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The map label for this gene is tnpR

Identifier: 117676258

GI number: 117676258

Start: 209233

End: 209793

Strand: Reverse

Name: tnpR

Synonym: Shewana3_4319

Alternate gene names: 117676258

Gene position: 209793-209233 (Counterclockwise)

Preceding gene: 117676259

Following gene: 117676257

Centisome position: 75.21

GC content: 57.75

Gene sequence:

>561_bases
ATGACTGGACAGCGCATTGGGTATATCAGGGTCAGCACCTTCGACCAGAACCCGGAACGGCAACTGGAAGGCGTCAAGGT
TGATCGCGCTTTTAGCGACAAGGCATCCGGCAAGGATGTCAAGCGTCCGCAACTGGAAGCGCTGATAAGCTTCGCCCGCA
CCGGCGACACCGTGGTGGTGCATAGCATGGATCGCCTGGCGCGCAATCTCGATGATTTGCGCCGGATCGTGCAAACGCTG
ACACAACGCGGCGTGCATATCGAATTCGTCAAGGAACACCTCAGTTTTACTGGCGAAGACTCTCCGATGGCGAACCTGAT
GCTCTCGGTGATGGGCGCGTTCGCCGAGTTCGAGCGCGCCCTGATCCGCGAGCGTCAGCGCGAGGGTATTGCGCTCGCCA
AGCAACGCGGGGCTTACCGTGGCAGGAAGAAATCCCTGTCGTCTGAGCGTATTGCCGAACTGCGCCAACGTGTCGAGGCT
GGCGAGCAAAAGACCAAGCTTGCTCGTGAATTCGGAATCAGTCGCGAAACCCTGTATCAATACTTGAGAACGGATCAGTA
A

Upstream 100 bases:

>100_bases
GCCATGTGTAAATTGCGTCAGGATAGGATTGAATTTTGAATTTATTGACATATCTCGTTGAAGGTCATAGAGTCTTCCCT
GACATTTTGCAGGGAATTCC

Downstream 100 bases:

>100_bases
ATATGCCACGTCGTTCCATCCTGTCCGCCGCCGAGCGGGAAAGCCTGCTGGCGTTGCCGGACTCCAAGGACGACCTGATC
CGACATTACACATTCAACGA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTL
TQRGVHIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
GEQKTKLAREFGISRETLYQYLRTDQ

Sequences:

>Translated_186_residues
MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTL
TQRGVHIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
GEQKTKLAREFGISRETLYQYLRTDQ
>Mature_185_residues
TGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLT
QRGVHIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAG
EQKTKLAREFGISRETLYQYLRTDQ

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=35.9550561797753, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787827, Length=187, Percent_Identity=33.1550802139037, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787638, Length=184, Percent_Identity=33.1521739130435, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TNR2_ECOLX (P04130)

Other databases:

- EMBL:   X01298
- EMBL:   M10791
- EMBL:   X04891
- PIR:   A03543
- RefSeq:   YP_001096360.1
- RefSeq:   YP_001096416.1
- ProteinModelPortal:   P04130
- SMR:   P04130
- GeneID:   4924685
- GeneID:   4924741
- ProtClustDB:   CLSK861775
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:3.40.50.1390
- SMART:   SM00857

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase; SSF46689 Homeodomain_like; SSF53041 Resolv_N

EC number: NA

Molecular weight: Translated: 21330; Mature: 21198

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS00397 RECOMBINASES_1; PS00398 RECOMBINASES_2

Important sites: ACT_SITE 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVV
CCCCEEEEEEEECCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQ
HHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
YLRTDQ
HHHCCC
>Mature Secondary Structure 
TGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFARTGDTVVV
CCCEEEEEEEECCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSPMANLMLSVMGAFAEFERA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQ
HHHHHHHCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
YLRTDQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2992807; 6312271; 3029727