

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNLDTLGQMSCNPAIGGIGKGHLV
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAIATDYAGIQFRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQANLRI
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
FQQAVDDLVVDNDRVVGVVTQMGLAFEAPAVVLTAGTFLSGKIHIGLENYSGGRAGDPPS
HHHHHHHHEECCCCEEEEEHHCCCCCCCCEEEEEECCEEECEEEEEEECCCCCCCCCCCH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFTQMTEQKGDTPLPVMSFMGDVSHHPKQI
HHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
SCWITHTNEKTHDIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFSDKSSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GLNTNEIYPNGISTSLPFDVQLNLVRSIQGMENAEIVRPGYAIEYDYFDPRDLKNSLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHCCE
TINGLFFAGQINGTTGYEEAGAQGLLAGMNAALQVQGKEAWCPRRDEAYIGVLVDDLSTL
EECEEEEEEEECCCCCCHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTAKGRELGLVDDARWASFSEKMESIELELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHH
RSQWIHPNSPLVPVLNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLADPLAAE
HHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
QVQIQVKYSGYIQRQQEEINKAVRNENTGLPLNLDYKEVPGLSNEVIAKLNSHKPETIGQ
EEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEECCHHHCCCCCHHHHHHHCCCCCHHHCC
ASRISGITPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHFHERFDVIVVGGGHAGTEAALAAARMGSKTLLLTHNLDTLGQMSCNPAIGGIGKGHLV
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAIATDYAGIQFRTLNSSKGPAVRATRAQADRALYRQKIQNILQNQANLRI
HHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
FQQAVDDLVVDNDRVVGVVTQMGLAFEAPAVVLTAGTFLSGKIHIGLENYSGGRAGDPPS
HHHHHHHHEECCCCEEEEEHHCCCCCCCCEEEEEECCEEECEEEEEEECCCCCCCCCCCH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFTQMTEQKGDTPLPVMSFMGDVSHHPKQI
HHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
SCWITHTNEKTHDIIRGGLDRSPMYSGVIEGIGPRYCPSIEDKIHRFSDKSSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECC
GLNTNEIYPNGISTSLPFDVQLNLVRSIQGMENAEIVRPGYAIEYDYFDPRDLKNSLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHCCE
TINGLFFAGQINGTTGYEEAGAQGLLAGMNAALQVQGKEAWCPRRDEAYIGVLVDDLSTL
EECEEEEEEEECCCCCCHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTAKGRELGLVDDARWASFSEKMESIELELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHH
RSQWIHPNSPLVPVLNPHLNTPISREASFEELLRRPEMDYSKLMQIEGFGPGLADPLAAE
HHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
QVQIQVKYSGYIQRQQEEINKAVRNENTGLPLNLDYKEVPGLSNEVIAKLNSHKPETIGQ
EEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEECCHHHCCCCCHHHHHHHCCCCCHHHCC
ASRISGITPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA