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The map label for this gene is dacA [H]

Identifier: 116874163

GI number: 116874163

Start: 2782501

End: 2783322

Strand: Direct

Name: dacA [H]

Synonym: lwe2747

Alternate gene names: 116874163

Gene position: 2782501-2783322 (Clockwise)

Preceding gene: 116874138

Following gene: 116874166

Centisome position: 98.88

GC content: 35.77

Gene sequence:

>822_bases
ATGAAATTACGCAAACTAACTTGGTTGCTACTAATAGGAACATTGCTACTCTCCGCTTGCTCTGACAATCAAACAAATTT
AAACGTATCAGCAGACGCAGCAGCAGTCTATTCAATGAATGATAAAAAAGCTATATCTGAAAAAAATTCGGATAAAGTAA
TGCCAATAGCAAGTATCACAAAATTAATGACAACTTTCTTAGTACTAGAGGCTGTGAAAAAAGGCGAATTATCTTGGGAT
GAAAAACTTGATTTAATTCGACTAGATGATCCTTCAGCTGTTTCACTTTATGCAATTACAAAGAAAAGGACATGGTCTGT
AAGAGATTTATATAGTGCAATGCTTGTTATGTCTGCCAACGATGCTGCTGAAACACTTGGTAATCGGTTAGATGGAGCTG
ACTTCCCTAAAAAAATGAATAAAGAAGCGAAAAAACTCGGCTTATCTAACAAAACAGAATTTGTAAGCGCCAGTGGACTT
GATGTTGATGGAGAAAGGTCGGTTTCTACTACAAAGGACTTATACAAGTTATCTTCTGAATTAATTTCCACTTATCCTGA
AGTGCTAGACACAACTTCCAAATCAAGTATTATAACGGAAAAAGGTGCTAAAATTGAATCTACCAATGATTTAATAGGTA
CCATCCCTGGTTTAGACGGCTTAAAAACTGGATTTACAGATGAGGCCGGCTACTGCTTTATTGGTACAGCAAAACAAAAT
GGAAAACGTGTAATTTCCATAGTCTTAAATTCAGATACCGCCGAAAACCGATTTAAGGATACAGAGAAATTGATAAAACT
TGGGTTTAAAGGCGAGGAATAA

Upstream 100 bases:

>100_bases
AATTTTCAAGTTAAACCTTTAACTAATCCTTATATTTACTTGAATTTAACTCCATTTTTATCATGTATACTAAATTTAAT
ACTAAAAGGAGGAGAACAAA

Downstream 100 bases:

>100_bases
AATAAAAAGAACTTCTTTTGACGGAGAAGTTCTTTTTATATGGTCTAATCATTCACGTCGCAAAGCCCGAAGATATTTTT
CCAAATACATATCTTTATTT

Product: D-alanyl-D-alanine carboxypeptidase, putative

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKLRKLTWLLLIGTLLLSACSDNQTNLNVSADAAAVYSMNDKKAISEKNSDKVMPIASITKLMTTFLVLEAVKKGELSWD
EKLDLIRLDDPSAVSLYAITKKRTWSVRDLYSAMLVMSANDAAETLGNRLDGADFPKKMNKEAKKLGLSNKTEFVSASGL
DVDGERSVSTTKDLYKLSSELISTYPEVLDTTSKSSIITEKGAKIESTNDLIGTIPGLDGLKTGFTDEAGYCFIGTAKQN
GKRVISIVLNSDTAENRFKDTEKLIKLGFKGEE

Sequences:

>Translated_273_residues
MKLRKLTWLLLIGTLLLSACSDNQTNLNVSADAAAVYSMNDKKAISEKNSDKVMPIASITKLMTTFLVLEAVKKGELSWD
EKLDLIRLDDPSAVSLYAITKKRTWSVRDLYSAMLVMSANDAAETLGNRLDGADFPKKMNKEAKKLGLSNKTEFVSASGL
DVDGERSVSTTKDLYKLSSELISTYPEVLDTTSKSSIITEKGAKIESTNDLIGTIPGLDGLKTGFTDEAGYCFIGTAKQN
GKRVISIVLNSDTAENRFKDTEKLIKLGFKGEE
>Mature_273_residues
MKLRKLTWLLLIGTLLLSACSDNQTNLNVSADAAAVYSMNDKKAISEKNSDKVMPIASITKLMTTFLVLEAVKKGELSWD
EKLDLIRLDDPSAVSLYAITKKRTWSVRDLYSAMLVMSANDAAETLGNRLDGADFPKKMNKEAKKLGLSNKTEFVSASGL
DVDGERSVSTTKDLYKLSSELISTYPEVLDTTSKSSIITEKGAKIESTNDLIGTIPGLDGLKTGFTDEAGYCFIGTAKQN
GKRVISIVLNSDTAENRFKDTEKLIKLGFKGEE

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=263, Percent_Identity=31.9391634980989, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082022, Length=221, Percent_Identity=33.9366515837104, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=32.3886639676113, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786851, Length=288, Percent_Identity=26.3888888888889, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 29827; Mature: 29827

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRKLTWLLLIGTLLLSACSDNQTNLNVSADAAAVYSMNDKKAISEKNSDKVMPIASIT
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHCCCCCCCCCHHHHHH
KLMTTFLVLEAVKKGELSWDEKLDLIRLDDPSAVSLYAITKKRTWSVRDLYSAMLVMSAN
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC
DAAETLGNRLDGADFPKKMNKEAKKLGLSNKTEFVSASGLDVDGERSVSTTKDLYKLSSE
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
LISTYPEVLDTTSKSSIITEKGAKIESTNDLIGTIPGLDGLKTGFTDEAGYCFIGTAKQN
HHHHHHHHHCCCCCCCEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCC
GKRVISIVLNSDTAENRFKDTEKLIKLGFKGEE
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLRKLTWLLLIGTLLLSACSDNQTNLNVSADAAAVYSMNDKKAISEKNSDKVMPIASIT
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHCCCCCCCCCHHHHHH
KLMTTFLVLEAVKKGELSWDEKLDLIRLDDPSAVSLYAITKKRTWSVRDLYSAMLVMSAN
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC
DAAETLGNRLDGADFPKKMNKEAKKLGLSNKTEFVSASGLDVDGERSVSTTKDLYKLSSE
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
LISTYPEVLDTTSKSSIITEKGAKIESTNDLIGTIPGLDGLKTGFTDEAGYCFIGTAKQN
HHHHHHHHHCCCCCCCEECCCCCEECCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCC
GKRVISIVLNSDTAENRFKDTEKLIKLGFKGEE
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]